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Open AccessCommunication

Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor

1
Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan
2
Japan Society for the Promotion of Science (JSPS), Ichibancho, Chiyoda-ku, Tokyo 102-8471, Japan
3
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
4
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan
5
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
6
Advanced Science Research Laboratory, Saitama Institute of Technology, Fukaya, Saitama 369-0293, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Junichi Anzai
Sensors 2016, 16(12), 2033; https://doi.org/10.3390/s16122033
Received: 30 September 2016 / Revised: 26 November 2016 / Accepted: 28 November 2016 / Published: 30 November 2016
(This article belongs to the Special Issue Sensors for Glycoproteins and Glycated Proteins)
Herein, we report on the fabrication of an extended-gated organic field-effect transistor (OFET)-based immunosensor and its application in the detection of human chromogranin A (hCgA). The fabricated OFET device possesses an extended-gate electrode immobilized with an anti-CgA antibody. The titration results of hCgA showed that the electrical changes in the OFET characteristics corresponded to the glycoprotein recognition ability of the monoclonal antibody (anti-CgA). The observed sensitivity (detection limit: 0.11 µg/mL) and selectivity indicate that the OFET-based immunosensor can be potentially applied to the rapid detection of the glycoprotein concentration without any labeling. View Full-Text
Keywords: chromogranin A; glycoprotein; organic field-effect transistor; immunosensor; extended-gate; label-free; antibody immobilization; self-assembled monolayer chromogranin A; glycoprotein; organic field-effect transistor; immunosensor; extended-gate; label-free; antibody immobilization; self-assembled monolayer
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MDPI and ACS Style

Minamiki, T.; Minami, T.; Sasaki, Y.; Wakida, S.-I.; Kurita, R.; Niwa, O.; Tokito, S. Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor. Sensors 2016, 16, 2033.

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