Next Article in Journal
Coenzyme Q10 Ameliorates Chemotherapy-Induced Neurotoxicity in iPSC-Derived Neurons by Reducing Oxidative Stress
Previous Article in Journal
Connections Between Gene Polymorphism and Fetlock and Hock Measurements in Polish Sport Horses
Previous Article in Special Issue
Differentiation of Human Pluripotent Cells into Pancreatic Beta Cells for Disease Modeling and Cell Replacement Therapy for Diabetes
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 26
Issue 19
10.3390/ijms26199646
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

T-Cadherin Finetunes Proliferation–Differentiation During Adipogenesis via PI3K–AKT Signaling Pathway

by
Polina Klimovich
1,2,
Ilya Brodsky
1,
Valentina Dzreyan
1,
Marianna Ivleva
1,
Olga Grigorieva
3,
Mark Meshcheriakov
1,
Ekaterina Semina
1,
Veronika Sysoeva
1,
Vsevolod Tkachuk
1,2 and
Kseniya Rubina
1,*
1
Faculty of Medicine, Lomonosov Moscow State University, 119991 Moscow, Russia
2
Institute of Experimental Cardiology, National Medical Research Center of Cardiology Named after Academician E.I. Chazov, 121552 Moscow, Russia
3
Center for Regenerative Medicine, Medical Research and Education Institute, Lomonosov Moscow State University, 119192 Moscow, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(19), 9646; https://doi.org/10.3390/ijms26199646
Submission received: 27 August 2025 / Revised: 23 September 2025 / Accepted: 30 September 2025 / Published: 2 October 2025
(This article belongs to the Special Issue Fundamental and Practical Perspectives in Regenerative Medicine: Proceedings of the VI National Congress of Regenerative Medicine (2024))
Review Reports Versions Notes

Abstract

Adipose tissue renewal requires precise coordination of stem/progenitor cell proliferation, preadipocyte commitment, and terminal adipocyte differentiation. T-cadherin (CDH13), an atypical GPI-anchored cadherin, is expressed in adipose tissue and functions as a receptor for high-molecular-weight (HMW) adiponectin—a key adipokine produced by adipose tissue and involved in metabolic regulation. While T-cadherin is implicated in cardiovascular and metabolic homeostasis, its role in adipogenesis still remains poorly understood. In this study, we used the 3T3-L1 preadipocyte model to investigate the function of T-cadherin in adipocyte differentiation. We analyzed T-cadherin expression dynamics during differentiation and assessed how T-cadherin overexpression or knockdown affects lipid accumulation, expression of adipogenic markers, and key signaling pathways including ERK, PI3K–AKT, AMPK, and mTOR. Our findings demonstrate that T-cadherin acts as a negative regulator of adipogenesis. T-cadherin overexpression ensured a proliferative, undifferentiated cell state, delaying early adipogenic differentiation and suppressing both lipid droplet accumulation and the expression of adipogenic markers. In contrast, T-cadherin downregulation accelerated differentiation, enhanced lipid accumulation, and increased insulin responsiveness, as indicated by PI3K–AKT pathway activation at specific stages of adipogenesis. These results position T-cadherin as a key modulator of adipose tissue plasticity, regulating the balance between progenitor expansion and terminal differentiation, with potential relevance to obesity and metabolic disease.
Keywords: T-cadherin; CDH13; adiponectin; adipogenesis; 3T3-L1; insulin signaling T-cadherin; CDH13; adiponectin; adipogenesis; 3T3-L1; insulin signaling

Share and Cite

MDPI and ACS Style

Klimovich, P.; Brodsky, I.; Dzreyan, V.; Ivleva, M.; Grigorieva, O.; Meshcheriakov, M.; Semina, E.; Sysoeva, V.; Tkachuk, V.; Rubina, K. T-Cadherin Finetunes Proliferation–Differentiation During Adipogenesis via PI3K–AKT Signaling Pathway. Int. J. Mol. Sci. 2025, 26, 9646. https://doi.org/10.3390/ijms26199646

AMA Style

Klimovich P, Brodsky I, Dzreyan V, Ivleva M, Grigorieva O, Meshcheriakov M, Semina E, Sysoeva V, Tkachuk V, Rubina K. T-Cadherin Finetunes Proliferation–Differentiation During Adipogenesis via PI3K–AKT Signaling Pathway. International Journal of Molecular Sciences. 2025; 26(19):9646. https://doi.org/10.3390/ijms26199646

Chicago/Turabian Style

Klimovich, Polina, Ilya Brodsky, Valentina Dzreyan, Marianna Ivleva, Olga Grigorieva, Mark Meshcheriakov, Ekaterina Semina, Veronika Sysoeva, Vsevolod Tkachuk, and Kseniya Rubina. 2025. "T-Cadherin Finetunes Proliferation–Differentiation During Adipogenesis via PI3K–AKT Signaling Pathway" International Journal of Molecular Sciences 26, no. 19: 9646. https://doi.org/10.3390/ijms26199646

APA Style

Klimovich, P., Brodsky, I., Dzreyan, V., Ivleva, M., Grigorieva, O., Meshcheriakov, M., Semina, E., Sysoeva, V., Tkachuk, V., & Rubina, K. (2025). T-Cadherin Finetunes Proliferation–Differentiation During Adipogenesis via PI3K–AKT Signaling Pathway. International Journal of Molecular Sciences, 26(19), 9646. https://doi.org/10.3390/ijms26199646

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop