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Assembly method fabricating linkers for covalently bonding DNA on glass surface

Key Lab of Molecular and Biomolecular electronics of Ministry of Education, Southeast University, Nanjing, 210096, P. R. China
Author to whom correspondence should be addressed.
Sensors 2001, 1(2), 53-59;
Received: 13 June 2001 / Accepted: 14 July 2001 / Published: 20 July 2001
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A fabrication method of linkers to covalently anchor nucleic acid probes (such as, oligonucleotides, PCR-products or peptide nucleic acid oligomers) on glass was developed by alternatively covalently assembling the different molecular components. The linkers with controllable length, flexibility and hydrophilicity could be prepared simply by dipping subtracts alternatively in different chemical solutions. A dialkoxy aminosilane (N-(2-aminoethyl)-3-aminopropylmethyl dimethoxylsilane, AEAPS) was chosen to substitute common used trialkoxy aminosilane to modify glass surface. The end groups of the linkers were formed as aldehyde or amino group, which were successfully used for attaching prefabricated DNA or in situ synthesis of oligonucleotides, respectively. The both experiments showed that the linker produce good reproducibility and uniformity of fluorescent hybridizing images, which can distinguish an internal single base mismatch in 20mer oligonucleotides. View Full-Text
Keywords: Linker; Oligonucleotide; Microarray; Silane Linker; Oligonucleotide; Microarray; Silane

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Li, J.; Wang, H.; Zhao, Y.; Cheng, L.; He, N.; Lu, Z. Assembly method fabricating linkers for covalently bonding DNA on glass surface. Sensors 2001, 1, 53-59.

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