The Sea Slug Doriopsilla areolata Bergh, 1880 (Mollusca, Gastropoda) in the Mediterranean Sea: Another Case of Cryptic Diversity
Abstract
:1. Introduction
2. Materials and Methods
Molecular Analyses
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Voucher | Locality | Accession Numbers | ||
---|---|---|---|---|---|
H3 | 16S | COI | |||
Doriopsilla albopunctata | LACM:DISCO 11426 | White Point, California, USA (E-PAC) | - | - | MK550636 |
CPIC 00909 | Long Beach, California, USA (E-PAC) | - | KR002428 | KR002480 | |
CPIC 00915 | Long Beach, California, USA (E-PAC) | KR002524 | KR002429 | KR002481 | |
CPIC 00916 | Long Beach, California, USA (E-PAC) | - | KR002430 | KR002482 | |
CPIC 00917 | Long Beach, California, USA (E-PAC) | KR002525 | - | KR002483 | |
CPIC 00930 | Malibu, California, USA (E-PAC) | KR002527 | KR002431 | KR002485 | |
CPIC 00932 | Malibu, California, USA (E-PAC) | KR002528 | KR002432 | KR002486 | |
CPIC 01254 | Shell Beach, California, USA (E-PAC) | KR002535 | KR002440 | KR002494 | |
CPIC 01255 | Shell Beach, California, USA (E-PAC) | KR002536 | KR002441 | KR002495 | |
CPIC 01084 | Mission Bay, California, USA (E-PAC) | - | KR002442 | KR002496 | |
CPIC 01083 | Redondo Beach, California, USA (E-PAC) | - | KR002443 | KR002497 | |
CPIC 00918 | Long Beach, California, USA (E-PAC) | KR002526 | - | KR002484 | |
CPIC 01239 | Carpinteria, California, USA (E-PAC) | KR002534 | KR002439 | KR002493 | |
CPIC 00987 | Carpinteria, California, USA (E-PAC) | KR002529 | KR002434 | KR002488 | |
CPIC 00986 | Carpinteria, California, USA (E-PAC) | - | KR002433 | KR002487 | |
LACM:3420 | Carpinteria, California, USA (E-PAC) | KR002530 | KR002435 | KR002489 | |
- | Newport Beach, California, USA (E-PAC) | KR002531 | KR002436 | KR002490 | |
- | Newport Beach, California, USA (E-PAC) | KR002532 | KR002437 | KR002491 | |
- | Newport Beach, California, USA (E-PAC) | KR002533 | KR002438 | KR002492 | |
Doriopsilla areolata | RM3_109 | Hvar, Croatia (MED) | ON209460 | ON229526 | ON211997 |
RM3_1169 | Portopiccolo, Sistiana, Trieste, Italy (MED) | ON209466 | ON229532 | ON211996 | |
As D. areolata in GB | MNCN:15.05/23766 | Girona, Catalonia, Spain (MED) | KC171040 | KC171023 | - |
MNCN:15.05/23782 | Las Palmas, Gran Canaria, Spain (E-ATL) | KC171037 | KC171026 | - | |
LACM:2001-10.3 | Cadiz, Andalusia, Spain (E-ATL) | KC171035 | KC171024 | - | |
LACM:2001-10.4 | Cadiz, Andalusia, Spain (E-ATL) | KC171036 | KC171025 | - | |
MNCN:15.05/23784 | Angola (E-ATL) | KC171039 | KC171033 | - | |
MNCN:15.05/23789 | Angola (E-ATL) | KC171038 | KC171031 | - | |
Doriopsilla bertschi | CPIC 00976 | Bahia de los Angeles, Baja California, Mexico (E-PAC) | KR002551 | - | KR002515 |
CPIC 01058 | Bahia de los Angeles, Baja California, Mexico (E-PAC) | KR002552 | KR002462 | KR002517 | |
CPIC 01059 | Bahia de los Angeles, Baja California, Mexico (E-PAC) | - | KR002463 | KR002518 | |
LACM:140785 | Bahia de los Angeles, Baja California, Mexico (E-PAC) | KR002561 | KR002471 | KR002519 | |
LACM:3421 | Bahia de los Angeles, Baja California, Mexico (E-PAC) | KR002553 | KR002464 | - | |
Doriopsilla davebehrensi | CPIC 01038 | Bahia de los Angeles, Baja California, Mexico (E-PAC) | KR002564 | KR002475 | KR002520 |
LACM:3419 | Bahia de los Angeles, Baja California, Mexico (E-PAC) | KR002565 | KR002476 | KR002521 | |
LACM:76-5.6 | Bahia de los Angeles, Baja California, Mexico (E-PAC) | KR002566 | KR002478 | - | |
- | Newport Beach, California, USA (E-PAC) | - | KR002477 | KR002522 | |
Doriopsilla fulva | CPIC 00933 | Malibu, California, USA (E-PAC) | KR002537 | KR002444 | KR002498 |
CPIC 00934 | Malibu, California, USA (E-PAC) | KR002538 | KR002445 | KR002499 | |
CPIC 00936 | Malibu, California, USA (E-PAC) | KR002539 | KR002446 | KR002500 | |
CPIC 00937 | Malibu, California, USA (E-PAC) | - | KR002447 | KR002501 | |
CPIC 01240 | Mendocino, California, USA (E-PAC) | KR002541 | KR002449 | KR002503 | |
CPIC 01022 | Palos Verdes, California, USA (E-PAC) | KR002540 | KR002448 | KR002502 | |
Doriopsilla gemela | CPIC 00923 | Malibu, California, USA (E-PAC) | KR002542 | KR002450 | - |
CPIC 00924 | Malibu, California, USA (E-PAC) | KR002543 | KR002451 | KR002504 | |
CPIC 00931 | Malibu, California, USA (E-PAC) | - | KR002452 | KR002505 | |
CPIC 00938 | Malibu, California, USA (E-PAC) | KR002544 | KR002453 | KR002506 | |
CPIC 00939 | Malibu, California, USA (E-PAC) | KR002545 | KR002454 | KR002507 | |
CPIC 00978 | Carpinteria, California, USA (E-PAC) | KR002546 | KR002455 | KR002508 | |
CPIC 00979 | Carpinteria, California, USA (E-PAC) | KR002547 | KR002456 | KR002509 | |
CPIC 00980 | Carpinteria, California, USA (E-PAC) | - | KR002457 | KR002510 | |
CPIC 00981 | Carpinteria, California, USA (E-PAC) | - | KR002458 | KR002511 | |
CPIC 00982 | Carpinteria, California, USA (E-PAC) | KR002548 | KR002459 | KR002512 | |
CPIC 00983 | Carpinteria, California, USA (E-PAC) | KR002549 | KR002460 | KR002513 | |
CPIC 00984 | Carpinteria, California, USA (E-PAC) | KR002550 | KR002461 | KR002514 | |
Doriopsilla janaina | CPIC 00590 | Peru (E-PAC) | KC171034 | KC171022 | - |
Doriopsilla miniata | CAS:IZ:176370 | South Africa (E-ATL) | KC171043 | KC171030 | - |
CAS:IZ:176418 | Western Cape, South Africa (E-ATL) | KC171041 | KC171028 | - | |
CAS:IZ:176933 | Western Cape, South Africa (E-ATL) | KC171042 | KC171029 | - | |
D. pelseneeri | RM3_177 | Tarifa, Andalusia, Spain (E-ATL) | ON209459 | ON229525 | ON211995 |
As D. areolata in GB | - | Cadiz, Andalusia, Spain (E-ATL) | - | AJ225186 | AJ223262 |
- | Berlengas, Portugal (E-ATL) | - | KT820536 | KT833266 | |
- | Berlengas, Portugal (E-ATL) | - | - | KT833267 | |
D. rarispinosa | RM3_768 | Tavolara Island, Sardinia, Italy (MED) | ON209461 | ON229527 | ON211998 |
RM3_770 | Tavolara Island, Sardinia, Italy (MED) | ON209462 | ON229528 | ON211999 | |
RM3_771 | Tavolara Island, Sardinia, Italy (MED) | ON209463 | ON229529 | ON212000 | |
RM3_772 | Tavolara Island, Sardinia, Italy (MED) | ON209464 | ON229530 | ON212001 | |
RM3_498 | Kerkennah, Tunisia (MED) | ON209465 | ON229531 | ON212002 | |
RM3_1278 | Golfo di Olbia, Sardinia, Italy (MED) | ON209467 | ON229533 | ON212003 | |
RM3_1280 | Golfo di Olbia, Sardinia, Italy (MED) | ON209468 | ON229534 | ON212004 | |
