Polynucleotide Mixture Attenuates Ultraviolet B-Induced Skin Pigmentation
Abstract
1. Introduction
2. Results
2.1. PNM, PN, and AM Decreased Oxidative Stress and NF-κB in UVB-Irradiated Keratinocytes
2.2. PNM, PN, and AM Decreased NLRP3 Inflammasomes and IL-18 in UVB-Irradiated Keratinocytes
2.3. PNM, PN, and AM Decreased Melanogenesis-Related Signals in the Melanocyte
2.4. PN and PNM Decreased Oxidative Stress and NLRP3 Inflammasome Activation in UVB-Irradiated Skin
2.5. PN and PNM Decreased Melanogenesis Signals and Melanin Accumulation in UVB-Irradiated Skin
3. Discussion
4. Materials and Methods
4.1. AM, PN, and PNM Preparation
4.2. Cell Culture
4.3. Experimental Design for AM, PN, or PNM Treatment In Vitro
4.3.1. Establishment of a UVB Exposure Model for Keratinocytes
4.3.2. Establishment of CM-Treated Model for Melanoma
4.4. Animal Studies
4.4.1. Mouse Model and Maintenance
4.4.2. Experimental Design for PNM or PN Injections in UVB-Induced Pigmentation Animal Model
4.4.3. Skin Brightness Assessment
4.5. Sample Preparation
4.5.1. Protein Isolation and Concentration Quantification
4.5.2. Preparation of Paraffin-Embedded Skin Tissue Blocks
4.6. Cell Viability Assessment
4.7. Measurement of GSH:GSSG Ratio and Tyrosinase Activity
4.8. Western Blot Analysis
4.9. Enzyme-Linked Immunosorbent Assay (ELISA)
4.10. Staining
4.10.1. Immunocytochemistry
4.10.2. Immunohistochemistry
4.10.3. Fontana–Masson Staining
4.11. Melanin Amount
4.12. Synergy Analysis Using the Bliss Independence Model
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Oh, S.; Jeon, H.-D.; Rho, N.-K.; Son, K.H.; Byun, K. Polynucleotide Mixture Attenuates Ultraviolet B-Induced Skin Pigmentation. Int. J. Mol. Sci. 2025, 26, 6399. https://doi.org/10.3390/ijms26136399
Oh S, Jeon H-D, Rho N-K, Son KH, Byun K. Polynucleotide Mixture Attenuates Ultraviolet B-Induced Skin Pigmentation. International Journal of Molecular Sciences. 2025; 26(13):6399. https://doi.org/10.3390/ijms26136399
Chicago/Turabian StyleOh, Seyeon, Hee-Dae Jeon, Nark-Kyoung Rho, Kuk Hui Son, and Kyunghee Byun. 2025. "Polynucleotide Mixture Attenuates Ultraviolet B-Induced Skin Pigmentation" International Journal of Molecular Sciences 26, no. 13: 6399. https://doi.org/10.3390/ijms26136399
APA StyleOh, S., Jeon, H.-D., Rho, N.-K., Son, K. H., & Byun, K. (2025). Polynucleotide Mixture Attenuates Ultraviolet B-Induced Skin Pigmentation. International Journal of Molecular Sciences, 26(13), 6399. https://doi.org/10.3390/ijms26136399