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Open AccessArticle

An Antibody-Immobilized Silica Inverse Opal Nanostructure for Label-Free Optical Biosensors

Hazards Monitoring Bionano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Korea
Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon 34113, Korea
BioNano Health-Guard Research Center, Global Frontier Project, 125 Gwahak-ro, Yuseong, Daejeon 34141, Korea
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea
Author to whom correspondence should be addressed.
Sensors 2018, 18(1), 307;
Received: 8 December 2017 / Revised: 16 January 2018 / Accepted: 16 January 2018 / Published: 20 January 2018
(This article belongs to the Special Issue Nanostructured Hybrid Materials Based Opto-Electronics Sensors)
PDF [2315 KB, uploaded 20 January 2018]


Three-dimensional SiO2-based inverse opal (SiO2-IO) nanostructures were prepared for use as biosensors. SiO2-IO was fabricated by vertical deposition and calcination processes. Antibodies were immobilized on the surface of SiO2-IO using 3-aminopropyl trimethoxysilane (APTMS), a succinimidyl-[(N-maleimidopropionamido)-tetraethyleneglycol] ester (NHS-PEG4-maleimide) cross-linker, and protein G. The highly accessible surface and porous structure of SiO2-IO were beneficial for capturing influenza viruses on the antibody-immobilized surfaces. Moreover, as the binding leads to the redshift of the reflectance peak, the influenza virus could be detected by simply monitoring the change in the reflectance spectrum without labeling. SiO2-IO showed high sensitivity in the range of 103–105 plaque forming unit (PFU) and high specificity to the influenza A (H1N1) virus. Due to its structural and optical properties, SiO2-IO is a promising material for the detection of the influenza virus. Our study provides a generalized sensing platform for biohazards as various sensing strategies can be employed through the surface functionalization of three-dimensional nanostructures. View Full-Text
Keywords: silica; inverse opal; optical biosensor; influenza virus silica; inverse opal; optical biosensor; influenza virus

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Lee, W.S.; Kang, T.; Kim, S.-H.; Jeong, J. An Antibody-Immobilized Silica Inverse Opal Nanostructure for Label-Free Optical Biosensors. Sensors 2018, 18, 307.

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