Diplostomum cf. vanelli Yamaguti, 1935 (Trematoda: Diplostomidae Poirier, 1886): Morpho-Molecular Data and Life Cycle
Abstract
1. Introduction
2. Materials and Methods
2.1. Life Cycle and Morphology of Worms
2.2. Molecular Data
2.2.1. DNA Extraction, Amplification, and Sequencing
2.2.2. Analysis of Genetic Data
Species | Sources | Genbank Accession Number |
---|---|---|
Diplostomum cf. vanelli | this study | PP002326 |
Diplostomum alascense | [21] | MZ314153 |
Diplostomum phoxini | [18] | AY222173 |
Diplostomum alarioides | [21] | MZ314152 |
Diplostomum scudderi | [21] | MZ314170 |
Diplostomum marshalli | [21] | MZ314167 |
Diplostomum gavium | [21] | MZ314154-55 MZ314157 |
Diplostomum pseudospathaceum | [22] | KR269766 |
Diplostomum rauschi | [21] | MZ314169 |
Diplostomum indistinctum | [21] | MZ314161-65 |
Diplostomum spathaceum | [22] | KR269765 |
[21] | MZ314171 | |
Diplostomum huronense | [21] | MZ314160 |
Diplostomum baeri | [23] | OK631869 |
Diplostomum ardeae | [24] | MT259036 |
Postharmostomum commutatum * | [25] | MH915390 |
Species | Sources | Genbank Accession Number |
---|---|---|
Diplostomum cf. vanelli | this study | PP002346 |
Diplostomum huronense | [21] [26] [8] [27] | MZ323257-61 GQ292488-90 KR271069; KR271071-74 HM064667-68; HM064672 |
Diplostomum rauschi | [21] | MZ323270 |
Diplostomum indistinctum | [21] [26] [27] [28] | MZ323262-63; MZ323265 GQ292482 HM064673 KT831379 |
Diplostomum baeri | Unpublished [29] [8] [30] [23] | MF142161; MF142171-73; MF142176; MF142178; MF142184; MF142186-89; MF142191; MF142196; MF142198; MF142209; MF142211-12; MF142214; MF142216; MF142222-24 MH368850-53 KR271040; KR271042-47; KR271050-60; KR271062; KR271064-67 KM212030-KM212031 OK632471-74; OK632476-77 |
Diplostomum spathaceum | [21] [8] [22] | MZ323274; MZ323276; MZ323278-81 KR271414-16; KR271419-24; KR271427-28; KR271431-45; KR271449; KR271451; KR271454-62; KR271465; KR271467-69 KR269763 |
Diplostomum alascense | [21] | MZ323250 |
Diplostomum scudderi | [21] | MZ323273 |
Diplostomum alarioides | [21] | MZ323249 |
Diplostomum pseudospathaceum | [22] [8] | KR269764 KR271083-89; KR271091-92 |
Diplostomum gavium | [21] | MZ323251; MZ323255 |
Diplostomum marshalli | [21] | MZ323268 |
Diplostomum lunaschiae | [24] | MT324602; MT324607-08; MT324612-14; MT324617; MT324621-22; MT324595-96; MT324599 |
Diplostomum ardeae | [24] [8] | MT324592-93 KR271033 |
Diplostomum mergi | [8] Unpublished | KR271082 KY271543 |
Postharmostomum commutatum * | [31] | MN200359 |
3. Results
3.1. Morphology Data
3.2. Molecular Data
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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DNA Region | Primer | Sequence 5′→3′ | Direction | Reference |
---|---|---|---|---|
28S | digl2 | AAGCATATCACTAAGCGG | forward, external | [15] |
1500R | GCTATCCTGAGGGAAACTTCG | reverse, external | [15] | |
900F | CCGTCTTGAAACACGGACCAAG | forward, internal | [15] | |
1200R | CTTGGTCCGTGTTTCAAGACGGG | reverse, internal | [15] | |
COX1 | MplatCOX1dF | TGTAAAACGACGGCCAGTTTWCITTRGATCATAAG | forward, external | [16] |
MplatCOX1dR | CAGGAAACAGCTATGACTGAAAYAAYAIIGGATCICCACC | reverse, external | [16] |
Diplostomum vanelli | |||
---|---|---|---|
Source | Present Study | Yamaguti 1935 cit. [32] | Dubois 1938 cit. [32] |
Body length | 785–1145 | 1210–1600 | 1200–1500 |
Prosoma length | 410–595 | 680–800 | 670–750 |
Prosoma width | 250–370 | 450–650 | 480–520 |
Opisthosoma length | 375–550 | 530–800 | 580–750 |
Opisthosoma width | 175–245 | 320–470 | 400–470 |
Oral sucker length | 50–80 | 48–75 | 55–62 |
Oral sucker width | 45–100 | 38–84 | 62–79 |
Lateral pseudosucker length | 55 | 90–102 diameter | 72–100 diameter |
Lateral pseudosucker width | 30–80 | ||
Pharynx length | 50–70 diameter | 54–66 | 53–65 |
Pharynx width | 42–54 | 45–50 | |
Ventral sucker length | 55–85 | 72–108 diameter | 74–82 |
Ventral sucker width | 70–105 | 86–90 | |
Holdfast organ length | 80–95 | 150–208 diameter | 220–280 diameter |
Holdfast organ width | 100–125 | ||
Anterior testis length | 100–220 | 110–180 | 140–160 |
Anterior testis width | 70–130 | 200–330 | 270–305 |
Posterior testis length | 175–220 | 140–200 | 150–205 |
Posterior testis width | 60–115 | 280–360 | 315–360 |
Ovary length | 50–60 | 50–110 | 105 |
Ovary width | 55–75 | 75–160 | 125–140 |
Eggs length | - | 93–108 | 96–104 |
Eggs width | - | 54–60 | 54–63 |
Prosoma/opisthosoma length ratio | 1.0 | - | 0.83–1.0 |
Oral/ventral sucker length ratio | 0.9 | - | - |
Species | Distances between Species | Distances within Species | ||||||
---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | ||||
1 | Diplostomum spathaceum | 0.0000 | 0.0008 | 0.0044 | 0.0039 | 0.0000 | 0.0000 | |
2 | Diplostomum cf. vanelli | 0.0000 | 0.0008 | 0.0044 | 0.0039 | - | - | |
3 | Diplostomum huronense | 0.0009 | 0.0009 | 0.0042 | 0.0038 | - | - | |
4 | Diplostomum baeri | 0.0188 | 0.0188 | 0.0179 | 0.0049 | - | - | |
5 | Diplostomum ardea | 0.0224 | 0.0224 | 0.0215 | 0.0323 | - | - |
Species | Distances between Species | Distances within Species | ||||
---|---|---|---|---|---|---|
1 | 2 | 3 | ||||
1 | Diplostomum cf. vanelli | 0.0126 | 0.0132 | - | - | |
2 | Diplostomum spathaceum | 0.0963 | 0.0126 | 0.0094 | 0.0021 | |
3 | Diplostomum indistinctum | 0.0849 | 0.0910 | 0.0082 | 0.0035 |
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Izrailskaia, A.V.; Besprozvannykh, V.V.; Shchelkanov, M.Y. Diplostomum cf. vanelli Yamaguti, 1935 (Trematoda: Diplostomidae Poirier, 1886): Morpho-Molecular Data and Life Cycle. Diversity 2024, 16, 286. https://doi.org/10.3390/d16050286
Izrailskaia AV, Besprozvannykh VV, Shchelkanov MY. Diplostomum cf. vanelli Yamaguti, 1935 (Trematoda: Diplostomidae Poirier, 1886): Morpho-Molecular Data and Life Cycle. Diversity. 2024; 16(5):286. https://doi.org/10.3390/d16050286
Chicago/Turabian StyleIzrailskaia, Anna V., Vladimir V. Besprozvannykh, and Michael Yu. Shchelkanov. 2024. "Diplostomum cf. vanelli Yamaguti, 1935 (Trematoda: Diplostomidae Poirier, 1886): Morpho-Molecular Data and Life Cycle" Diversity 16, no. 5: 286. https://doi.org/10.3390/d16050286
APA StyleIzrailskaia, A. V., Besprozvannykh, V. V., & Shchelkanov, M. Y. (2024). Diplostomum cf. vanelli Yamaguti, 1935 (Trematoda: Diplostomidae Poirier, 1886): Morpho-Molecular Data and Life Cycle. Diversity, 16(5), 286. https://doi.org/10.3390/d16050286