Asynchrony Between Endometrial miRNA- and mRNA-Based Receptivity Stages Associated with Impaired Receptivity in Recurrent Implantation Failure
Abstract
1. Introduction
2. Results
2.1. Concordance Between mRNA (ERA) and miRNA (MIRA) Profiles
2.2. Staging Synchrony Between mRNA (ERA) and miRNA (MIRA) Profiles
2.3. Clinical Factors Associated with Lagging miRNA-Based Relative to mRNA-Based Receptivity Staging
2.4. Function Enrichment Analysis for Those miRNAs with Differential Expression in the Slow Group
3. Discussion
4. Materials and Methods
4.1. Study Design and Patient Population
4.2. Endometrial Receptivity Array (ERA) Analysis
4.3. MicroRNA Receptivity Assay (MIRA) Analysis
4.4. Group Classification Based on miRNA and mRNA Timing
4.5. Personalized Embryo Transfer (ET) and Pregnancy Outcomes
4.6. Statistical Analysis
4.7. Functional Enrichment Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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ERA (mRNA Profiles) | MIRA (microRNA Profiles) | Total | ||
---|---|---|---|---|
Pre-Receptive (P + 4)mi | Receptive (P + 5)mi | Post-Receptive (P + 6)mi | ||
Pre-receptive (P + 4)m | 13 | 9 | 0 | 22 (22.0%) |
Receptive (P + 5)m | 6 | 51 | 8 | 65 (65.0%) |
Post-receptive (P + 6)m | 1 | 4 | 8 | 13 (13.0%) |
Total | 20 (20.0%) | 64 (64.0%) | 16 (16.0%) | 100 |
Pregnancy Rate | Fast (n = 17) | Equal (n = 72) | Slow (n = 11) | p 1 |
---|---|---|---|---|
ERA pre-receptive | 8/9 (88.9%) | 10/13 (76.9%) | NA | 0.485 |
ERA receptive | 8/8 (100%) | 42/51 (82.4%) | 4/6 (66.7%) | 0.247 |
ERA post-receptive | NA | 7/8 (87.5%) | 2/5 (40.0%) | 0.083 |
Total | 16/17 (94.1%) | 59/72 (81.9%) | 6/11 (54.5%) | 0.031 |
Clinical Factors | Fast (n = 17) | Equal (n = 72) | Slow (n = 11) | p 1 |
---|---|---|---|---|
Age (Y) | 36.0 (34.0–42.0) | 39.0 (36.5–43.0) | 40.0 (35.5–45.5) | 0.260 |
BMI (Kg/m2) | 20.7 (18.8–23.1) | 21.5 (20.3–23.4) | 21.1 (19.3–23.1) | 0.524 |
Duration of infertility (Y) | 2.4 (1.7–4.1) | 3.3 (2.0–5.2) | 4.0 (1.5–6.8) | 0.633 |
AMH (ng/mL) | 1.52 (0.22–3.21) | 2.07 (0.70–3.91) | 2.09 (0.88–7.03) | 0.334 |
Embryo transfer times | 3.0 (2.0–3.3) | 3.0 (2.0–3.0) | 3.0 (2.0–3.8) | 0.875 |
PCOS (%) | 2 (11.8%) | 9 (12.5%) | 4 (36.4%) | 0.109 |
Endometriosis (%) | 5 (29.4%) | 17 (23.6%) | 3 (27.3%) | 0.869 |
Endometrial polyps (%) | 10 (58.8%) | 23 (31.9%) | 2 (18.2%) | 0.019 |
Clinical pregnancy (%) | 16 (94.1%) | 59 (81.9%) | 6 (54.5%) | 0.013 |
Abortion (%) | 3 (17.6%) | 7 (9.7%) | 0 (0%) | 0.128 |
Live birth (%) | 13 (76.5%) | 52 (72.2%) | 6 (54.5%) | 0.251 |
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Lee, Y.-J.; Lee, C.-Y.; Cheng, E.-H.; Chen, W.-M.; Yang, P.E.; Lee, C.-I.; Lee, T.-H.; Lee, M.-S. Asynchrony Between Endometrial miRNA- and mRNA-Based Receptivity Stages Associated with Impaired Receptivity in Recurrent Implantation Failure. Int. J. Mol. Sci. 2025, 26, 7349. https://doi.org/10.3390/ijms26157349
Lee Y-J, Lee C-Y, Cheng E-H, Chen W-M, Yang PE, Lee C-I, Lee T-H, Lee M-S. Asynchrony Between Endometrial miRNA- and mRNA-Based Receptivity Stages Associated with Impaired Receptivity in Recurrent Implantation Failure. International Journal of Molecular Sciences. 2025; 26(15):7349. https://doi.org/10.3390/ijms26157349
Chicago/Turabian StyleLee, Yu-Jen, Chi-Ying Lee, En-Hui Cheng, Wei-Ming Chen, Pok Eric Yang, Chun-I Lee, Tsung-Hsien Lee, and Maw-Sheng Lee. 2025. "Asynchrony Between Endometrial miRNA- and mRNA-Based Receptivity Stages Associated with Impaired Receptivity in Recurrent Implantation Failure" International Journal of Molecular Sciences 26, no. 15: 7349. https://doi.org/10.3390/ijms26157349
APA StyleLee, Y.-J., Lee, C.-Y., Cheng, E.-H., Chen, W.-M., Yang, P. E., Lee, C.-I., Lee, T.-H., & Lee, M.-S. (2025). Asynchrony Between Endometrial miRNA- and mRNA-Based Receptivity Stages Associated with Impaired Receptivity in Recurrent Implantation Failure. International Journal of Molecular Sciences, 26(15), 7349. https://doi.org/10.3390/ijms26157349