Role of Extracellular Vesicles of Stem Cells from Human Exfoliated Deciduous Teeth in Osteogenesis
Abstract
1. Introduction
2. Results
2.1. Identification of EVs Extracted from SHED
2.2. Cell Proliferation and Migration Ability of SHED-EVs
2.3. Bone Regeneration Potential of SHED-Evs
3. Discussion
4. Materials and Methods
4.1. Isolation and Culture of SHED and HCOs
4.2. Isolation and Purification of EVs Derived from SHED
4.3. Identification of EVs Derived from SHED
4.3.1. Transmission Electron Microscopy
4.3.2. Nanoparticle Tracking Analysis of EVs
4.4. Cell Proliferation Test
4.4.1. Observation of Cell Proliferative Potential Using Live Cell Imaging System
4.4.2. Evaluation of the Cell Proliferative Potential via BrdU
4.4.3. Scratch Wound Assay
4.5. Evaluation of Alkaline Phosphatase Expression
4.5.1. Real-Time Polymerase Chain Reaction
4.5.2. ALP Quantification
4.5.3. Alizarin Red S Staining
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
MSC | mesenchymal stem cells |
DPSC | dental pulp stem cells |
SHEDs | stem cells from human exfoliated deciduous teeth |
BMSCs | bone mesenchymal stem cells |
BMP | bone morphogenetic protein |
ALP | alkaline phosphatase |
ARS | Alizarin Red S |
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Parameters | Merged Data (nm) | Standard Error of the Mean (nm) |
---|---|---|
Mean | 102.2 | 1.4 |
Mode | 79.9 | 3.2 |
SD | 36 | 1.1 |
D10 | 70.6 | 1.4 |
D50 | 93.5 | 1.4 |
D90 | 142.3 | 2.1 |
Gene | Sequence (5′→3′) |
---|---|
GAPDH (Forward) | GGCCTCCAAGGAGTAAGACC |
GAPDH (Reverse) | AGGGGTCTACATGGCAACTG |
ALP (Forward) | AGAATCTGGTGCAGGAATGG |
ALP (Reverse) | CATGAGATGGGTCACAGACG |
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Shibata, R.; Kunimatsu, R.; Ito, S.; Ogasawara, T.; Ogashira, S.; Nakatani, A.; Rikitake, K.; Odo, A.; Hirabae, A.; Koyanagi, I.; et al. Role of Extracellular Vesicles of Stem Cells from Human Exfoliated Deciduous Teeth in Osteogenesis. Int. J. Mol. Sci. 2025, 26, 5841. https://doi.org/10.3390/ijms26125841
Shibata R, Kunimatsu R, Ito S, Ogasawara T, Ogashira S, Nakatani A, Rikitake K, Odo A, Hirabae A, Koyanagi I, et al. Role of Extracellular Vesicles of Stem Cells from Human Exfoliated Deciduous Teeth in Osteogenesis. International Journal of Molecular Sciences. 2025; 26(12):5841. https://doi.org/10.3390/ijms26125841
Chicago/Turabian StyleShibata, Rio, Ryo Kunimatsu, Shota Ito, Tomohiro Ogasawara, Shintaro Ogashira, Ayaka Nakatani, Kodai Rikitake, Ayaka Odo, Akira Hirabae, Io Koyanagi, and et al. 2025. "Role of Extracellular Vesicles of Stem Cells from Human Exfoliated Deciduous Teeth in Osteogenesis" International Journal of Molecular Sciences 26, no. 12: 5841. https://doi.org/10.3390/ijms26125841
APA StyleShibata, R., Kunimatsu, R., Ito, S., Ogasawara, T., Ogashira, S., Nakatani, A., Rikitake, K., Odo, A., Hirabae, A., Koyanagi, I., Abe, T., Hiraki, T., Sakata, S., Yoshimi, Y., & Tanimoto, K. (2025). Role of Extracellular Vesicles of Stem Cells from Human Exfoliated Deciduous Teeth in Osteogenesis. International Journal of Molecular Sciences, 26(12), 5841. https://doi.org/10.3390/ijms26125841