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Open AccessArticle

Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment

1
Department of Medicine, Royal Melbourne Hospital, The University of Melbourne, Victoria 3050, Australia
2
Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia
3
Department of Neurology, Royal Melbourne Hospital, Victoria 3050, Australia
4
Department of Electrical and Electronic Engineering, Melbourne School of Engineering, The University of Melbourne, Victoria 3010, Australia
5
Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Victoria 3010, Australia
*
Author to whom correspondence should be addressed.
These authors made equal contribution.
Sensors 2019, 19(16), 3543; https://doi.org/10.3390/s19163543
Received: 4 July 2019 / Revised: 30 July 2019 / Accepted: 11 August 2019 / Published: 14 August 2019
(This article belongs to the Section Biomedical Sensors)
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Abstract

Pre-treatment screening of individuals for human leukocyte antigens (HLA) HLA-B*57:01 is recommended for the prevention of life-threatening hypersensitivity reactions to abacavir, a drug widely prescribed for HIV treatment. However, the implementation of screening in clinical practice is hindered by the slow turnaround time and high cost of conventional HLA genotyping methods. We have developed a biosensor platform using interdigitated electrode (IDE) functionalized with a monoclonal antibody to detect cells expressing HLA-B*57:01. This platform was evaluated using cell lines and peripheral blood mononuclear cells expressing different HLA-B alleles. The functionalized IDE sensor was able to specifically capture HLA-B*57:01 cells, resulting in a significant change in the impedance magnitude in 20 min. This IDE platform has the potential to be further developed to enable point-of-care HLA-B*57:01 screening. View Full-Text
Keywords: human leukocyte antigen; interdigitated electrodes; impedance sensor; protein sensor; point-of-care diagnostics; pharmacogenetics human leukocyte antigen; interdigitated electrodes; impedance sensor; protein sensor; point-of-care diagnostics; pharmacogenetics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Chan, J.; Soraya, G.V.; Craig, L.; Uddin, S.M.; Todaro, M.; Huynh, D.H.; Abeyrathne, C.D.; Kostenko, L.; McCluskey, J.; Skafidas, E.; Kwan, P. Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment. Sensors 2019, 19, 3543.

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