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Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials

Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech 40000, Morocco
Agrobiosciences & Fertilizers Program, University Mohammed IV Polytechnic (UM6P), Benguerir 43150, Morocco
University Sultan Moulay Slimane, Polydisciplinary Faculty of Khouribga, BP. 145, Khouribga 25000, Morocco
Department of Biology, University of Waterloo, 200 University Avenue South, Waterloo, ON N2L 3G1, Canada
Laboratory of Ecology and Environment (L2E), Faculty of Science Semlalia, Cadi Ayyad University, Marrakesh 40000, Morocco
Author to whom correspondence should be addressed.
Diversity 2019, 11(8), 139;
Received: 22 June 2019 / Revised: 11 August 2019 / Accepted: 14 August 2019 / Published: 20 August 2019
The objectives of this study were to assess actinobacterial diversity in five Moroccan extreme habitats and to evaluate their plant growth-promoting (PGP) activities. The soil samples were collected from different locations, including soils contaminated with heavy metals, from a high altitude site, from the desert, and from a marine environment. In total, 23 actinobacteria were isolated, 8 from Merzouga sand soil; 5 from Cannabis sativa rhizospheric soil; 5 from Toubkal mountain; 4 from a Draa sfar mining site; and 1 from marine soil. Based on their genotypic classification using 16S rRNA gene sequences, 19 of all belonged to the genus Streptomyces (82%) while the rest are the members of the genera Nocardioides (4.5%), Saccharomonospora (4.5%), Actinomadura (4.5%), and Prauserella (4.5%). Isolates Streptomyces sp. TNC-1 and Streptomyces sp. MNC-1 showed the highest level of phosphorus solubilization activity with 12.39 and 8.56 mg/mL, respectively. All 23 isolates were able to solubilize potassium, and 91% of them could grow under nitrogen-free conditions. The ability of the isolated actinobacteria to form indole-3-acetic acid (IAA) ranged from 6.70 to 75.54 μg/mL with Streptomyces sp. MNC-1 being the best IAA producer. In addition, all of the actinobacteria could produce siderophores, with Saccharomonospora sp. LNS-1 synthesizing the greatest amount (138.92 μg/mL). Principal coordinate analysis revealed that Streptomyces spp. MNC-1, MNT-1, MNB-2, and KNC-5; Saccharomonospora sp. LNS-1; and Nocardioides sp. KNC-3 each showed a variety of high-level plant growth-promoting activities. The extreme environments in Morocco are rich with bioactive actinobacteria that possess a variety of plant growth-promoting potentials that can further benefit green and sustainable agriculture. View Full-Text
Keywords: actinobacteria; extremophile; plant growth-promoting bacteria actinobacteria; extremophile; plant growth-promoting bacteria
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MDPI and ACS Style

Nafis, A.; Raklami, A.; Bechtaoui, N.; El Khalloufi, F.; El Alaoui, A.; Glick, B.R.; Hafidi, M.; Kouisni, L.; Ouhdouch, Y.; Hassani, L. Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials. Diversity 2019, 11, 139.

AMA Style

Nafis A, Raklami A, Bechtaoui N, El Khalloufi F, El Alaoui A, Glick BR, Hafidi M, Kouisni L, Ouhdouch Y, Hassani L. Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials. Diversity. 2019; 11(8):139.

Chicago/Turabian Style

Nafis, Ahmed, Anas Raklami, Noura Bechtaoui, Fatima El Khalloufi, Abdelkhalek El Alaoui, Bernard R. Glick, Mohamed Hafidi, Lamfeddal Kouisni, Yedir Ouhdouch, and Lahcen Hassani. 2019. "Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials" Diversity 11, no. 8: 139.

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