Next Article in Journal
Design and Implementation of a Smart Home System Using Multisensor Data Fusion Technology
Next Article in Special Issue
Theoretical Studies on Two-Photon Fluorescent Hg2+ Probes Based on the Coumarin-Rhodamine System
Previous Article in Journal
A Pulse Rate Detection Method for Mouse Application Based on Multi-PPG Sensors
Previous Article in Special Issue
Thiolate-Capped CdSe/ZnS Core-Shell Quantum Dots for the Sensitive Detection of Glucose
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Sensors 2017, 17(7), 1630; https://doi.org/10.3390/s17071630

A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis

1
Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
2
Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-naka, Kita-ku, Okayama 700-8530, Japan
*
Author to whom correspondence should be addressed.
Received: 16 May 2017 / Revised: 5 July 2017 / Accepted: 13 July 2017 / Published: 15 July 2017
(This article belongs to the Special Issue Fluorescent Probes and Sensors)
Full-Text   |   PDF [2776 KB, uploaded 15 July 2017]   |  

Abstract

A new fluorescent arrayed biosensor has been developed to discriminate species and concentrations of target proteins by using plural different phospholipid liposome species encapsulating fluorescent molecules, utilizing differences in permeation of the fluorescent molecules through the membrane to modulate liposome-target protein interactions. This approach proposes a basically new label-free fluorescent sensor, compared with the common technique of developed fluorescent array sensors with labeling. We have confirmed a high output intensity of fluorescence emission related to characteristics of the fluorescent molecules dependent on their concentrations when they leak from inside the liposomes through the perturbed lipid membrane. After taking an array image of the fluorescence emission from the sensor using a CMOS imager, the output intensities of the fluorescence were analyzed by a principal component analysis (PCA) statistical method. It is found from PCA plots that different protein species with several concentrations were successfully discriminated by using the different lipid membranes with high cumulative contribution ratio. We also confirmed that the accuracy of the discrimination by the array sensor with a single shot is higher than that of a single sensor with multiple shots. View Full-Text
Keywords: biosensor; fluorescence; liposome; protein; array; interaction; cholesterol; principal component analysis (PCA) biosensor; fluorescence; liposome; protein; array; interaction; cholesterol; principal component analysis (PCA)
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Imamura, R.; Murata, N.; Shimanouchi, T.; Yamashita, K.; Fukuzawa, M.; Noda, M. A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis. Sensors 2017, 17, 1630.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top