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Sensors 2018, 18(9), 2968; https://doi.org/10.3390/s18092968

Simple and Label-Free Fluorescent Detection of Melamine Based on Melamine–Thymine Recognition

1
Edible and Medicinal Fungi Research Center, Yangtze University, Jingzhou 434025, China
2
College of Life Science, Yangtze University, Jingzhou 434025, China
*
Author to whom correspondence should be addressed.
Received: 31 July 2018 / Revised: 28 August 2018 / Accepted: 2 September 2018 / Published: 6 September 2018
(This article belongs to the Special Issue Label-Free Biosensors)
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Abstract

In the past few years, melamine has been illegally added into dairy products to increase the apparent crude protein levels. If humans or animals drink the milk adulteration of melamine, it can form insoluble melamine–cyanurate crystals in their kidneys which causes kidney damage or even death. In the present work, we constructed a simple and label-free fluorescent method for melamine detection based on melamine-thymine recognition. SYBR Green I was utilized as a reporter for this method as it did not require any modification or expensive equipment. In the absence of melamine, polythymine DNA was digested by Exo I, which caused a decrease in the fluorescence signal. In the presence of melamine, the polythymine DNA was able to fold into a double chain structure, however this was done with the help of T-melamine-T mismatches to prevent degradation. Then, the SYBR Green I combined with the double-stranded DNA to result in an intense fluorescence signal. The limit of detection in this method was 1.58 μM, which satisfied the FDA standards. This method also had a good linear relationship within the range of 10–200 μM. In addition, this new method has a good selectivity to distinguish melamine from the component of milk. As a result, we developed a simple and highly selectivity method for melamine detection. View Full-Text
Keywords: melamine detection; melamine-thymine recognition; SYBR Green I; exonuclease I melamine detection; melamine-thymine recognition; SYBR Green I; exonuclease I
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Yang, H.; Wang, J.; Wu, Q.; Wang, Y.; Li, L.; Ding, B. Simple and Label-Free Fluorescent Detection of Melamine Based on Melamine–Thymine Recognition. Sensors 2018, 18, 2968.

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