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Sensors 2018, 18(1), 191;

LED-CT Scan for pH Distribution on a Cross-Section of Cell Culture Medium

Department of Mechanical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
Department of Mechanical Engineering, National Chiao Tung University, 1001 University Road, Hsinchu 300, Taiwan
Author to whom correspondence should be addressed.
Received: 15 December 2017 / Revised: 8 January 2018 / Accepted: 9 January 2018 / Published: 11 January 2018
(This article belongs to the Section Physical Sensors)
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In cell culture, the pH of the culture medium is one of the most important conditions. However, the culture medium may have non-uniform pH distribution due to activities of cells and changes in the environment. Although it is possible to measure the pH distribution with an existing pH meter using distributed electrodes, the method involves direct contact with the medium and would greatly increase the risk of contamination. Here in this paper, we propose a computed tomography (CT) scan for measuring pH distribution using the color change of phenol red with a light-emitting diode (LED) light source. Using the principle of CT scan, we can measure pH distribution without contacting culture medium, and thus, decrease the risk of contamination. We have developed the device with a LED, an array of photo receivers and a rotation mechanism. The system is firstly calibrated with different shapes of wooden objects that do not pass light, we succeeded in obtaining their 3D topographies. The system was also used for measuring a culture medium with two different pH values, it was possible to obtain a pH distribution that clearly shows the boundary. View Full-Text
Keywords: pH measurement; cell culture; non-contact measurement pH measurement; cell culture; non-contact measurement

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Higashino, N.; Takayama, T.; Ito, H.; Horade, M.; Yamaguchi, Y.; Dylan Tsai, C.-H.; Kaneko, M. LED-CT Scan for pH Distribution on a Cross-Section of Cell Culture Medium. Sensors 2018, 18, 191.

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