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Sensors 2017, 17(9), 2018; https://doi.org/10.3390/s17092018

Characterizations of Anti-Alpha-Fetoprotein-Conjugated Magnetic Nanoparticles Associated with Alpha-Fetoprotein for Biomedical Applications

1
Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan
2
Department of Surgery and Hepatitis Research Center, National Taiwan University Hospital, Taipei 100, Taiwan
3
Graduate Institute of Clinical Medicine, National Taiwan University, Taipei 100, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 7 August 2017 / Revised: 31 August 2017 / Accepted: 1 September 2017 / Published: 3 September 2017
(This article belongs to the Special Issue Magnetic Materials Based Biosensors)
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Abstract

In this work, we report characterizations of biofunctionalized magnetic nanoparticles (BMNPs) associated with alpha-fetoprotein (AFP) for biomedical applications. The example BMNP in this study is anti-alpha-fetoprotein (anti-AFP) conjugated onto dextran-coated Fe3O4 labeled as Fe3O4-anti-AFP, and the target is AFP. We characterize magnetic properties, such as increments of magnetization ΔMH and effective relaxation time Δτeff in the reaction process. It is found that both ΔMH and Δτeff are enhanced when the concentration of AFP, ФAFP, increases. The enhancements are due to magnetic interactions among BMNPs in magnetic clusters, which contribute extra MH after the association with MH and in turn enhance τeff. The screening of patients carrying hepatocellular carcinoma (HCC) is verified via ΔMH/MH. The proposed method can be applied to detect a wide variety of analytes. The scaling characteristics of ΔMH/MH show the potential to develop a vibrating sample magnetometer system with low field strength for clinic applications. View Full-Text
Keywords: magnetic immunoassay; biofunctionalized magnetic nanoparticles; biomarker; alpha-fetoprotein; hepatocellular carcinoma; magnetization enhancement magnetic immunoassay; biofunctionalized magnetic nanoparticles; biomarker; alpha-fetoprotein; hepatocellular carcinoma; magnetization enhancement
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Liao, S.-H.; Huang, H.-S.; Chieh, J.-J.; Su, Y.-K.; Tong, Y.-F.; Huang, K.-W. Characterizations of Anti-Alpha-Fetoprotein-Conjugated Magnetic Nanoparticles Associated with Alpha-Fetoprotein for Biomedical Applications. Sensors 2017, 17, 2018.

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