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Communication

Establishment of a Panel of Human Cell Lines to Identify Cellular Receptors Used by Enteroviruses to Infect Cells

by
Anastasiia O. Sosnovtseva
1,2,*,
Thi Hoa Le
3,
Dmitry S. Karpov
1,2,
Pavel O. Vorobyev
1,2,
Yana D. Gumennaya
2,
Olga N. Alekseeva
2,
Peter M. Chumakov
1,2 and
Anastasia V. Lipatova
1,2
1
Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia
2
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia
3
Department of Molecular Microbiology and Immunology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(3), 923; https://doi.org/10.3390/ijms26030923
Submission received: 26 November 2024 / Revised: 20 January 2025 / Accepted: 21 January 2025 / Published: 22 January 2025
(This article belongs to the Special Issue The Evolution, Genetics and Pathogenesis of Viruses)
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Abstract

Non-pathogenic natural and recombinant strains of human Enteroviruses are the subject of ongoing study with some strains having been approved for use as anticancer agents. The efficacy of oncolytic virotherapy depends upon identifying the receptor utilized by a specific strain for cell entry, and the presence of this receptor on the surface of cancer cells. Accordingly, a rapid and straightforward approach to determining the enteroviral receptors is necessary for developing an effective patient-specific, virus-based cancer therapy. To this end, we created a panel of seven lines with double knockouts on the background of the HEK293T cell line, which lacks the IFNAR1 gene. In these lines, the main viral receptor genes, including PVR, CXADR, CD55, ITGA2, SCARB2, ICAM1, and FCGRT, were knocked out using the CRISPR/Cas9 system. The panel of lines was validated on twelve different Enteroviruses types, providing a basis for studying the molecular mechanisms of enterovirus entry into cells, and for developing new therapeutic strains.
Keywords: CRISPR/Cas9; Enteroviruses; viral phylogeny; coxsackievirus; echovirus CRISPR/Cas9; Enteroviruses; viral phylogeny; coxsackievirus; echovirus

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MDPI and ACS Style

Sosnovtseva, A.O.; Le, T.H.; Karpov, D.S.; Vorobyev, P.O.; Gumennaya, Y.D.; Alekseeva, O.N.; Chumakov, P.M.; Lipatova, A.V. Establishment of a Panel of Human Cell Lines to Identify Cellular Receptors Used by Enteroviruses to Infect Cells. Int. J. Mol. Sci. 2025, 26, 923. https://doi.org/10.3390/ijms26030923

AMA Style

Sosnovtseva AO, Le TH, Karpov DS, Vorobyev PO, Gumennaya YD, Alekseeva ON, Chumakov PM, Lipatova AV. Establishment of a Panel of Human Cell Lines to Identify Cellular Receptors Used by Enteroviruses to Infect Cells. International Journal of Molecular Sciences. 2025; 26(3):923. https://doi.org/10.3390/ijms26030923

Chicago/Turabian Style

Sosnovtseva, Anastasiia O., Thi Hoa Le, Dmitry S. Karpov, Pavel O. Vorobyev, Yana D. Gumennaya, Olga N. Alekseeva, Peter M. Chumakov, and Anastasia V. Lipatova. 2025. "Establishment of a Panel of Human Cell Lines to Identify Cellular Receptors Used by Enteroviruses to Infect Cells" International Journal of Molecular Sciences 26, no. 3: 923. https://doi.org/10.3390/ijms26030923

APA Style

Sosnovtseva, A. O., Le, T. H., Karpov, D. S., Vorobyev, P. O., Gumennaya, Y. D., Alekseeva, O. N., Chumakov, P. M., & Lipatova, A. V. (2025). Establishment of a Panel of Human Cell Lines to Identify Cellular Receptors Used by Enteroviruses to Infect Cells. International Journal of Molecular Sciences, 26(3), 923. https://doi.org/10.3390/ijms26030923

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