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Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles

1
Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, China
2
College of Liberal Arts and Science, National University of Defense Technology, Changsha 410073, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Sensors 2019, 19(1), 40; https://doi.org/10.3390/s19010040
Received: 26 November 2018 / Revised: 17 December 2018 / Accepted: 19 December 2018 / Published: 22 December 2018
(This article belongs to the Special Issue Electrochemical Nanobiosensors )
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Abstract

A biosensor based on a combination of cytochrome c (Cyt c), electrochemical reduced graphene oxides (ERGO), and gold nanoparticles (AuNPs) on a glassy carbon electrode (GCE) was fabricated. The proposed biosensor electrode was denoted as GCE/ERGO-Nafion/AuNPs/Cyt c/Nafion, where ERGO-Nafion was deposited by dropping graphene oxides-Nafion mixed droplet first and following electrochemical reduction, AuNPs were directly deposited on the surface of the ERGO-Nafion modified electrode by electrochemical reduction, and other components were deposited by the dropping-dry method. The effect of the deposition amount of AuNPs on direct electrochemistry of Cyt c in the proposed electrode was investigated. The hydrogen peroxide was taken to evaluate the performance of the proposed biosensor. The results showed that the biosensor has great analytical performance, including a high sensitivity, a wide linear range, a low detection limit, and good stability, reproducibility, and reliability. View Full-Text
Keywords: biosensor; cytochrome c; graphene; gold nanoparticles; hydrogen peroxide; direct electrochemistry biosensor; cytochrome c; graphene; gold nanoparticles; hydrogen peroxide; direct electrochemistry
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Guo, C.; Wang, J.; Chen, X.; Li, Y.; Wu, L.; Zhang, J.; Tao, C.-A. Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles. Sensors 2019, 19, 40.

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