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International Journal of Molecular Sciences
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2 April 2025

Investigation of the Possible Antibacterial Effects of Corticioid Fungi Against Different Bacterial Species

,
,
and
1
Institute of Forest Sciences, Białystok University of Technology, Wiejska St. 45E, 15-351 Białystok, Poland
2
Department of Agri-Food Engineering and Environmental Management, Białystok University of Technology, Wiejska St. 45E, 15-351 Białystok, Poland
3
NatureTECH Centre of Natural Products Research, Białystok University of Technology, Wiejska St. 45E, 15-351 Białystok, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci.2025, 26(7), 3292;https://doi.org/10.3390/ijms26073292
This article belongs to the Special Issue Novel Antimicrobial Agents
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Abstract

Extracts from 58 species of corticioid fungi (phylum Basidiomycota), mainly belonging to the orders Hymenochaetales, Polyporales and Russulales, were tested for their inhibitory activity against five species of bacteria: Corynebacterium striatum, Haemophilus influenzae, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Twenty-four of the species we analyzed in this study were tested for antibacterial activity for the first time. The fruiting bodies of the fungi were collected from dead wood in the forests of north-eastern Poland, and macerated in methanol. Dried extracts were redissolved in dimethyl sulfoxide and applied to broth cultures of the bacteria, which were then inoculated on agar plates. Noblesia crocea demonstrated moderate inhibitory activity against all five tested bacteria; Amylocorticium subincarnatum, Laxitextum bicolor, Peniophora laeta, P. rufomarginata, Phanerochaete sordida, and Xylobolus frustulatus inhibited four bacterial species. The extracts from 14 fungal species tested were moderately active against only two bacteria, P. aeruginosa and C. striatum; 17 species were active against C. striatum only. The full inhibition was observed with concentrations of extract 25 or 50 mg/mL.

1. Introduction

Bacterial pathogens pose a significant threat to human health. The increasing prevalence of antibiotic-resistant strains underscores the urgent need for novel therapeutic strategies [,,,]. Research is increasingly focused on exploring solutions of natural origin to combat these formidable pathogens []. Some of the most serious bacteria include Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Haemophilus influenzae, and Corynebacterium striatum. They are notable for their association with serious respiratory infections, which are challenging to manage due to their ability to form biofilms and develop resistance to antibiotics [,,,].
The bacteria listed above can cause not only respiratory diseases, but also other health issues. Staphylococcus aureus is a Gram-positive opportunistic prokaryote that is a significant component of the respiratory microbiota []. It is a major agent of pneumonia, but can also cause sinusitis [], skin and cardiovascular infections, sepsis, and nosocomial bacteremia []. Klebsiella pneumoniae is a Gram-negative bacterium that occurs naturally in the human digestive tract. In recent years, it has emerged as an important pathogen due to the increasing occurrence of hypervirulent and antibiotic-resistant strains. It causes infections of the lungs, urinary tract, bloodstream, wound or surgical site, and brain []. Pseudomonas aeruginosa is a Gram-negative opportunistic prokaryote found in humans mainly in the gastrointestinal tract or on the skin. It is a major component of the respiratory microbiota [] and causes pneumonia, bacteremia, urinary tract and surgical infections []. Haemophilus influenzae is a Gram-negative bacterium that is transmitted by airborne droplets. It can cause sinusitis, pharyngitis [], meningitis, otitis media, and septicemia []. Corynebacterium striatum is a Gram-positive, multi-drug resistant prokaryote that can cause nosocomial outbreaks. It is a part of the normal skin microbiota but can provoke bacteremia, endocarditis, and pneumonia under the right conditions [,].
The epidemiological challenges of nowadays, along with the fairly fast development of the resistance of bacteria to commercial antibiotics [,,,], is the cause for searching for new antibacterial substances. Fungi are considered to be a source of many as yet undiscovered antibacterial compounds for potential future use [,]. There is a prospect that some of the new antibacterial substances will not only be useful in treating bacterial infections. This is based on the fact that some substances known as antibacterial drugs have antiviral activity against SARS-CoV-2 and an immunomodulatory effect [,]. Deadwood-associated fungi have shown remarkable potential in the discovery of bioactive compounds with medicinal properties. These fungi are known to produce secondary metabolites with antimicrobial, antiviral, and anticancer activities, making them a valuable resource for drug development. Research into these fungi could provide solutions to pressing challenges such as antibiotic resistance.
Among Basidiomycota, corticioid fungi are an important group of wood-decaying organisms, found in all wood-containing ecosystems on Earth. They are particularly common on fallen wood, both small-sized and coarse, in temperate and boreal forests, and eventually participate in soil formation. Morphologically corticioid fungi are characterized by flat fruiting bodies (basidiomata), usually 0.05–0.5 mm thick and effused over the substratum, and bearing one-celled basidia []. However, in terms of natural classification, they belong to different orders of Agaricomycetes, with a few exceptions of the genera assigned to the classes Dacrymycetes and Tremellomycetes [,]. Wood-inhabiting Basidiomycota, including the corticioid fungi, compete with bacteria for low molecular weight nutrients in dead wood []. It has been demonstrated experimentally that the wood colonized by some corticioid fungi contains significantly fewer bacterial cells, than the wood without these fungi, as in the case of Phlebiopsis gigantea [] and Resinicium bicolor []. Consequently, it is recognized that corticioid fungi can produce antibacterial compounds. Some of these substances have been isolated from the genera Dentipellis, Merulius, Peniophora, Stereum, and Xylobolus [,,,,,,]. However, none of these compounds has yet been commercialized for medical application, and their mechanisms of action on the bacterial cell remain unknown.
There have been a number of pioneering papers on the antibacterial properties of extracts from the fruiting bodies of wild basidiomycetes, including three that were the first to study corticioid fungi. Wilking and Harris [] screened 37 corticioid fungi, of which 9 species only demonstrated antibacterial properties. In the study by Mathieson [], 13 species of corticoid fungi were tested and 3 of them showed antibacterial activity. Wilkins [] studied 8 corticioid species, 2 of which were active against bacteria. In these studies, the authors obtained extracts from ground basidiocarps, with or without the addition of water, and applied them to the agar plates inoculated with bacteria. This type of experiment was considered to be a preliminary test, whereas the tests with fungi isolated in pure culture were more substantial.
In subsequent work, only basidiomata of sufficient thickness, length, and ease of removal from the substratum were used for antibacterial studies. Gianetti et al. [] isolated the antibacterial substances: merulinic acids A, B, and C from the fruiting bodies of Merulius tremellosus and Phlebia radiata. Zjawiony et al. [] proved that the ethanolic extract of the fruiting bodies of Byssomerulius incarnatus has antibacterial properties against S. aureus. Cateni et al. [] extracted by methanol and purified four compounds from fresh Stereum hirsutum basidiomata, and reported the activity of compounds from this fungus against Mycobacterium tuberculosis. Ferreira-Silva et al. [] found that ethyl acetate extract from Stereum ostrea basidiomata was active against S. aureus. Tamrakar et al. [] found the antibacterial activity of ethanolic extracts from fruiting bodies of Phlebia tremellosa and Xylobolus princeps. Sevindik et al. [] showed that ethanolic and methanolic extracts from fruiting bodies of Stereum hirsutum were active against five species of bacteria. İnci et al. [] found that ethanolic extract from Hymenochaete rubiginosa basidiomata was active against five bacterial strains. For most species of corticioid fungi, which have thin basidiomata closely adnate to the wooden substratum, no antibacterial tests have been attempted since 1946.
In addition, a number of papers have been published after studying the antibacterial activities of pure cultures of corticioid fungi. In these cases, the live fungi or their derivatives were applied against bacteria in vitro. The mycelium of corticioid fungi growing on agar or agar fragments taken from the surrounding of the growing fungi have been used in screening studies [,,,,,,]. The broth from the submerged culture of the fungi was used in the works [,,]. Methanolic extracts of the whole submerged culture of the fungi were tested by Suay et al. []. Extracts from mycelia obtained after submerged cultivation were used by Grey et al. [] and Rosa et al. []. Methanolic extracts from mycelium after solid-state cultivation were tested by Zrimec et al. [].
The aim of this study was to screen the crude extracts of corticioid fungi, including 24 species for the first time, for antibacterial properties against the main bacteria known as agents of respiratory co-infections.

2. Results

The crude extracts involved in the research were yellow-brown or dark brown in color, with specific variations ranging from brownish-yellow in Hyphoderma setigerum to reddish-black in Noblesia crocea and almost pure black in Boreostereum radiatum. The consistency of the extracts was that of a soft resin or soft paste. The extraction yield depended on the ratio of the dry mass of the fungus to the volume of methanol, with significant differences between small and large weight samples. For samples from 0.6 to 3.0 g the yield was 8.8–60.4% with an average of 24.3%. For fungal samples from 3.1 to 75.0 g the yield ranged from 2.2 to 45.0% with an average of 11.4%. In the latter group, the highest yield was for Byssomerulius corium (27.9%), Dentipellis fragilis (28.2%), and Noblesia crocea (45.0%). Due to the variation in dry mass of the samples, size, and density of the basidiomata pieces, it was not realistic to use identical sample/solvent ratios (w/w or v/v) for all samples. In this respect, it was observed that the efficiency of extraction increased significantly with the decreasing material volume and increasing solvent volume up to 1:10 and higher.
Of the 58 fungal species tested, extracts from 51 species (88%) demonstrated total inhibition (at very high concentrations) of at least one bacterial strain (Table 1). No inhibition of bacterial growth was observed in solvent controls (DMSO).
Table 1. Inhibitory activity of corticioid fungi extracts against selected bacteria.
The activity of 24 species of corticioid fungi against bacteria was tested for the first time. We also screened 58 fungal species for the first time for activity against H. influenzae and C. striatum.
Amylocorticium subincarnatum, Laxitextum bicolor, Peniophora laeta, P. rufomarginata, Phanerochaete sordida, and Xylobolus frustulatus moderately inhibited four bacterial strains. Fourteen corticioid species tested were only active against two bacteria: P. aeruginosa and C. striatum; 17 fungal species were only active against C. striatum; 7 species had no activity against bacteria in this assay.
Notable differences in activity between samples were observed in 12 species (Table 1). In Phlebiopsis gigantea, the winter collected sample with older basidiomata (No. 247-1) was more active, than the spring one with younger basidiomata (No. 247-2). In Byssomerulius corium, the spring collected sample with older basidiomata (No. 264) was active, whereas the autumn sample with younger basidiomata (No. 245) was inactive. In Xylobolus frustulatus, the summer sample (No. 186-1) was more active than the spring one (No. 186-2). In Resinicium bicolor, the autumn collection (No. 308) was more active than spring one (No. 276). In Peniophora rufomarginata, the autumn sample (No. 249-2) was more active, than the winter one (No. 249-1). In Baltazaria galactina, the autumn sample (No. 233) was active, whereas the summer one (No. 231) was inactive.
For some samples, the influence of the substratum was also admitted. For example, Baltazaria galactina was active in the case of the sample from Tilia cordata (No. 233) and inactive in the sample from Populus tremula (No. 231). Byssomerulius corium collected from Carpinus betulus (No. 264) showed moderate activity, but that collected from Populus tremula (No. 245) was inactive. Resinicium bicolor collected from Pinus sylvestris (No. 308) was active, but that one collected from Picea abies (No. 276) was inactive. For Lyomyces crustosus only, no variation in activity between samples was detected.
The distribution of activities among the members of one genus was, according to our results, rather uneven. For example, the activity was not detected in Amylocorticium cebennense, whereas it was remarkable in A. subincarnatum. A similar contrast is observed between Phanerochaete velutina and Ph. sordida, Peniophora quercina and P. rufomarginata. For the genera Hymenochaete, Hyphoderma, Stereum, and Xylodon the patterns of moderate activity are similar for species within a genus.
The sample collection was focused on three orders of the fungi, which allows for tracing a distribution pattern of antibacterial activities at the order level (Figure 1). Taking into account the number of sensitive bacterial strains, moderate screening results belonged to the Polyporales and the Russulales. The activities of the Hymenochaetales species were limited to less sensitive bacterial strains.
Figure 1. Distribution of antibacterial activity according to fungal order.
A clear pattern of differential susceptibility of bacterial strains to the fungal metabolites can be seen in the present study. This can be described through the number of fungal species that moderately inhibit the growth of each strain of bacteria (Figure 2). Namely, C. striatum and P. aeruginosa were found to be more susceptible, whereas S. aureus, K. pneumoniae, and H. influenzae were more resistant. No dependence of susceptibility on the type of fungal cell wall (Gram-positive or Gram-negative) was observed in our study.
Figure 2. Susceptibility of the bacterial strains—the number of fungal species for which inhibition of the strains by at least one of the samples tested was observed. Names of the Gram-positive strains are underlined in blue and the Gram-negative strains in pink.

3. Discussion

This study demonstrated that fungal metabolites have a moderate effect on different bacteria than would be expected based on the microbiology of their habitats. This is evident from the fact that human pathogenic bacteria from the list of strains tested above are not known from dead wood in forests. However, studies by molecular methods have shown that the genus Pseudomonas is common in dead wood [,,]. In addition, a Staphylococcus species related to S. aureus was recorded in dead wood []. Thus, fungi possess bactericidal mechanisms not only against their direct competitors but also against relatives of such competitive bacteria.
Although the activity of the tested fungi was not so sufficient to make them promising as antibiotic agents, our study showed that the percentage of more active species was higher in the orders Polyporales and Russulales. We assume that in the Russulales, such activities are associated with the presence of gloeocystidia as a receptacle of secondary metabolites, like in Laxitextum, Peniophora, and Xylobolus, whereas in the Polyporales, the association of active substances with the anatomical structures of the basidioma has not yet been hypothesized.
A remarkable phenomenon is that the activity against a bacterial strain is not repeated for different samples belonging to the same fungal species. This inconsistency has already been noted in a pioneer work [] for fruiting bodies collected in different years and different locations. The activity was also determined to be strain-dependent in tests with fungi isolated in pure culture [,,].
Apart from the ontogenesis of the vegetative body, individual fruiting bodies of corticioid fungi have their developmental stages from primordia to the collapse of hymenial elements and total destruction of the basidioma. Using younger and older basidiomata for extraction we consider as a cause of different antibacterial activities manifestation between samples of the same species. Different ontogenetic stages of the samples were also a supposed reason for the different colors of crude extracts for the same species. Furthermore, we assume that different proportions of sample/solvent weight during maceration can be a cause of differences in active substance concentration between samples. No apparent pattern of activity dependence on the season of fruitbody collection was observed, but a larger number of samples of individual species from the wild are required for reasoned conclusions.
Eighteen species from our study were previously tested for activity against S. aureus in three pioneering papers, based on basidiomata collected in situ [,,]. In these experiments, extracts were prepared as fungal homogenate in distilled water 1:1 w/w [], or the liquid, naturally present in the fresh fruiting body, was squeezed out [,]. However, for the second case, the articles do not give details of how the liquid was obtained for basidiomata thinner than 0.5 mm. Overall, only two fungal species from our study showed moderate activity against S. aureus, as in these publications: Byssomerulius corium [] and Phlebiopsis gigantea []. The activity against S. aureus reported for Hyphoderma setigerum, Stereum sanguinolentum [], Phlebia rufa, and Scopuloides hydnoides [] was not confirmed in our research. Contrary to our results, the above authors did not report activity against S. aureus in Chondrostereum purpureum and Peniophora cinerea.
Screening for antimicrobial properties of fungi using the material from in situ-developed fruiting bodies can be a first step before trials with their pure cultures. At the same time, we acknowledge that wild-growing basidiomata may contain a complex of substances with antibacterial activity that is not produced in the same quality and quantity as mycelium grown in vitro.
We, therefore, compared our results with those of tests against S. aureus using pure living cultures of corticioid fungi [,]. Three summaries can be distinguished as follows:
(1)
The fungal species that inhibited the growth of S. aureus both in our experiments and as mycelia growing in culture are the following: Baltazaria galactina, Byssomerulius corium, Chondrostereum purpureum, Laxitextum bicolor, Noblesia crocea, Peniophora cinerea, and Xylobolus frustulatus;
(2)
The fungal species that inhibited the growth of S. aureus as fruiting body extracts, but did not show such activity as mycelia in culture are the following: Phanerochaete sordida and Phlebiopsis gigantea.
(3)
The fungal species that were not active in their fruiting body extracts but were active as living mycelia are the following: Hydnoporia tabacina, Hymenochaete rubiginosa, Mycoacia livida, Peniophora incarnata, P. quercina, Stereum hirsutum, S. rugosum, and S. sanguinolentum.
The same comparison was conducted with published data on the inhibitory activity of extracts from cultures against S. aureus [,]. In this case, two groups of species can be distinguished as follows:
(1)
The fungi that inhibited S. aureus as fruitbody extracts, but not as culture extracts are the following: Chondrostereum purpureum, Irpex lacteus, and Xylobolus frustulatus;
(2)
The fungi that were not active as fruitbody extracts, but were active as culture extracts are the following: Peniophora quercina and Stereum hirsutum.
In some sources, the activity of corticioid fungi against P. aeruginosa, based on basidiomata extracts, has been studied; these data are similar to our results. Namely, it was found that ethanolic extract of Hymenochaete rubiginosa had low antibacterial effect against this bacterium with minimum inhibitory concentration (MIC) = 200 mg/mL []; ethanolic and methanolic extracts of Stereum hirsutum inhibited this bacterium with MIC = 100 mg/mL [].
There are experimental data on extracts from cultured mycelia tested against P. aeruginosa [,], and the species from these experiments can be divided into the following four groups:
(1)
Active both in our study and in the study []: Chondrostereum purpureum;
(2)
Active in our study, not or very little active in the studies [,]: Byssomerulius corium, Irpex lacteus, Peniophora cinerea, P. incarnata, P. limitata, and Steccherinum ochraceum;
(3)
Highly active in the study [], not active in our study: Coniophora arida and Peniophora quercina;
(4)
Not active in our study and in the study []: Stereum hirsutum and Xylodon paradoxus.
The activity of corticioid fungi against K. pneumoniae has been studied very poorly earlier. There are data that filtrate from the culture of Stereum hirsutum moderately inhibited this bacterium [].
Our research showed that the percentage of species whose fruiting bodies contain substances that inhibit, although at very high concentrations, the growth of clinically important pathogenic bacteria is high among corticioid fungi. Based on the taxonomic analysis of the species list, the orders Polyporales and Russulales in general, and the genera Amylocorticium, Peniophora, Phanerochaete, and Phlebia in particular, are the perspective taxa for further searches of antibacterial substances. However, the antibacterial activity of fungal species based on methanol-soluble compounds from basidiomata collected in the wild, is not constant and is influenced by a number of factors that have not yet been clearly defined. There are differences in activity ranges and MBCs for individual bacteria between fungal samples collected in different seasons, from different hosts, and at different developmental stages of the basidiomata. In some cases, one sample showed activity, although at a very high concentration, against 2–3 bacterial strains, whereas another sample of the same species showed no activity.

4. Materials and Methods

4.1. Fungal Samples

The study included 84 samples belonging to 58 species of corticioid fungi. The fruiting bodies of the fungi were collected in fresh or partially dried (in dry weather) states from forests in the north-eastern part of Poland, mainly from the Białowieża Primeval Forest, in all seasons of the years 2017–2023. Most of the extracts were accompanied by the reference herbarium specimens of the fungi from which they were obtained. The reference specimens were deposited in Białystok University of Technology Herbarium–BLS (Appendix A, Table A1). Fungal samples collected by E. Yurchenko were identified by the same author. The names of the fungi are according to MycoBank (https://www.mycobank.org; accessed on 18 October 2024), and the order-level classification follows []. It was assumed that depending on the fungal species and the size of the substratum, the fruiting bodies of one sample came from one or more individual mycelia. In the latter case, the mycelia were from one or more substratum units, but the units were spatially close to each other. In the case of species producing abundant hyphal cords or rhizomorphs (Etheirodon fimbriatum and Phanerochaete velutina), these structures were taken for extraction together with hymenium-bearing parts.

4.2. Extract Preparation

After collecting in the field, the fruiting bodies were checked for colonization by other fungi. The fructifications infected by mycophilous fungi, mixed with other corticioid species, or those in a post-mature (destroyed) state were not used for extraction. In the laboratory, the fruiting bodies were separated with a scalpel, avoiding as far as possible the collection of substratum material, i.e., dead wood or bark. For this procedure, fruiting bodies were rehydrated in a moist chamber, if necessary. Thin fruiting bodies were usually detached from the substratum in fragments of 2–5 mm in size. Large fruiting bodies were cut into 1–3 cm pieces. The dry mass of the fungal material prepared in this way ranged from 0.6 g (Amylocorticium cebennense) to 75 g (Noblesia crocea) per sample.
The collected fungal material was dried at room temperature, weighed, and immersed in methanol (99.8%; Poch Basic—Avantor Performance Materials, Gliwice, Poland) in a ratio of 1:3 (v/v) for larger fungal samples and up to 1:10 (v/v) for smaller samples, in such a way that the fungal material was completely covered by the solvent. Methanol was chosen as the most effective solvent for obtaining extracts with antibacterial potential []. Passive extraction without stirring or shaking took 2 months in the dark at room temperature. In case when the amount of crude extract was too small, a second maceration of the same sample in methanol was carried out for 2 weeks. If the second tincture was dark pigmented, it was evaporated and added to the primary extract.
The finished tinctures were filtered through the 80 g/m2 filter paper and the solvent was evaporated in two steps. In the first step, most of the solvent was removed in a rotary evaporator Rotavapor® R-100 (Büchi, Flawil, Switzerland) at 46 °C, rotation speed 3–4 units, and under the reduced pressure from 300 to 100 mbar. In the second step, the extract was collected from the walls of the extraction round-bottomed flask together with a small amount of methanol and placed in the glass extraction cells of a system of parallel evaporation MultivaporTM P-12 (Büchi), where incubated at 46 °C and under the reduced pressure from 400 to 100 mbar. Each solid-state extract was stored in the dark at 10 °C in the Fungi Extract Bank® collection (https://fungiextractbank.com/en, accessed on 17 February 2025; Appendix A, Table A1).

4.3. Bacterial Strains and Testing the Antibacterial Activity

Strains of bacteria Corynebacterium striatum PCM 3067, Klebsiella pneumoniae PCM 2713, Pseudomonas aeruginosa PCM 2270, and Staphylococcus aureus PCM 2267 were obtained from the Polish Collection of Microorganisms (Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland). The strain Haemophilus influenzae (b) ATCC® 10211 originated from the American Type Culture Collection (Rockville, MD, USA). The bacterial cultures in Mueller-Hinton broth (Merck, Darmstadt, Germany), 24 h post inoculation, with the density of suspension about 1 × 107 colony-forming units/mL, were used as subsequent inocula in the experiments. The bacterial density was determined using the plate method, and inocula were diluted by physiological solution if needed. Liquid inoculum and test cultures of H. influenzae were maintained in tryptic soy broth with hemin and NAD, in microaerophilic conditions (6% CO2). All incubations of bacteria in liquid culture or on agar plates were carried out for 24 h at 37 ± 1 °C.
The antibacterial activity of the fungal extracts was preliminary assessed in glass tubes. Prior to this, the dried extracts were dissolved in dimethyl sulfoxide (DMSO; Merck) at a concentration of 100 mg/mL. DMSO was selected as a good amphiphilic solvent [], which is not very toxic to bacteria in dilute concentrations [,]. These dissolved extracts were then diluted with distilled water to obtain the two test concentrations, 25 mg/mL and 50 mg/mL [], and stored at 4 °C in dark glass bottles before the inoculation with bacteria. Each test tube contained 1.5 mL of broth, 0.5 mL of the tested extract 100 mg/mL, and 0.1 mL of broth inoculum (for the test concentration 25 mg/mL) or 1 mL of broth, 1 mL of the tested extract, and 0.1 mL of broth inoculum (for the test concentration 50 mg/mL). Solvent controls consisted of 1 mL of broth, 1 mL DMSO, and 0.1 mL of inoculum. Reference samples were the broth inoculated with bacteria. Tubes were visually inspected and those with no obvious bacterial growth, i.e., where the medium was not turbid, were considered to have the minimum inhibitory concentration (MIC) of the extract if this concentration followed the lower concentration at which growth was visible.
To check for growth inhibition, the second set of experiments was performed as a microdilution assay in wells of plastic 96-well microplates (Corning®, Corning, NY, USA), but in this case, the combinations of each strain with each extract in two concentrations (25 and 50 mg/mL) were repeated in four wells. Each well contained 100 μL of broth inoculum and 100 μL of dissolved extract. Negative controls contained 100 μL of broth inoculum and 100 μL of distilled water. Solvent controls contained 100 μL of broth inoculum and 100 μL DMSO. Controls were repeated in tetraplicate.
In the third set of experiments, the minimum bactericidal concentration (MBC) was determined from cultures on agar plates. The microplate wells in which no growth of a given microorganism was observed, i.e., with transparent contents, were selected, and a loopful of liquid was taken from them and streaked in a zig-zag pattern on agar plates in 90 mm Petri dish divided into 8 sectors. The media used were Mueller-Hinton agar (Merck) for C. striatum, K. pneumoniae, P. aeruginosa, S. aureus, and chocolate agar with polyvitamin supplement and bacitracin (Bio-Rad, Hercules, CA, USA) for H. influenzae. The MBC value was the concentration of the extract at which no bacterial growth was observed on agar.

Author Contributions

Conceptualization, E.Y.; data curation, E.Y.; formal analysis, E.Y.; funding acquisition, E.Y. and E.Z.; investigation, M.K. and M.K.-S.; methodology, E.Y., M.K., M.K-S. and E.Z.; project administration, E.Y. and E.Z.; resources, E.Y.; writing—original draft, E.Y.; writing—review and editing, E.Z. All authors have read and agreed to the published version of the manuscript.

Funding

This research was supported by a grant from the National Centre for Research and Development (NCBR, Poland) ‘Assessment of the potential of corticoid fungi as a source of substances with activity against bacteria associated with respiratory diseases and causing co-infections, e.g., in COVID-19’ (SzN/2/139/Cortic23/2022). Publication of this article was partly supported by the Białystok University of Technology (work No. WZ/WB-INL/2/2025).

Institutional Review Board Statement

Not applicable.

Data Availability Statement

Data is contained within the article.

Acknowledgments

The authors are grateful to Marek Wołkowycki and Konrad Wilamowski (Institute of Forest Sciences, Białystok University of Technology—IFS, BUT) for their help in collecting the fungi in the wild and to Malgorzata Kowalska (IFS, BUT) for the preparation of tinctures and solid extracts. We acknowledge the contribution to this work of Grzegorz Kuryło, who collected some samples, and of Anna Wołkowycka-Drużba, who prepared some extracts (both formerly associated with IFS, BUT).

Conflicts of Interest

The authors declare no conflicts of interest.

Appendix A

Table A1. Sample and extract data for the research.
Table A1. Sample and extract data for the research.
Fungal SpeciesNo. of Extract in the Fungi Extract Bank®Collector(s) *, Month and Year of CollectingReference Herbarium Specimen No./Field NumberArea, Host
Agaricales
Chondrostereum purpureum238KW, XI.2022 Hajnówka vicinities, Pyrus domestica
Ch. purpureum248EY, KW, XII.2022BLS M-3975/EYu 221229-1Hajnówka vicinities, Cerasus vulgaris
Radulomyces molaris239EY, V.2023BLS M-10053/EYu 230509-6BPF **, Salix caprea
Amylocorticiales
Amylocorticium cebennense344EY, IX.2023BLS M-10832/EYu 230926-8BPF, Picea abies
Amylocorticium subincarnatum336EY, IX.2023BLS M-10840/EYu 230926-1aBPF, Picea abies
Irpicodon pendulus346EY, MW, XI.2023BLS M-10831/EYu 231103-3BPF, Pinus sylvestris
Cantharellales
Botryobasidium subcoronatum303EY, V.2023BLS M-10821/EYu 230525-3BPF, Pinus sylvestris
Coniophorales
Coniophora arida345EY, XI.2023BLS M-10830/EYu 231103-2BPF, Picea abies
Gloeophyllales
Boreostereum radiatum236MW, X.2022BLS M-3982Białystok vicinities, Picea abies
Hymenochaetales
Hydnoporia tabacina78EY, KW, XII.2022BLS M-3974/EYu 221229-5Hajnówka vicinities, Corylus avellana
H. tabacina243MW, X.2018BLS M-603BPF, Salix cinerea
H. tabacina286EY, VI.2023BLS M-10046/EYu 230608-3BPF, Picea abies
Hymenochaete rubiginosa56MW, XI.2021 BPF, Quercus robur
Kneiffiella barba-jovis283EY, VI.2023BLS M-10064/EYu 230608-2BPF, Betula sp.
Lyomyces crustosus292-1EY, VI.2023BLS M-10311/EYu 230625-4BPF, Corylus avellana
L. crustosus292-2EY, VIII.2023BLS M-10323/EYu 230803-1BPF, Corylus avellana
Peniophorella praetermissa349EY, IX.2023BLS M-10842/EYu 230921-4BPF, Fraxinus excelsior
Resinicium bicolor276EY, V.2023BLS M-10054/EYu 230501-1BPF, Picea abies
R. bicolor308EY, IX.2023BLS M-11611/EYu 230907-2BPF, Pinus sylvestris
Skvortzovia furfuracea285EY, V.2023BLS M-10051/EYu 230509-4BPF, Pinus sylvestris
Xylodon brevisetus309EY, IX.2023BLS M-11612/EYu 230907-7BPF, Picea abies
Xylodon nesporii304EY, VII.2023BLS M-10321/EYu 230710-5BPF, Corylus avellana
Xylodon paradoxus265EY, V.2023BLS M-10050/EYu 230524-4BPF, Carpinus betulus
X. paradoxus284EY, V.2023BLS M-10048/EYu 230501-4BPF, Quercus robur
Xylodon spathulatus347EY, MW, IX.2023BLS M-10847/EYu 230919-6Łuków vicinities, Abies alba
Polyporales
Byssomerulius corium245EY, X.2022BLS M-3939/EYu 221009-9BPF, Populus tremula
B. corium264EY, IV.2023BLS M-10063/EYu 230423-1BPF, Carpinus betulus
Crustoderma dryinum271EY, V.2023BLS M-10056/EYu 230511-1BPF, Picea abies
Dacryobolus karstenii348EY, MW, XI.2023BLS M-10829/EYu 231103-1BPF, Pinus sylvestris
Etheirodon fimbriatum333EY, IX.2023BLS M-10838/EYu 230926-2BPF, Alnus glutinosa
E. fimbriatum341EY, X.2023BLS M-10824/EYu 231008-4BPF, Salix caprea
Hyphoderma transiens343EY X.2023BLS M-10826/EYu 231008-6BPF, Tilia cordata
Hyphoderma setigerum222-1EY, X.2022BLS M-3944/EYu 221016-1BPF, Betula pendula
H. setigerum222-2EY, III.2022BLS M-3931/EYu 220320-1BPF, Betula pendula
Irpex lacteus311EY, IX.2023BLS M-10856/EYu 230907-6BPF, Sorbus aucuparia
Meruliopsis taxicola273EY, MW, V.2023BLS M-10060/EYu 230525-2BPF, Pinus sylvestris
Merulius tremellosus170MW, XI.2019 BPF, Populus tremula
Mutatoderma mutatum244EY, X.2022BLS M-3943/EYu 221009-8BPF, Populus tremula
Mycoacia livida335EY, IX.2023BLS M-10843/EYu 230921-3BPF, Fraxinus excelsior
Noblesia crocea (without substratum)302-1MW, VIII.2023BLS M-10309/230815-1aHajnówka vicinities, Malus domestica
N. crocea (the part of basidiomata interspersed with substratum particles)302-2MW, VIII.2023BLS M-10309/230815-1bthe same as above
