Next Article in Journal
A Strap-Down Inertial Navigation/Spectrum Red-Shift/Star Sensor (SINS/SRS/SS) Autonomous Integrated System for Spacecraft Navigation
Next Article in Special Issue
A Non-Label and Enzyme-Free Sensitive Detection Method for Thrombin Based on Simulation-Assisted DNA Assembly
Previous Article in Journal
An Exception Handling Approach for Privacy-Preserving Service Recommendation Failure in a Cloud Environment
Previous Article in Special Issue
A Microelectrode Array with Reproducible Performance Shows Loss of Consistency Following Functionalization with a Self-Assembled 6-Mercapto-1-hexanol Layer
Open AccessReview

On the Determination of Uncertainty and Limit of Detection in Label-Free Biosensors

1
Centre for Biomedical Technology, Optics, Photonics and Biophotonics Laboratory, Campus Montegancedo, Universidad Politécnica de Madrid, 28223 Madrid, Spain
2
Department of Applied Physics, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, C/José Gutiérrez Abascal, 2, 28006 Madrid, Spain
3
Laboratorio de Metrología y Metrotecnia, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, C/ José Gutiérrez Abascal, 2, 28006 Madrid, Spain
4
BioOptical Detection, Centro de Tecnología Biomédica, Campus Montegancedo, 28223 Madrid, Spain
*
Author to whom correspondence should be addressed.
Sensors 2018, 18(7), 2038; https://doi.org/10.3390/s18072038
Received: 27 April 2018 / Revised: 14 June 2018 / Accepted: 21 June 2018 / Published: 26 June 2018
(This article belongs to the Special Issue Label-Free Biosensors)
A significant amount of noteworthy articles reviewing different label-free biosensors are being published in the last years. Most of the times, the comparison among the different biosensors is limited by the procedure used of calculating the limit of detection and the measurement uncertainty. This article clarifies and establishes a simple procedure to determine the calibration function and the uncertainty of the concentration measured at any point of the measuring interval of a generic label-free biosensor. The value of the limit of detection arises naturally from this model as the limit at which uncertainty tends when the concentration tends to zero. The need to provide additional information, such as the measurement interval and its linearity, among others, on the analytical systems and biosensor in addition to the detection limit is pointed out. Finally, the model is applied to curves that are typically obtained in immunoassays and a discussion is made on the application validity of the model and its limitations. View Full-Text
Keywords: label-free biosensing performance; calculation of the limit of detection; limit of quantification; measurement uncertainty label-free biosensing performance; calculation of the limit of detection; limit of quantification; measurement uncertainty
Show Figures

Figure 1

MDPI and ACS Style

Lavín, Á.; Vicente, J.D.; Holgado, M.; Laguna, M.F.; Casquel, R.; Santamaría, B.; Maigler, M.V.; Hernández, A.L.; Ramírez, Y. On the Determination of Uncertainty and Limit of Detection in Label-Free Biosensors. Sensors 2018, 18, 2038.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop