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Open AccessCommunication

Synthesis of (R) and (S)-3-Chloro-5-(2,4-dimethylpiperazin-1-yl)-4H-1,2,6-thiadiazin-4-ones

Department of Life Sciences, School of Sciences, European University Cyprus, 6 Diogenis Str., Engomi, P. O. Box 22006, 1516 Nicosia, Cyprus
Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Department of Chemistry, University of Cyprus, P. O. Box 20537, 1678 Nicosia, Cyprus
Author to whom correspondence should be addressed.
Molbank 2020, 2020(2), M1139;
Received: 4 May 2020 / Revised: 20 May 2020 / Accepted: 25 May 2020 / Published: 1 June 2020
(This article belongs to the Special Issue Heterocycle Reactions)


The reaction of 3,5-dichloro-4H-1,2,6-thiadiazin-4-one with (R) and (S)-1,3-dimethylpiperazines (1 equiv), in THF, at ca. 20 °C gives (R) and (S)-3-chloro-5-(2,4-dimethylpiperazin-1-yl)-4H-1,2,6-thiadiazin-4-ones in 70% and 68% yields, respectively. The new compounds were fully characterized.
Keywords: substitution; heterocycle; thiadiazine; piperazine; chirality substitution; heterocycle; thiadiazine; piperazine; chirality

1. Introduction

Piperazines are important saturated nitrogen containing heterocycles and appear in a number of clinically used pharmaceuticals [1]. Among the nitrogen-containing heterocycles, piperazines rank as third in the most frequently used U.S. FDA-approved drugs [2], while other uses include the production of polyamide plastics and in the capture of CO2 [3]. Examples of piperazine-containing drugs include the antibiotic ciprofloxacin, the erectile dysfunction drug sildenafil, and the anti-cancer BCR-ABL and src tyrosine kinase inhibitor bosutinib (SKI-606) [4] (Figure 1).
The incorporation of an asymmetric 3-methyl substituent to the piperazine moiety can improve the biological activity of a compound and enhance its physicochemical characteristics. There are many examples in the literature of 3-methylpiperazines exhibiting anti-cancer activity [5], or acting as antiplatelet agents [6], among others.
The introduction of 3-methylpiperazine in the design of kinase inhibitors offers another route to enhance potency on target and selectivity. While the main purpose of the added methyl group is to act as a steric handle to increase the torsion between adjacent ring systems on the solvent front of the ATP-binding pocket, it can also be used to probe a pocket in the active site. The 3-methylpiperazine has similar properties and uses to the 3-methylmorpholine substituent commonly used in kinase inhibitor design [7].
There are numerous examples of compounds where the 3-methylpiperazine substituent has had a pronounced effect on the overall profile of the compound (Figure 2) [5,8,9]. In the case of Talmapimod (SCIO-469), the incorporation of a 3-methylpiperazine into the structure helped reduce the metabolism of an adjacent benzyl group [8]. While in the case of a PAK4 inhibitor program that investigated compounds 1-3, the methyl group on the 3-methylpiperazine helped improve the compounds’ selectivity towards PAK4 over closely related PAK1 [5]. In a similar manner, the introduction of the gem-dimethylpiperazine moiety in a PI3K program (compound 4) enabled selectivity over family members for the PI3Kδ isoform [9,10].
The methyl group of the 3-methylpiperazine can also be used to probe narrow-band activity profiles in any medicinal chemistry program. This methyl scanning method was applied by Berlex Biosciences to screen an ADP receptor (P2Y12) antagonist hit [6]. The introduction of a methyl group is not always beneficial and the precise stereochemistry can be critical. While the steric effects can be achieved by other methods, the methyl group remains the most muted modification that provides substantial compound property improvements with a limited impact on ligand efficiency.
Our interest in the 1,3-dimethylpiperazine moiety is part of our ongoing effort to investigate the biological activity of novel 1,2,6-thiadiazines. Non-S-oxidized 1,2,6-thiadiazines are relatively unexplored heterocycles that have applications as organic photovoltaics (OPVs) [11], liquid crystals [12], plant protectants [13,14,15,16,17], and potential anticancer agents [18]. The chemistry of non-S-oxidized 1,2,6-thiadiazines has recently been reviewed [19]. Currently, we are developing a series of new 1,2,6-thiadiazine building blocks to expand our library of drug-like compounds with potential kinome selectivity profiles. For this work, we investigated the 3-methylmorpholine moiety as a substituent on 4H-1,2,6-thiadiazin-4-one [7]. In continuation of this work, we decided to investigate the 1,3-dimethylpiperazine moiety, which we planned to introduce by a selective nucleophilic displacement of one chloride of dichlorothiadiazinone 5 by (R) and (S)-1,3-dimethylpiperazine to yield 3-methylmorpholine-substituted thiadiazines 6a and 6b, respectively (Scheme 1). This displacement can occur under mild conditions owing to the electrophilic nature of the starting thiadiazine.

2. Results and Discussion

We reacted 3,5-dichloro-4H-1,2,6-thiadiazin-4-one (5) [20] with 1 equiv. of 1,3-dimethylpiperazines in anhydrous tetrahydrofuran (THF), at 20 °C. The dibasic nature of piperazines means that no extra base or excess of piperazine reagent is required. Dilution of the reaction mixture with dichloromethane (DCM) saturated in ammonia, followed by column chromatography, led to the isolation of the desired products 6a and 6b as yellow oils in 70% and 68% yields, respectively (Scheme 2, see Supplementary Materials for NMR spectra). Compared to the analogous 3-methylmorpholine derivatives [7], the 1H NMR spectra of the products 6a and 6b display increased line broadening, which can be attributed to decreased free rotation of the piperazine ring owing to a greater electron release into the thiadiazine. Similar hindered rotation phenomena of thiadiazines bound to secondary cyclic amines have been reported [21]. The optical rotation data showed that the two products were indeed enantiomers ([α ] D 20 +65 and −64, respectively, for 6a and 6b, see Materials and Methods).
We noted that the stereochemistry of the products 6a and 6b was attributed to the enantiomeric purity of the starting (R)- and (S)-1,3-dimethylpiperazines, [α ] D 20 +6.5 (c 1, CHCl3) and −6.0 (c 1, CHCl3), respectively. To the best of our knowledge, and in particular, under the mild reaction conditions used for the above nucleophilic substitutions, chiral 1,3-dimethylpiperazines do not epimerize.