Phanerochaete sordida291EY, VI.2023BLS M-10314/EYu 230625-5BPF, Fraxinus excelsior
Ph. sordida305EY, VII.2023BLS M-10322/EYu 230710-4BPF, Populus tremula
Phanerochaete velutina334EY, IX.2023BLS M-10844/EYu 230921-2BPF, Fraxinus excelsior
Phlebia centrifuga202-1GK, VIII.2020 BPF, Picea abies
Ph. centrifuga202-2EY, X.2023BLS M-3956/EYu 221016-7BPF, Picea abies
Phlebia rufa229EY, VIII.2022BLS M-3565/EYu 220802-12BPF, Corylus avellana
Ph. rufa232EY, V.2022BLS M-11610/EYu 230501-52 BPF, Fraxinus excelsior
Phlebiodontia cf. subochracea 313EY, MW, IX.2023BLS M-10846/EYu 230919-7Łuków vicinities, Abies alba
Phlebiopsis gigantea247-1EY, KW, XII.2022BLS M-3977/EYu 221229-4Hajnówka vicinities, Pinus sylvestris
Ph. gigantea247-2EY, V.2023BLS M-10059/EYu 230501-8BPF, Pinus sylvestris
Scopuloides hydnoides338EY, IX.2023BLS M-10835/EYu 230926-5BPF, Fraxinus excelsior
Steccherinum bourdotii339EY, MW, IX.2023BLS M-10850/EYu 230919-1Łuków vicinities, Acer pseudoplatanus
Steccherinum ochraceum296EY, VII.2023BLS M-10315/EYu 230702-2aBPF, Carpinus betulus
S. ochraceum340EY, IX.2023BLS M-10837/EYu 230926-3BPF, Alnus glutinosa
Russulales
Asterostroma medium312EY, IX.2023BLS M-10845/EYu 230919-8Łuków vicinities, Abies alba
Baltazaria galactina231EY, VIII.2022BLS M-3563/EYu 220802-4BPF, Populus tremula
B. galactina233MW, IX.2022BLS M-3981BPF, Tilia cordata
Dentipellis fragilis342MW, X.2023BLS M-10828BPF, Alnus glutinosa
Gloiothele lactescens314EY, IX.2023BLS M-10841/EYu230921-5BPF, cf. Fraxinus excelsior
Laxitextum bicolor178MW, IX.2020BLS M-3630BPF, Salix caprea
L. bicolor235MW, X.2022BLS M-3996Białystok vicinities, Salix caprea
Peniophora cinerea293EY, VI.2023BLS M-10325/EYu 230625-2BPF, Alnus glutinosa
P. cinerea295EY, VII.2023BLS M-10316/EYu 230710-3BPF, Corylus avellana
P. cinerea310EY, IX.2023BLS M-10857/EYu 230907-4BPF, Sorbus aucuparia
Peniophora incarnata272EY, V.2023BLS M-10062/EYu 230501-5BPF, Populus tremula
Peniophora laeta288-1EY, V.2023BLS M-10052/EYu 230509-7BPF, Carpinus betulus
P. laeta288-2EY, VIII.2023BLS M-10324/EYu 230803-2BPF, Carpinus betulus
Peniophora limitata290EY, V.2023BLS M-10327/EYu 230509-8BPF, Fraxinus excelsior
Peniophora pithya287EY, V.2023BLS M-10047/EYu 230509-2BPF, Picea abies
Peniophora quercina237EY, X.2022BLS M-3957/EYu 221016-9BPF, Quercus robur
Peniophora rufomarginata249-1MW, XII.2022BLS M-10070BPF, Tilia cordata
P. rufomarginata249-2EY, X.2022BLS M-3938/EYu 221009-10BPF, Tilia cordata
Scytinostroma odoratum337EY, MW, IX.2023BLS M-10849/EYu 230919-3Łuków vicinities, Pinus sylvestris
Stereum hirsutum167MW, XII.2020 BPF, Carpinus betulus
S. hirsutum289EY, VI.2023BLS M-10317/EYu 230625-2BPF, Alnus glutinosa
S. hirsutum294-1EY, VII.2023BLS M-10308/EYu 230710-1BPF, Quercus robur
S. hirsutum294-2EY, VIII.2023BLS M-10320/EYu 230803-3BPF, Quercus robur
Stereum rugosum79EY, IV.2023BLS M-10061/EYu 230423-4BPF, Corylus avellana
S. rugosum281EY, V.2023BLS M-10065/EYu 230501-3BPF, Quercus robur
Stereum sanguinolentum274EY, V.2023BLS M-10055/EYu 230509-1BPF, Picea abies
S. sanguinolentum275EY, V.2023BLS M-10058/EYu 230511-6BPF, Pinus sylvestris
Stereum subtomentosum197MW, X.2019BLS M-1946BPF, Alnus glutinosa
Xylobolus frustulatus186-1GK, VI.2021 BPF, Quercus robur
X. frustulatus186-2EY, IV.2023BLS M-10069/EYu 230423-3BPF, Quercus robur
* EY—Eugene Yurchenko, GK—Grzegorz Kuryło, KW—Konrad Wilamowski, MW—Marek Wołkowycki. ** BPF—Białowieża Primeval Forest, the area between Hajnówka and Białowieża, including Ladzka Primeval Forest.

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