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Open AccessArticle

Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials

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Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech 40000, Morocco
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Agrobiosciences & Fertilizers Program, University Mohammed IV Polytechnic (UM6P), Benguerir 43150, Morocco
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University Sultan Moulay Slimane, Polydisciplinary Faculty of Khouribga, BP. 145, Khouribga 25000, Morocco
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Department of Biology, University of Waterloo, 200 University Avenue South, Waterloo, ON N2L 3G1, Canada
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Laboratory of Ecology and Environment (L2E), Faculty of Science Semlalia, Cadi Ayyad University, Marrakesh 40000, Morocco
*
Author to whom correspondence should be addressed.
Diversity 2019, 11(8), 139; https://doi.org/10.3390/d11080139
Received: 22 June 2019 / Revised: 11 August 2019 / Accepted: 14 August 2019 / Published: 20 August 2019
The objectives of this study were to assess actinobacterial diversity in five Moroccan extreme habitats and to evaluate their plant growth-promoting (PGP) activities. The soil samples were collected from different locations, including soils contaminated with heavy metals, from a high altitude site, from the desert, and from a marine environment. In total, 23 actinobacteria were isolated, 8 from Merzouga sand soil; 5 from Cannabis sativa rhizospheric soil; 5 from Toubkal mountain; 4 from a Draa sfar mining site; and 1 from marine soil. Based on their genotypic classification using 16S rRNA gene sequences, 19 of all belonged to the genus Streptomyces (82%) while the rest are the members of the genera Nocardioides (4.5%), Saccharomonospora (4.5%), Actinomadura (4.5%), and Prauserella (4.5%). Isolates Streptomyces sp. TNC-1 and Streptomyces sp. MNC-1 showed the highest level of phosphorus solubilization activity with 12.39 and 8.56 mg/mL, respectively. All 23 isolates were able to solubilize potassium, and 91% of them could grow under nitrogen-free conditions. The ability of the isolated actinobacteria to form indole-3-acetic acid (IAA) ranged from 6.70 to 75.54 μg/mL with Streptomyces sp. MNC-1 being the best IAA producer. In addition, all of the actinobacteria could produce siderophores, with Saccharomonospora sp. LNS-1 synthesizing the greatest amount (138.92 μg/mL). Principal coordinate analysis revealed that Streptomyces spp. MNC-1, MNT-1, MNB-2, and KNC-5; Saccharomonospora sp. LNS-1; and Nocardioides sp. KNC-3 each showed a variety of high-level plant growth-promoting activities. The extreme environments in Morocco are rich with bioactive actinobacteria that possess a variety of plant growth-promoting potentials that can further benefit green and sustainable agriculture. View Full-Text
Keywords: actinobacteria; extremophile; plant growth-promoting bacteria actinobacteria; extremophile; plant growth-promoting bacteria
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Nafis, A.; Raklami, A.; Bechtaoui, N.; El Khalloufi, F.; El Alaoui, A.; Glick, B.R.; Hafidi, M.; Kouisni, L.; Ouhdouch, Y.; Hassani, L. Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials. Diversity 2019, 11, 139.

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