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Article

Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions

1
Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, 3590 Diepenbeek, Belgium
2
XIOS University College, Agoralaan building H, 3590 Diepenbeek, Belgium
3
Fraunhofer Institute for Non Destructive Testing (IZFP), Maria-Reiche-Strasse 2, 01109 Dresden, Germany
4
IMEC vzw, IMOMEC, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Sensors 2013, 13(11), 14650-14661; https://doi.org/10.3390/s131114650
Received: 28 August 2013 / Revised: 24 September 2013 / Accepted: 3 October 2013 / Published: 29 October 2013
(This article belongs to the Section Chemical Sensors)
In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integration, a proof of principle study was conducted to investigate any signs of interference between the two systems during a measurement. The flow cell was rinsed with 10 mM Tris-HCl and 1× PBS buffer in an alternating manner, while impedance and shifts of the resonance angle were monitored. After achieving a successful proof of principle, a usability test was conducted. It was assessed whether simultaneous detection occurred when: (i) Protein A is adsorbed to the gold surface of the chip; (ii) The non-occupied zone is blocked with BSA molecules and (iii) IgG1 is bound to the Protein A. The results indicate a successful merge between SPR and EIS. View Full-Text
Keywords: SPR; EIS; flow-cell; lab on chip; Protein A; immunoglobulin SPR; EIS; flow-cell; lab on chip; Protein A; immunoglobulin
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MDPI and ACS Style

Vandenryt, T.; Pohl, A.; Van Grinsven, B.; Thoelen, R.; De Ceuninck, W.; Wagner, P.; Opitz, J. Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions. Sensors 2013, 13, 14650-14661. https://doi.org/10.3390/s131114650

AMA Style

Vandenryt T, Pohl A, Van Grinsven B, Thoelen R, De Ceuninck W, Wagner P, Opitz J. Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions. Sensors. 2013; 13(11):14650-14661. https://doi.org/10.3390/s131114650

Chicago/Turabian Style

Vandenryt, Thijs, Andrea Pohl, Bart Van Grinsven, Ronald Thoelen, Ward De Ceuninck, Patrick Wagner, and Jörg Opitz. 2013. "Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions" Sensors 13, no. 11: 14650-14661. https://doi.org/10.3390/s131114650

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