Next Article in Journal
Second Iteration of Photogrammetric Processing to Refine Image Orientation with Improved Tie-Points
Next Article in Special Issue
A Simple and Effective Colorimetric Assay for Glucose Based on MnO2 Nanosheets
Previous Article in Journal
Acoustic Parametric Signal Generation for Underwater Communication
Previous Article in Special Issue
Coumarin Probe for Selective Detection of Fluoride Ions in Aqueous Solution and Its Bioimaging in Live Cells
Open AccessArticle

Simultaneous Detection of Adenosine Triphosphate and Glucose Based on the Cu-Fenton Reaction

by Fei Qu 1,2,*, Jingwen Li 1,2, Wenli Han 3, Lian Xia 1,2 and Jinmao You 1,2,4
1
The Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu 273165, China
2
Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu 273165, China
3
Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, China
4
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
*
Author to whom correspondence should be addressed.
Sensors 2018, 18(7), 2151; https://doi.org/10.3390/s18072151
Received: 28 May 2018 / Revised: 28 June 2018 / Accepted: 28 June 2018 / Published: 4 July 2018
(This article belongs to the Special Issue Colorimetric and Fluorescent Sensors 2018)
Both adenosine triphosphate (ATP) and glucose are important to human health, and their abnormal levels are closely related to angiocardiopathy and hypoglycaemia. Therefore, the simultaneous determination of ATP and glucose with a single test mode is highly desirable for disease diagnostics and early recognition. Herein, a new fluorescence on/off switch sensing platform is developed by carbon nanodots (CNDs) to detect ATP and glucose simultaneously. The fluorescence of CNDs can be quenched by Cu2+ and hydrogen peroxide (H2O2), due to the formation of hydroxyl radicals (·OH) produced in the Cu-Fenton reaction. Based on the high affinity of Cu2+ with ATP, the fluorescence of CNDs will recover effectively after adding ATP. Additionally, glucose can be efficiently catalyzed by glucose oxidase (GOx) to generate H2O2, so the platform can also be utilized to analyze glucose. Under optimum conditions, this sensing platform displays excellent sensitivity and the linear ranges are from 0.1 to 7 μM for ATP with a limit of detection (LOD) of 30.2 nM, and from 0.1 to 7 mM for glucose with a LOD 39.8 μM, respectively. Benefiting from the high sensitivity and selectivity, this sensing platform is successfully applied for simultaneous detection of ATP and glucose in human serum samples with satisfactory recoveries. View Full-Text
Keywords: simultaneous detection; Cu-Fenton reaction; carbon nanodots; adenosine triphosphate; glucose; satisfactory recoveries simultaneous detection; Cu-Fenton reaction; carbon nanodots; adenosine triphosphate; glucose; satisfactory recoveries
Show Figures

Figure 1

MDPI and ACS Style

Qu, F.; Li, J.; Han, W.; Xia, L.; You, J. Simultaneous Detection of Adenosine Triphosphate and Glucose Based on the Cu-Fenton Reaction. Sensors 2018, 18, 2151.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop