Transcriptional Regulation of WTAP Isoforms by NF-κB Signaling in Human Monocytes
Abstract
1. Introduction
2. Results
2.1. Alternative Promoter Utilization of WTAP in Human CD14+ Monocyte Activation
2.2. Transcription Factor RelA Promotes Transcriptional Initiation of Specific WTAP Isoforms in Monocyte Activation
3. Discussion
4. Materials and Methods
4.1. Cell Culturing
4.2. qPCR
4.3. Western Blot
4.4. Nanopore Sequencing
4.5. Next Generation Sequencing
4.6. Transfection of HEK293T Cells with WTAP Isoforms
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Picavet, L.W.; Abu-Tawil, H.I.; Sijbers, L.J.P.M.; Calis, J.J.A.; ter Haar, N.; Bodelón, A.; Vastert, S.J.; van Loosdregt, J. Transcriptional Regulation of WTAP Isoforms by NF-κB Signaling in Human Monocytes. Int. J. Mol. Sci. 2025, 26, 9364. https://doi.org/10.3390/ijms26199364
Picavet LW, Abu-Tawil HI, Sijbers LJPM, Calis JJA, ter Haar N, Bodelón A, Vastert SJ, van Loosdregt J. Transcriptional Regulation of WTAP Isoforms by NF-κB Signaling in Human Monocytes. International Journal of Molecular Sciences. 2025; 26(19):9364. https://doi.org/10.3390/ijms26199364
Chicago/Turabian StylePicavet, Lucas W., Hisham I. Abu-Tawil, Lyanne J. P. M. Sijbers, Jorg J. A. Calis, Nienke ter Haar, Alejandra Bodelón, Sebastiaan J. Vastert, and Jorg van Loosdregt. 2025. "Transcriptional Regulation of WTAP Isoforms by NF-κB Signaling in Human Monocytes" International Journal of Molecular Sciences 26, no. 19: 9364. https://doi.org/10.3390/ijms26199364
APA StylePicavet, L. W., Abu-Tawil, H. I., Sijbers, L. J. P. M., Calis, J. J. A., ter Haar, N., Bodelón, A., Vastert, S. J., & van Loosdregt, J. (2025). Transcriptional Regulation of WTAP Isoforms by NF-κB Signaling in Human Monocytes. International Journal of Molecular Sciences, 26(19), 9364. https://doi.org/10.3390/ijms26199364