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Sensors 2016, 16(3), 376;

Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains

Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Korea
Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea
Korea Mycobacterium Resource Center (KMRC), The Korean Institute of Tuberculosis, Cheongju-si 28158, Korea
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 28 January 2016 / Revised: 8 March 2016 / Accepted: 11 March 2016 / Published: 15 March 2016
(This article belongs to the Section Biosensors)
Full-Text   |   PDF [1288 KB, uploaded 15 March 2016]   |  


In 2013 alone, the death rate among the 9.0 million people infected with Mycobacterium tuberculosis (TB) worldwide was around 14%, which is unacceptably high. An empiric treatment of patients infected with TB or drug-resistant Mycobacterium tuberculosis (MDR-TB) strain can also result in the spread of MDR-TB. The diagnostic tools which are rapid, reliable, and have simple experimental protocols can significantly help in decreasing the prevalence rate of MDR-TB strain. We report the evaluation of the 9G technology based 9G DNAChips that allow accurate detection and discrimination of TB and MDR-TB-RIF. One hundred and thirteen known cultured samples were used to evaluate the ability of 9G DNAChip in the detection and discrimination of TB and MDR-TB-RIF strains. Hybridization of immobilized probes with the PCR products of TB and MDR-TB-RIF strains allow their detection and discrimination. The accuracy of 9G DNAChip was determined by comparing its results with sequencing analysis and drug susceptibility testing. Sequencing analysis showed 100% agreement with the results of 9G DNAChip. The 9G DNAChip showed very high sensitivity (95.4%) and specificity (100%). View Full-Text
Keywords: tuberculosis; drug-resistant tuberculosis; diagnosis; accuracy; sensitivity; specificity tuberculosis; drug-resistant tuberculosis; diagnosis; accuracy; sensitivity; specificity

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Song, K.-S.; Nimse, S.B.; Kim, H.J.; Yang, J.; Kim, T. Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains. Sensors 2016, 16, 376.

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