A Low-Noise Transimpedance Amplifier for BLM-Based Ion Channel Recording
AbstractHigh-throughput screening (HTS) using ion channel recording is a powerful drug discovery technique in pharmacology. Ion channel recording with planar bilayer lipid membranes (BLM) is scalable and has very high sensitivity. A HTS system based on BLM ion channel recording faces three main challenges: (i) design of scalable microfluidic devices; (ii) design of compact ultra-low-noise transimpedance amplifiers able to detect currents in the pA range with bandwidth >10 kHz; (iii) design of compact, robust and scalable systems that integrate these two elements. This paper presents a low-noise transimpedance amplifier with integrated A/D conversion realized in CMOS 0.35 μm technology. The CMOS amplifier acquires currents in the range ±200 pA and ±20 nA, with 100 kHz bandwidth while dissipating 41 mW. An integrated digital offset compensation loop balances any voltage offsets from Ag/AgCl electrodes. The measured open-input input-referred noise current is as low as 4 fA/√Hz at ±200 pA range. The current amplifier is embedded in an integrated platform, together with a microfluidic device, for current recording from ion channels. Gramicidin-A, α-haemolysin and KcsA potassium channels have been used to prove both the platform and the current-to-digital converter. View Full-Text
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Crescentini, M.; Bennati, M.; Saha, S.C.; Ivica, J.; de Planque, M.; Morgan, H.; Tartagni, M. A Low-Noise Transimpedance Amplifier for BLM-Based Ion Channel Recording. Sensors 2016, 16, 709.
Crescentini M, Bennati M, Saha SC, Ivica J, de Planque M, Morgan H, Tartagni M. A Low-Noise Transimpedance Amplifier for BLM-Based Ion Channel Recording. Sensors. 2016; 16(5):709.Chicago/Turabian Style
Crescentini, Marco; Bennati, Marco; Saha, Shimul C.; Ivica, Josip; de Planque, Maurits; Morgan, Hywel; Tartagni, Marco. 2016. "A Low-Noise Transimpedance Amplifier for BLM-Based Ion Channel Recording." Sensors 16, no. 5: 709.
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