Diversity 2014, 6(1), 72-87; https://doi.org/10.3390/d6010072
Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Marker Resources for Diversity Analysis of Mango (Mangifera indica L.)
Natalie L. Dillon 1,*
, David J. Innes 2, Ian S. E. Bally 1, Carole L. Wright 1, Luke C. Devitt 3,† and Ralf G. Dietzgen 4
1
Queensland Government Department of Agriculture, Fisheries and Forestry (DAFF), Agri-Science Queensland, Horticulture and Forestry Science, 28 Peters Street, Mareeba, QLD 4880, Australia
2
Queensland Government Department of Agriculture, Fisheries and Forestry (DAFF), Agri-Science Queensland, Crop and Food Science, Block 10, Level 1, Health and Food Sciences Precinct, 39 Kessels Road, Coopers Plains, QLD 4108, Australia
3
Queensland Government Department of Agriculture, Fisheries and Forestry (DAFF), Queensland Agricultural Biotechnology Centre, The University of Queensland, St. Lucia, QLD 4067, Australia
4
Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, St. Lucia, QLD 4067, Australia
†
Current address: Translational Research Institute, 37 Kent St, Woolloongabba, QLD 4102, Australia;
*
Author to whom correspondence should be addressed.
Received: 28 November 2013 / Revised: 7 January 2014 / Accepted: 7 January 2014 / Published: 20 January 2014
(This article belongs to the Special Issue Use of Molecular Markers in Genetic Diversity Research)
Abstract
In this study, a collection of 24,840 expressed sequence tags (ESTs) generated from five mango (Mangifera indica L.) cDNA libraries was mined for EST-based simple sequence repeat (SSR) markers. Over 1,000 ESTs with SSR motifs were detected from more than 24,000 EST sequences with di- and tri-nucleotide repeat motifs the most abundant. Of these, 25 EST-SSRs in genes involved in plant development, stress response, and fruit color and flavor development pathways were selected, developed into PCR markers and characterized in a population of 32 mango selections including M. indica varieties, and related Mangifera species. Twenty-four of the 25 EST-SSR markers exhibited polymorphisms, identifying a total of 86 alleles with an average of 5.38 alleles per locus, and distinguished between all Mangifera selections. Private alleles were identified for Mangifera species. These newly developed EST-SSR markers enhance the current 11 SSR mango genetic identity panel utilized by the Australian Mango Breeding Program. The current panel has been used to identify progeny and parents for selection and the application of this extended panel will further improve and help to design mango hybridization strategies for increased breeding efficiency. View Full-TextKeywords:
expressed sequence tag; simple sequence repeat; microsatellite; molecular marker; genetic diversity; cultivar identification
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Dillon, N.L.; Innes, D.J.; Bally, I.S.E.; Wright, C.L.; Devitt, L.C.; Dietzgen, R.G. Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Marker Resources for Diversity Analysis of Mango (Mangifera indica L.). Diversity 2014, 6, 72-87.
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