Next Article in Journal
Molecular Mechanisms of Herbicide Resistance in Rapeseed: Current Status and Future Prospects for Resistant Germplasm Development
Previous Article in Journal
Papillary Thyroid Carcinoma and Body Mass Index: The Role of Immune System in Tumor Microenvironment
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 26
Issue 17
10.3390/ijms26178288
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

U11/U12 Small Nuclear Ribonucleoprotein TaU11/U12-35K Interacts with TaHis and Negatively Contributes to Fusarium Head Blight Resistance in Wheat

by
Puwen Song
1,2,3,†,
Ao Li
1,†,
Jiale Deng
1,
Dan Li
1,
Ping Hu
1,
Yuanyuan Guan
1,
Meng Zhang
1,
Qili Liu
1,
Haiyan Hu
1,* and
Zhengang Ru
1,3,*
1
School of Agriculture, State Key Laboratory of Wheat-Maize Double Cropping and High-Efficiency Production, Henan Engineering Research Center of Crop Genome Editing, Henan Institute of Science and Technology, Xinxiang 453003, China
2
Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, Henan Institute of Science and Technology, Xinxiang 453003, China
3
Center of Wheat Research, Henan Institute of Science and Technology, Xinxiang 450003, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2025, 26(17), 8288; https://doi.org/10.3390/ijms26178288 (registering DOI)
Submission received: 30 July 2025 / Revised: 20 August 2025 / Accepted: 23 August 2025 / Published: 26 August 2025
(This article belongs to the Section Molecular Plant Sciences)
Versions Notes

Abstract

Fusarium head blight (FHB), caused by Fusarium graminearum (F. graminearum), has become one of the most devastating wheat diseases, severely impacting both grain yield and quality. The resistance gene TaHis (encoding a histidine-rich calcium-binding protein), located at the major FHB resistance locus Fhb1, has been demonstrated to confer FHB resistance in wheat, although its underlying mechanism remains unclear. In this study, we screened a wheat yeast two-hybrid (Y2H) library and identified TaU11/U12-35K, a core component of the U12-type spliceosome (U11/U12 small nuclear ribonucleoprotein), as a novel interacting partner of TaHis. Their physical interaction was further confirmed by both Y2H and bimolecular fluorescence complementation assays. Barley stripe mosaic virus-induced gene silencing (BSMV-VIGS)-mediated knockdown of TaU11/U12-35K significantly enhanced FHB resistance in both resistant (Bainong 4299) and susceptible (Bainong 5819) cultivars compared to controls. Expression profiling revealed that TaU11/U12-35K was significantly downregulated upon F. graminearum infection in both cultivars, with consistently lower basal expression levels in Bainong 4299, suggesting a negative correlation between TaU11/U12-35K expression and FHB resistance. Collectively, our results demonstrate that TaU11/U12-35K physically interacts with TaHis and functions as a negative regulator of FHB resistance. This study provides new insights into the molecular mechanism of TaHis-mediated FHB resistance in wheat.
Keywords: Fusarium head blight; TaHis; TaU11/U12-35K Fusarium head blight; TaHis; TaU11/U12-35K

Share and Cite

MDPI and ACS Style

Song, P.; Li, A.; Deng, J.; Li, D.; Hu, P.; Guan, Y.; Zhang, M.; Liu, Q.; Hu, H.; Ru, Z. U11/U12 Small Nuclear Ribonucleoprotein TaU11/U12-35K Interacts with TaHis and Negatively Contributes to Fusarium Head Blight Resistance in Wheat. Int. J. Mol. Sci. 2025, 26, 8288. https://doi.org/10.3390/ijms26178288

AMA Style

Song P, Li A, Deng J, Li D, Hu P, Guan Y, Zhang M, Liu Q, Hu H, Ru Z. U11/U12 Small Nuclear Ribonucleoprotein TaU11/U12-35K Interacts with TaHis and Negatively Contributes to Fusarium Head Blight Resistance in Wheat. International Journal of Molecular Sciences. 2025; 26(17):8288. https://doi.org/10.3390/ijms26178288

Chicago/Turabian Style

Song, Puwen, Ao Li, Jiale Deng, Dan Li, Ping Hu, Yuanyuan Guan, Meng Zhang, Qili Liu, Haiyan Hu, and Zhengang Ru. 2025. "U11/U12 Small Nuclear Ribonucleoprotein TaU11/U12-35K Interacts with TaHis and Negatively Contributes to Fusarium Head Blight Resistance in Wheat" International Journal of Molecular Sciences 26, no. 17: 8288. https://doi.org/10.3390/ijms26178288

APA Style

Song, P., Li, A., Deng, J., Li, D., Hu, P., Guan, Y., Zhang, M., Liu, Q., Hu, H., & Ru, Z. (2025). U11/U12 Small Nuclear Ribonucleoprotein TaU11/U12-35K Interacts with TaHis and Negatively Contributes to Fusarium Head Blight Resistance in Wheat. International Journal of Molecular Sciences, 26(17), 8288. https://doi.org/10.3390/ijms26178288

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop