Optimization of Hydrogen Peroxide Detection for a Methyl Mercaptan Biosensor
by
Zhan-Hong Li 1,2, Houssemeddine Guedri 2, Bruno Viguier 2, Shi-Gang Sun 1 and Jean-Louis Marty 2,*
Zhan-Hong Li 1,2, Houssemeddine Guedri 2, Bruno Viguier 2, Shi-Gang Sun 1 and Jean-Louis Marty 2,*
1
State Key Laboratory of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
2
Laboratoire IMAGES EA 4218, Groupe Biocapteurs, Université de Perpignan Via Domitia (UPVD), Bâtiment S, 52 av Paul Alduy 66860 Perpignan Cedex, France
*
Author to whom correspondence should be addressed.
Sensors 2013, 13(4), 5028-5039; https://doi.org/10.3390/s130405028
Received: 11 March 2013 / Revised: 3 April 2013 / Accepted: 7 April 2013 / Published: 15 April 2013
(This article belongs to the Special Issue Enzymatic Biosensors)
Several kinds of modified carbon screen printed electrodes (CSPEs) for amperometric detection of hydrogen peroxide (H2O2) are presented in order to propose a methyl mercaptan (MM) biosensor. Unmodified, carbon nanotubes (CNTs), cobalt phthalocyanine (CoPC), Prussian blue (PB), and Os-wired HRP modified CSPE sensors were fabricated and tested to detect H2O2, applying a potential of +0.6 V, +0.6 V, +0.4 V, −0.2 V and −0.1 V (versus Ag/AgCl), respectively. The limits of detection of these electrodes for H2O2 were 3.1 μM, 1.3 μM, 71 nM, 1.3 μM, 13.7 nM, respectively. The results demonstrated that the Os-wired HRP modified CSPEs gives the lowest limit of detection (LOD) for H2O2 at a working potential as low as −0.1 V. Os-wired HRP is the optimum choice for establishment of a MM biosensor and gives a detection limit of 0.5 μM.
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MDPI and ACS Style
Li, Z.-H.; Guedri, H.; Viguier, B.; Sun, S.-G.; Marty, J.-L. Optimization of Hydrogen Peroxide Detection for a Methyl Mercaptan Biosensor. Sensors 2013, 13, 5028-5039.
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