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Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

1 and 2,*
Department of Clinical Laboratory, The Second Affiliated Hospital of Southeast University, Nanjing 210003, China
School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
Author to whom correspondence should be addressed.
Sensors 2017, 17(12), 2805;
Received: 5 October 2017 / Revised: 20 November 2017 / Accepted: 30 November 2017 / Published: 4 December 2017
(This article belongs to the Section Biosensors)
PDF [3599 KB, uploaded 4 December 2017]


Biosensors for proteins have shown attractive advantages compared to traditional techniques in clinical laboratory diagnosis. In virtue of modern fabrication modes and detection techniques, various immunosensing platforms have been reported on basis of the specific recognition between antigen-antibody pairs. In addition to profit from the development of nanotechnology and molecular biology, diverse fabrication and signal amplification strategies have been designed for detection of protein antigens, which has led to great achievements in fast quantitative and simultaneous testing with extremely high sensitivity and specificity. Besides antigens, determination of antibodies also possesses great significance for clinical laboratory diagnosis. In this review, we will categorize recent immunosensors for proteins by different detection techniques. The basic conception of detection techniques, sensing mechanisms, and the relevant signal amplification strategies are introduced. Since antibodies and antigens have an equal position to each other in immunosensing, all biosensing strategies for antigens can be extended to antibodies under appropriate optimizations. Biosensors for antibodies are summarized, focusing on potential applications in clinical laboratory diagnosis, such as a series of biomarkers for infectious diseases and autoimmune diseases, and an evaluation of vaccine immunity. The excellent performances of these biosensors provide a prospective space for future antibody-detection-based disease serodiagnosis. View Full-Text
Keywords: immunoassay; biosensors; protein biomarker; serodiagnosis. immunoassay; biosensors; protein biomarker; serodiagnosis.

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Liu, X.; Jiang, H. Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis. Sensors 2017, 17, 2805.

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