3. Materials and Methods

The reaction mixture was monitored by thin layer chromatography (TLC) using commercial glass-backed TLC plates (Merck Kieselgel 60 F254). The plates were observed under UV light at 254 and 365 nm. Tetrahydrofuran (THF) was distilled over CaH2 before use. The UV-vis spectrum was obtained using a Perkin-Elmer Lambda-25 UV-vis spectrophotometer (Perkin-Elmer, Waltham, MA, USA) and inflections are identified by the abbreviation “inf”. Optical rotation was determined in a JASCO P-2000 polarimeter. The IR spectrum was recorded on a Shimadzu FTIR-NIR Prestige-21 spectrometer (Shimadzu, Kyoto, Japan) with the Pike Miracle Ge ATR accessory (Pike Miracle, Madison, WI, USA) and strong, medium and weak peaks are represented by s, m and w, respectively. 1H and 13C NMR spectra were recorded on a Bruker Avance 500 machine [at 500 and 125 MHz, respectively, (Bruker, Billerica, MA, USA)]. Deuterated solvents were used for homonuclear lock and the signals are referenced to the deuterated solvent peaks. Attached proton test (APT) NMR studies were used for the assignment of the 13C peaks as CH3, CH2, CH, and Cq (quaternary). The MALDI-TOF mass spectrum (+ve mode) was recorded on a Bruker Autoflex III Smartbeam instrument (Bruker). 3,5-Dichloro-4H-1,2,6-thiadiazin-4-one (5) was prepared according to the literature procedure [20,22].
(R)-3-Chloro-5-(2,4-dimethylpiperazin-1-yl)-4H-1,2,6-thiadiazin-4-one (6a)
To a stirred mixture of 3,5-dichloro-4H-1,2,6-thiadiazin-4-one (5) (91.5 mg, 0.500 mmol) in THF (1 mL) at ca. 20 °C, was added in one portion (R)-1,3-dimethylpiperazine (57.0 mg, 0.500 mmol). The mixture was protected with a CaCl2 drying tube and stirred at this temperature until complete consumption of the starting material (TLC, 24 h). DCM saturated with NH3 (10 mL) was then added, the mixture adsorbed onto silica and chromatography (DCM/t-BuOMe 50:50) gave the title compound 6a (91.7 mg, 70%) as a yellow oil; Rf 0.48 (DCM/t-BuOMe, 50:50); [α ] D 20 +65 (c 1.0, CHCl3); (found: C, 41.57; H, 4.93; N, 21.46. C9H13ClN4OS requires C, 41.46; H, 5.03; N, 21.49%); λmax(DCM)/nm 269 (log ε 3.13), 313 (3.36), 322 (3.34), 410 (2.96); vmax/cm−1 2970w, 2941w, 2843w and 2793w (C-H), 1630s, 1495s, 1462m, 1433m, 1404w, 1383w, 1339w, 1323w, 1298m, 1281m, 1229m, 1194m, 1169w, 1146m, 1096w, 1076w, 1049m, 997w, 978w, 964w, 939w, 918w, 903m, 891m, 870m, 854m, 845m, 800m, 725m; δH(500 MHz; CDCl3) 4.98 (1H, br s, CHN), 4.61 (1H, br s, CHN), 3.41 (1H, dd, J 11.1, 11.1, CHN), 2.98 (1H, br s, CHN), 2.82 (1H, d, J 9.2, CHN), 2.37 (4H, br s, CHN & NCH3), 2.25 (1H, br s, CHN), 1.42 (3H, d, J 6.6, CHCH3); δC(125 MHz; CDCl3) 158.7 (Cq), 152.5 (Cq), 145.2 (Cq), 59.2 (CH2N), 54.8 (CH2N), 49.0 (CHN), 45.7 (NCH3), 40.5 (CH2N), 16.1 (CHCH3); m/z (MALDI-TOF) 263 (MH++2, 31%), 261 (MH+, 77), 177 (100), 142 (21), 113 (37).
(S)-3-Chloro-5-(2,4-dimethylpiperazin-1-yl)-4H-1,2,6-thiadiazin-4-one (6b)
To a stirred mixture of 3,5-dichloro-4H-1,2,6-thiadiazin-4-one (5) (91.5 mg, 0.500 mmol) in THF (1 mL) at ca. 20 °C, was added in one portion (S)-1,3-dimethylpiperazine (57.0 mg, 0.500 mmol). The mixture was protected with a CaCl2 drying tube and stirred at this temperature until complete consumption of the starting material (TLC, 24 h). DCM saturated with NH3 (10 mL) was then added, the mixture adsorbed onto silica and chromatography (DCM/t-BuOMe 50:50) gave the title compound 6b (89.2 mg, 68%) as a yellow oil; Rf 0.48 (DCM/t-BuOMe, 50:50); [α ] D 20 −64 (c 1.0, CHCl3); (found: C, 41.52; H, 4.88; N, 21.33. C9H13ClN4OS requires C, 41.46; H, 5.03; N, 21.49%); λmax(DCM)/nm 270 (log ε 3.13), 313 (3.35), 321 (3.33), 410 (2.96); vmax/cm−1 2974w, 2940w, 2845w and 2795w (C-H), 1630s, 1495s, 1462m, 1433m, 1404w, 1383w, 1339w, 1323w, 1298m, 1281m, 1229m, 1194m, 1169w, 1146m, 1096w, 1076w, 1049m, 997w, 978w, 963w, 939w, 918w, 903m, 893m, 870m, 854m, 845m, 802m, 727m; δH(500 MHz; CDCl3) 4.97 (1H, br s, CHN), 4.60 (1H, br s, CHN), 3.38 (1H, ddd, J 13.0, 13.0, 2.7, CHN), 2.95 (1H, d, J 11.0, CHN), 2.77 (1H, d, J 11.4, CHN), 2.38 (4H, br s, CHN & NCH3), 2.21 (1H, dd, J 11.2, 11.2, CHN), 1.40 (3H, d, J 6.8, CHCH3); δC(125 MHz; CDCl3) 158.7 (Cq), 152.5 (Cq), 145.1 (Cq), 59.4 (CH2N), 54.9 (CH2N), 49.1 (CHN), 46.0 (NCH3), 40.6 (CH2N), 16.0 (CHCH3); m/z (MALDI-TOF) 263 (MH++2, 29%), 261 (MH+, 100), 225 (30), 142 (50), 112 (10).

4. Conclusions

(R) and (S)-3-chloro-5-(2,4-dimethylpiperazin-1-yl)-4H-1,2,6-thiadiazin-4-ones were prepared in good yields from 3,5-dichloro-4H-1,2,6-thiadiazin-4-one. These compounds can be of interest to the medicinal and materials science sectors, this work provides a valuable route to these intermediates. The chemistry of these two aminothiadiazines will be further investigated to assess their potential applications.

Supplementary Materials

The following are available online, mol file, 1H and 13C NMR spectra.

Author Contributions

P.A.K., C.R.M.A. and A.S.K. conceived the experiments; A.S.K. designed and performed the experiments, analyzed the data and wrote the paper; P.A.K. and C.R.M.A. edited the paper. All authors have read and agreed to the published version of the manuscript.


This research was funded by the Cyprus Research Promotion Foundation (Grants: ΣΤΡAΤHΙΙ/0308/06, NEKYP/0308/02 ΥΓΕΙA/0506/19 and ΕΝΙΣΧ/0308/83).


The authors thank the following organizations and companies in Cyprus for generous donations of chemicals and glassware: the State General Laboratory, the Agricultural Research Institute, the Ministry of Agriculture, MedoChemie Ltd., Medisell Ltd. and Biotronics Ltd. Furthermore, we thank the A. G. Leventis Foundation for helping to establish the NMR facility at the University of Cyprus.

Conflicts of Interest

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.


  1. James, T.; MacLellan, P.; Burslem, G.M.; Simpson, I.; Grant, J.A.; Warriner, S.; Sridharan, V.; Nelson, A. A modular lead-oriented synthesis of diverse piperazine, 1,4-diazepane and 1,5-diazocane scaffolds. Org. Biomol. Chem. 2014, 12, 2584–2591. [Google Scholar] [CrossRef] [PubMed]
  2. Vitaku, E.; Smith, D.T.; Njardarson, J.T. Analysis of the Structural Diversity, Substitution Patterns, and Frequency of Nitrogen Heterocycles among U.S. FDA Approved Pharmaceuticals. J. Med. Chem. 2014, 57, 10257–10274. [Google Scholar] [CrossRef] [PubMed]
  3. Rochelle, G.; Chen, E.; Freeman, S.; Van Wagener, D.; Xu, Q.; Voice, A. Aqueous piperazine as the new standard for CO2 capture technology. Chem. Eng. 2011, 171, 725–733. [Google Scholar] [CrossRef]
  4. Boschelli, D.H.; Ye, F.; Wang, Y.D.; Dutia, M.; Johnson, S.L.; Wu, B.; Miller, K.; Powell, D.W.; Yaczko, D.; Young, M.; et al. Optimization of 4-phenylamino-3-quinolinecarbonitriles as potent inhibitors of Src kinase activity. J. Med. Chem. 2001, 44, 3965–3977. [Google Scholar] [CrossRef] [PubMed]
  5. Hao, C.; Zhao, F.; Song, H.; Guo, J.; Li, X.; Jiang, X.; Huan, R.; Song, S.; Zhang, Q.; Wang, R.; et al. Structure-based design of 6-chloro-4-aminoquinazoline-2-carboxamide derivatives as potent and selective p21-activated kinase 4 (PAK4) ιinhibitors. J. Med. Chem. 2018, 61, 265–285. [Google Scholar] [CrossRef] [PubMed]
  6. Islam, I.; Yuan, S.; Wei, R.G.; Xu, W.; Morrissey, M.; Mohan, R.; Zheng, D.; DiMella, A.; Dunning, L.; Snider, M.; et al. Reversible, orally available ADP receptor (P2Y12) antagonists part I: Hit to lead process. Bioorg. Med. Chem. Lett. 2018, 28, 1459–1463. [Google Scholar] [CrossRef] [PubMed]
  7. Kalogirou, A.S.; Asquith, C.R.M.; Koutentis, P.A. Synthesis of (R) and (S)-3-Chloro-5-(3-methylmorpholino)-4H-1,2,6-thiadiazin-4-ones. Molbank 2020, 2020, M1128. [Google Scholar] [CrossRef]
  8. Tan, X.; Tester, R.W.; Luedtke, G.R.; Chakravarty, S.; Mavunkel, B.J.; Perumattam, J.J.; Lu, Q.; Nashashibi, I.; Jung, J.; Hu, J.; et al. Design and synthesis of piperazine-indole p38 alpha MAP kinase inhibitors with improved pharmacokinetic profiles. Bioorg. Med. Chem. Lett. 2010, 20, 828–831. [Google Scholar] [CrossRef] [PubMed]
  9. Safina, B.S.; Baker, S.; Baumgardner, M.; Blaney, P.M.; Chan, B.K.; Chen, Y.-H.; Cartwright, M.W.; Castanedo, G.; Chabot, C.; Cheguillaume, A.J.; et al. Discovery of novel PI3-kinase δ specific inhibitors for the treatment of rheumatoid arthritis: Taming CYP3A4 time-dependent inhibition. J. Med. Chem. 2012, 55, 5887–5900. [Google Scholar] [CrossRef] [PubMed]
  10. Talele, T.T. Natural-Products-Inspired Use of the gem-Dimethyl Group in Medicinal Chemistry. J. Med. Chem. 2018, 61, 2166–2210. [Google Scholar] [CrossRef] [PubMed]
  11. Chochos, C.L.; Kalogirou, A.S.; Ye, T.; Tatsi, E.; Katsouras, A.; Zissimou, G.A.; Gregoriou, V.G.; Avgeropoulos, A.; Koutentis, P.A. 4H-1,2,6-Thiadiazine-containing donor–acceptor conjugated polymers: Synthesis, optoelectronic characterization and their use in organic solar cells. J. Mater. Chem. C 2018, 6, 3658–3667. [Google Scholar] [CrossRef]
  12. Gómez, T.; Macho, S.; Miguel, D.; Neo, A.G.; Rodríguez, T.; Torroba, T. Cyclopentathiadiazines, cyclohepta- and cyclopentadithiazoles: New materials and a rich heterocyclic chemistry of cyclic enaminonitriles. Eur. J. Org. Chem. 2005, 2005, 5055–5066. [Google Scholar] [CrossRef]
  13. Peake, C.J.; Harnish, W.N.; Davidson, B.L. Mono-5-substituted-3-chloro-4H-1,2,6-thiadiazin-4-one antifungal agents. U.S. Patent 4,097,594A, 27 June 1978. [Google Scholar]
  14. Peake, C.J.; Harnish, W.N.; Davidson, B.L. Mono-5-substituted-thio-3-chloro-4H-1,2,6-thiadiazin-4-one antifungal agents. U.S. Patent 4,100,281A, 27 June 1978. [Google Scholar]
  15. Peake, C.J.; Harnish, W.N.; Davidson, B.L. 3-Chloro-5-(optionally substituted heterocycloxy)-4H-1,2,6-thiadiazin-4-one antifungal agents. U.S. Patent 4,143,138, 3 March 1979. [Google Scholar]
  16. Peake, C.J.; Harnish, W.N.; Davidson, B.L. Mono-5-substituted-3-chloro-4H-1,2,6-thiadiazin-4-one antifungal agents. U.S. Patent 4,201,780, 6 May 1980. [Google Scholar]
  17. Portnoy, R.C. Thiadiazinone plant disease control agents. U.S. Patent 4,497,807A, 5 February 1985. [Google Scholar]
  18. Asquith, C.R.M.; Godoi, P.H.; Couñago, R.M.; Laitinen, T.; Scott, J.W.; Langendorf, C.G.; Oakhill, J.S.; Drewry, D.H.; Zuercher, W.J.; Koutentis, P.A.; et al. 1,2,6-Thiadiazinones as Novel Narrow Spectrum Calcium/Calmodulin-Dependent Protein Kinase Kinase 2 (CaMKK2) Inhibitors. Molecules 2018, 23, 1221. [Google Scholar] [CrossRef] [PubMed]
  19. Kalogirou, A.S.; Koutentis, P.A. The chemistry of non-S-oxidised 4H-1,2,6-thiadiazines. Targets Heterocycl. Syst. 2018, 22, 82–118. [Google Scholar] [CrossRef]
  20. Geevers, J.; Trompen, W.P. Synthesis and reactions of 3,5-dichloro-4H-1,2,6-thiadiazin-4-one. Recl. Trav. Chim. Pays Bas 1974, 93, 270–272. [Google Scholar] [CrossRef]
  21. Koutentis, P.A.; Rees, C.W. Reaction of tetracyanoethylene with SCl2; new molecular rearrangements. J. Chem. Soc. Perkin Trans. 2000, 1, 1089–1094. [Google Scholar] [CrossRef]
  22. Kalogirou, A.S.; Koutentis, P.A. A qualitative comparison of the reactivities of 3,4,4,5-tetrachloro-4H-1,2,6-thiadiazine and 4,5-dichloro-1,2,3-dithiazolium chloride. Molecules 2015, 20, 14576–14594. [Google Scholar] [CrossRef] [PubMed]
Figure 1. Piperazine-containing drugs.
Figure 1. Piperazine-containing drugs.
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Figure 2. 3-Methylpiperazine-containing pre-clinical kinase inhibitors.
Figure 2. 3-Methylpiperazine-containing pre-clinical kinase inhibitors.
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Scheme 1. Planned synthesis of 1,3-dimethylpiperazine-substituted thiadiazines 6a and 6b.
Scheme 1. Planned synthesis of 1,3-dimethylpiperazine-substituted thiadiazines 6a and 6b.
Molbank 2020 m1139 sch001
Scheme 2. Synthesis of (R) and (S)-3-chloro-5-(2,4-dimethylpiperazin-1-yl)-4H-1,2,6-thiadiazin-4-one 6a and 6b.
Scheme 2. Synthesis of (R) and (S)-3-chloro-5-(2,4-dimethylpiperazin-1-yl)-4H-1,2,6-thiadiazin-4-one 6a and 6b.
Molbank 2020 m1139 sch002
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