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Open AccessArticle

Electrochemical Quantification of the Antioxidant Capacity of Medicinal Plants Using Biosensors

1
Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Área de Química Analítica, San Rafael Atlixco 186, Col. Vicentina, Del. Iztapalapa, México D.F., C.P. 09340, Mexico
2
Departamento de Materiales, Universidad Autónoma Metropolitana-Azcapotzalco, Área Ingeniería de Materiales, Av. San Pablo 180, Col. Reynosa-Tamaulipas, Del. Azcapotzalco, México, D.F., C.P. 02200, Mexico
3
SEP-Instituto Tecnológico de Toluca. Departamento de Ingeniería Química y Bioquímica. Av, Tecnológico S/N. Fraccionamiento La Virgen, Metepec, Edo de México, C.P. 52149, Mexico
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Sensors 2014, 14(8), 14423-14439; https://doi.org/10.3390/s140814423
Received: 30 June 2014 / Revised: 28 July 2014 / Accepted: 30 July 2014 / Published: 8 August 2014
(This article belongs to the Section Biosensors)
The working area of a screen-printed electrode, SPE, was modified with the enzyme tyrosinase (Tyr) using different immobilization methods, namely entrapment with water-soluble polyvinyl alcohol (PVA), cross-linking using glutaraldehyde (GA), and cross-linking using GA and human serum albumin (HSA); the resulting electrodes were termed SPE/Tyr/PVA, SPE/Tyr/GA and SPE/Tyr/HSA/GA, respectively. These biosensors were characterized by means of amperometry and EIS techniques. From amperometric evaluations, the apparent Michaelis-Menten constant, Km′, of each biosensor was evaluated while the respective charge transfer resistance, Rct, was assessed from impedance measurements. It was found that the SPE/Tyr/GA had the smallest Km′ (57 ± 7) µM and Rct values. This electrode also displayed both the lowest detection and quantification limits for catechol quantification. Using the SPE/Tyr/GA, the Trolox Equivalent Antioxidant Capacity (TEAC) was determined from infusions prepared with “mirto” (Salvia microphylla), “hHierba dulce” (Lippia dulcis) and “salve real” (Lippia alba), medicinal plants commonly used in Mexico. View Full-Text
Keywords: medicinal plants; antioxidant capacity; biosensors; tyrosinase; immobilization; screen-printed electrodes; Michaelis-Menten constant medicinal plants; antioxidant capacity; biosensors; tyrosinase; immobilization; screen-printed electrodes; Michaelis-Menten constant
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MDPI and ACS Style

Rodríguez-Sevilla, E.; Ramírez-Silva, M.-T.; Romero-Romo, M.; Ibarra-Escutia, P.; Palomar-Pardavé, M. Electrochemical Quantification of the Antioxidant Capacity of Medicinal Plants Using Biosensors. Sensors 2014, 14, 14423-14439. https://doi.org/10.3390/s140814423

AMA Style

Rodríguez-Sevilla E, Ramírez-Silva M-T, Romero-Romo M, Ibarra-Escutia P, Palomar-Pardavé M. Electrochemical Quantification of the Antioxidant Capacity of Medicinal Plants Using Biosensors. Sensors. 2014; 14(8):14423-14439. https://doi.org/10.3390/s140814423

Chicago/Turabian Style

Rodríguez-Sevilla, Erika; Ramírez-Silva, María-Teresa; Romero-Romo, Mario; Ibarra-Escutia, Pedro; Palomar-Pardavé, Manuel. 2014. "Electrochemical Quantification of the Antioxidant Capacity of Medicinal Plants Using Biosensors" Sensors 14, no. 8: 14423-14439. https://doi.org/10.3390/s140814423

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