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Article

Interferon Receptor Chain Deficiency in Murine Friend Erythroleukemia Cell Clone Resistant to Type I or Type I and II Interferons

by
Zulema Antonia Percario
1,†,
Giorgio Mangino
2,†,
Arianna Raponi
1,
Emiliano Fratini
3,
Gabriele Vaccari
4,
Flavia Giannessi
1,
Gianna Fiorucci
5,
Manuela Cervelli
1,
Giovanna Romeo
2 and
Elisabetta Affabris
1,*
1
Department of Science, Roma Tre University, 00146 Rome, Italy
2
Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, Italy
3
Laboratory of Red Biotechnologies, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Casaccia Research Centre, 00123 Rome, Italy
4
Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, 00161 Rome, Italy
5
Institute of Molecular Biology and Pathology, Consiglio Nazionale delle Ricerche, 00185 Rome, Italy
*
Author to whom correspondence should be addressed.
†
These authors equally contributed to this work and shared first authorship.
Int. J. Mol. Sci. 2026, 27(13), 5908; https://doi.org/10.3390/ijms27135908
Submission received: 5 March 2026 / Revised: 13 June 2026 / Accepted: 15 June 2026 / Published: 30 June 2026
(This article belongs to the Section Molecular Microbiology)
Review Reports Versions Notes

Abstract

Interferon (IFN)-resistant cell clones 3Cl8 and 3γR8, isolated from wild-type Friend erythroleukemia cells 745A were characterized to identify the resistance defect. The 3Cl8 cell clone is resistant to type I IFNs and sensitive to type II IFN, whereas 3γR8, derived from 3Cl8, is resistant to both type I and II IFNs. Here, we report that no activation of the JAK-STAT pathway is detected after IFN treatment of resistant cells. Interestingly, the absence of major transcripts of the IFNAR2 receptor chain has been observed in type I IFN-resistant cells, and a point mutation relative to the IFNGR2 receptor chain (β chain) has been identified in type II IFN-resistant cells, inducing a frameshift leading to premature termination of translation. In addition, we have identified a new polymorphism of the murine IFNAR1 chain and possibly the presence of a murine IFNAR2b transmembrane, non-transducing chain in 745A cells, similar to that observed in humans and differing from previous reports on other murine systems.
Keywords: type I IFN; type II IFN; type I IFN receptors; type II IFN receptor; murine IFNAR; murine IFNGR; Friend erythroleukemia cells; murine retrovirus; Friend erythroleukemia virus type I IFN; type II IFN; type I IFN receptors; type II IFN receptor; murine IFNAR; murine IFNGR; Friend erythroleukemia cells; murine retrovirus; Friend erythroleukemia virus

Share and Cite

MDPI and ACS Style

Percario, Z.A.; Mangino, G.; Raponi, A.; Fratini, E.; Vaccari, G.; Giannessi, F.; Fiorucci, G.; Cervelli, M.; Romeo, G.; Affabris, E. Interferon Receptor Chain Deficiency in Murine Friend Erythroleukemia Cell Clone Resistant to Type I or Type I and II Interferons. Int. J. Mol. Sci. 2026, 27, 5908. https://doi.org/10.3390/ijms27135908

AMA Style

Percario ZA, Mangino G, Raponi A, Fratini E, Vaccari G, Giannessi F, Fiorucci G, Cervelli M, Romeo G, Affabris E. Interferon Receptor Chain Deficiency in Murine Friend Erythroleukemia Cell Clone Resistant to Type I or Type I and II Interferons. International Journal of Molecular Sciences. 2026; 27(13):5908. https://doi.org/10.3390/ijms27135908

Chicago/Turabian Style

Percario, Zulema Antonia, Giorgio Mangino, Arianna Raponi, Emiliano Fratini, Gabriele Vaccari, Flavia Giannessi, Gianna Fiorucci, Manuela Cervelli, Giovanna Romeo, and Elisabetta Affabris. 2026. "Interferon Receptor Chain Deficiency in Murine Friend Erythroleukemia Cell Clone Resistant to Type I or Type I and II Interferons" International Journal of Molecular Sciences 27, no. 13: 5908. https://doi.org/10.3390/ijms27135908

APA Style

Percario, Z. A., Mangino, G., Raponi, A., Fratini, E., Vaccari, G., Giannessi, F., Fiorucci, G., Cervelli, M., Romeo, G., & Affabris, E. (2026). Interferon Receptor Chain Deficiency in Murine Friend Erythroleukemia Cell Clone Resistant to Type I or Type I and II Interferons. International Journal of Molecular Sciences, 27(13), 5908. https://doi.org/10.3390/ijms27135908

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