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Sensors 2018, 18(5), 1616; https://doi.org/10.3390/s18051616

SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics

Institute of Biomedical Chemistry, 119121 Moscow, Russia
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Received: 18 April 2018 / Revised: 11 May 2018 / Accepted: 15 May 2018 / Published: 18 May 2018
(This article belongs to the Special Issue Label-Free Biosensors)
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Abstract

We have developed an original experimental approach based on the use of surface plasmon resonance (SPR) biosensors, applicable for investigation of potential partners involved in protein–protein interactions (PPI) as well as protein–peptide or protein–small molecule interactions. It is based on combining a SPR biosensor, size exclusion chromatography (SEC), mass spectrometric identification of proteins (LC-MS/MS) and direct molecular fishing employing principles of affinity chromatography for isolation of potential partner proteins from the total lysate of biological samples using immobilized target proteins (or small non-peptide compounds) as ligands. Applicability of this approach has been demonstrated within the frame of the Human Proteome Project (HPP) and PPI regulation by a small non-peptide biologically active compound, isatin. View Full-Text
Keywords: SPR biosensors; direct molecular fishing; protein interactomics; size exclusion chromatography (SEC); LC-MS/MS protein identification; isatin SPR biosensors; direct molecular fishing; protein interactomics; size exclusion chromatography (SEC); LC-MS/MS protein identification; isatin
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Florinskaya, A.; Ershov, P.; Mezentsev, Y.; Kaluzhskiy, L.; Yablokov, E.; Medvedev, A.; Ivanov, A. SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics. Sensors 2018, 18, 1616.

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