RM3_1282 | Golfo di Olbia, Sardinia, Italy (MED) | ON209469 | ON229535 | ON212005 | |
RM3_1286 | Golfo di Olbia, Sardinia, Italy (MED) | ON209470 | ON229536 | ON212006 | |
RM3_2187 | Palavas-les-flots, Occitanie, France (MED) | ON209471 | ON229537 | ON212007 | |
RM3_2188 | Palavas-les-flots, Occitanie, France (MED) | ON209472 | ON229538 | ON212008 | |
RM3_2189 | Palavas-les-flots, Occitanie, France (MED) | ON209473 | ON229539 | - | |
RM3_2190 | L’Escala, Girona, Catalonia, Spain (MED) | ON209474 | ON229540 | ON212009 | |
RM3_2191 | L’Escala, Girona, Catalonia, Spain (MED) | ON209475 | ON229541 | ON212010 | |
Doriopsilla spaldingi | CPIC 00908 | San Pedro, California, USA (E-PAC) | KR002523 | KR002427 | KR002479 |
Doriopsilla sp. 1 | RM3_194 | Tarifa, Andalusia, Spain (E-ATL) | ON209458 | ON229524 | ON211994 |
Felimare tricolor | BAU 20547 | Secche di Tor Paterno MPA, Lazio, Italy (MED) | MK474153 | LN715193 | LN715211 |
Phyllidia coelestis | CAS:IZ:190982 | Kranket Island, Madang Prov., Papua New Guinea (W-PAC) | - | MF958279 | MF958412 |
Phco18LS1 | Lembeh, North Sulawesi, Strait Indonesia (IWP) | - | MK852557 | MK911039 | |
Phyllidia flava | RM3_546 | Giglio Island, Tuscany, Italy (MED) | ON209476 | - | ON212011 |
1. | 2. | 3. | 4. | 5. | 6. | 7. | 8. | 9. | 10. | 11. | 12. | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
1. D. albopunctata | - | |||||||||||
2. D. areolata | 0.18 | - | ||||||||||
3. D. bertschi | 0.16 | 0.16 | - | |||||||||
4. D. davebehrensi | 0.08 | 0.18 | 0.16 | - | ||||||||
5. D. fulva | 0.09 | 0.17 | 0.15 | 0.08 | - | |||||||
6. D. gemela | 0.15 | 0.16 | 0.10 | 0.15 | 0.15 | - | ||||||
7. D. pelseneeri | 0.17 | 0.03 | 0.16 | 0.18 | 0.16 | 0.17 | - | |||||
8. D. rarispinosa | 0.19 | 0.06 | 0.15 | 0.17 | 0.17 | 0.16 | 0.06 | - | ||||
9. D. spaldingi | 0.18 | 0.18 | 0.15 | 0.16 | 0.17 | 0.15 | 0.18 | 0.17 | - | |||
10. Doriopsilla sp. 1 | 0.21 | 0.09 | 0.17 | 0.20 | 0.20 | 0.16 | 0.09 | 0.09 | 0.18 | - | ||
11. Phyllidia spp. | 0.18 | 0.18 | 0.17 | 0.18 | 0.17 | 0.16 | 0.18 | 0.17 | 0.17 | 0.19 | - | |
12. Felimare tricolor | 0.22 | 0.18 | 0.19 | 0.22 | 0.21 | 0.19 | 0.17 | 0.17 | 0.19 | 0.19 | 0.19 |
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Furfaro, G.; Schreier, C.; Trainito, E.; Pontes, M.; Madrenas, E.; Girard, P.; Mariottini, P. The Sea Slug Doriopsilla areolata Bergh, 1880 (Mollusca, Gastropoda) in the Mediterranean Sea: Another Case of Cryptic Diversity. Diversity 2022, 14, 297. https://doi.org/10.3390/d14040297
Furfaro G, Schreier C, Trainito E, Pontes M, Madrenas E, Girard P, Mariottini P. The Sea Slug Doriopsilla areolata Bergh, 1880 (Mollusca, Gastropoda) in the Mediterranean Sea: Another Case of Cryptic Diversity. Diversity. 2022; 14(4):297. https://doi.org/10.3390/d14040297
Chicago/Turabian StyleFurfaro, Giulia, Christopher Schreier, Egidio Trainito, Miquel Pontes, Enric Madrenas, Pascal Girard, and Paolo Mariottini. 2022. "The Sea Slug Doriopsilla areolata Bergh, 1880 (Mollusca, Gastropoda) in the Mediterranean Sea: Another Case of Cryptic Diversity" Diversity 14, no. 4: 297. https://doi.org/10.3390/d14040297