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Article

A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate

1
School of Chemistry & CELEQ, University of Costa Rica, San Pedro de Montes de Oca, 11500-2060 San José, Costa Rica
2
Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain
*
Author to whom correspondence should be addressed.
Sensors 2015, 15(2), 2232-2243; https://doi.org/10.3390/s150202232
Received: 10 November 2014 / Revised: 31 December 2014 / Accepted: 13 January 2015 / Published: 22 January 2015
(This article belongs to the Special Issue Amperometric Biosensors)
This paper presents a chronoamperometric method to determine tungsten in water using screen-printed carbon electrodes modified with gold nanoparticles and cross linked alkaline phosphatase immobilized in the working electrode. Enzymatic activity over 2-phospho-l-ascorbic acid trisodium salt, used as substrate, was affected by tungsten ions, which resulted in a decrease of chronoamperometric current, when a potential of 200 mV was applied on 10 mM of substrate in a Tris HCl buffer pH 8.00 and 0.36 M of KCl. Calibration curves for the electrochemical method validation, give a reproducibility of 5.2% (n = 3), a repeatability of 9.4% (n = 3) and a detection limit of 0.29 ± 0.01 µM. Enriched tap water, purified laboratory water and bottled drinking water, with a certified tungsten reference solution traceable to NIST, gave a recovery of 97.1%, 99.1% and 99.1% respectively (n = 4 in each case) and a dynamic range from 0.6 to 30 µM. This study was performed by means of a Lineweaver–Burk plot, showing a mixed kinetic inhibition. View Full-Text
Keywords: alkaline phosphatase; chronoamperometry; 2-phospho-l-ascorbic acid trisodium salt; screen printed carbon electrode; tungsten alkaline phosphatase; chronoamperometry; 2-phospho-l-ascorbic acid trisodium salt; screen printed carbon electrode; tungsten
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MDPI and ACS Style

Alvarado-Gámez, A.L.; Alonso-Lomillo, M.A.; Domínguez-Renedo, O.; Arcos-Martínez, M.J. A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate. Sensors 2015, 15, 2232-2243. https://doi.org/10.3390/s150202232

AMA Style

Alvarado-Gámez AL, Alonso-Lomillo MA, Domínguez-Renedo O, Arcos-Martínez MJ. A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate. Sensors. 2015; 15(2):2232-2243. https://doi.org/10.3390/s150202232

Chicago/Turabian Style

Alvarado-Gámez, Ana L., María A. Alonso-Lomillo, Olga Domínguez-Renedo, and María J. Arcos-Martínez 2015. "A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substrate" Sensors 15, no. 2: 2232-2243. https://doi.org/10.3390/s150202232

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