Next Article in Journal
Prostaglandins Differentially Regulate the Constitutive and Mechanosensitive Release of Soluble Nucleotidases in the Urinary Bladder Mucosa
Next Article in Special Issue
Identification of HXK Gene Family and Expression Analysis of Salt Tolerance in Buchloe dactyloides
Previous Article in Journal
Porter 6: Protein Secondary Structure Prediction by Leveraging Pre-Trained Language Models (PLMs)
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 26
Issue 1
10.3390/ijms26010132
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Cytological, Physiological, and Transcriptome Analysis of Leaf-Yellowing Mutant in Camellia chekiangoleosa

by
Bin Huang
1,
Wenyin Huang
1,
Zhenyu Liu
2,
Yixuan Peng
1,
Yanshu Qu
1,
Wencai Zhou
1,
Jianjian Huang
1,
Huili Shu
1 and
Qiang Wen
1,*
1
Jiangxi Provincial Key Laboratory of Oil-Tea Camellia Resource Cultivation and Utilization, Jiangxi Academy of Forestry, Nanchang 330032, China
2
College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(1), 132; https://doi.org/10.3390/ijms26010132
Submission received: 4 December 2024 / Revised: 23 December 2024 / Accepted: 24 December 2024 / Published: 27 December 2024
(This article belongs to the Special Issue Molecular Research in Bamboo, Tree, Grass, and Other Forest Products)
Browse Figures
Versions Notes

Abstract

Color variation in plant leaves has a significant impact on their photosynthesis and plant growth. Camellia chekiangoleosa yellow-leaf mutants are ideal materials for studying the mechanisms of pigment synthesis and photosynthesis, but their mechanism of leaf variation is not clear. We systematically elucidated the intrinsic causes of leaf yellowing in the new Camellia chekiangoleosa variety ‘Diecui Liuji’ in terms of changes in its cell structure, pigment content, and transcript levels. This study indicates that the incomplete structure of chloroplast-like vesicles, the decrease in blue-green chlorophyll a, and the increase in yellow-green chlorophyll b in yellowing leaves are the direct causes of yellowing-leaf formation. The high expression of genes that catalyze the degradation of chlorophyll a (PAO and RCCR) and its conversion to chlorophyll b (CAO) in yellowing leaves leads to a decrease in the chlorophyll a content, while the low expression of CLH genes is the main reason for the increase in the chlorophyll b content. We also found transcription factors such as ERF, E2F, WRKY, MYB, TPC, TGA, and NFYC may regulate their expression. RT-qPCR assays of 12 DEGs confirm the RNA-seq results. This study will provide a foundation for investigating the transcriptional and regulatory mechanisms of leaf color changes.
Keywords: Camellia chekiangoleosa; leaf-yellowing mutant; physiological; cytological; transcriptome; co-expression regulatory network Camellia chekiangoleosa; leaf-yellowing mutant; physiological; cytological; transcriptome; co-expression regulatory network

Share and Cite

MDPI and ACS Style

Huang, B.; Huang, W.; Liu, Z.; Peng, Y.; Qu, Y.; Zhou, W.; Huang, J.; Shu, H.; Wen, Q. Cytological, Physiological, and Transcriptome Analysis of Leaf-Yellowing Mutant in Camellia chekiangoleosa. Int. J. Mol. Sci. 2025, 26, 132. https://doi.org/10.3390/ijms26010132

AMA Style

Huang B, Huang W, Liu Z, Peng Y, Qu Y, Zhou W, Huang J, Shu H, Wen Q. Cytological, Physiological, and Transcriptome Analysis of Leaf-Yellowing Mutant in Camellia chekiangoleosa. International Journal of Molecular Sciences. 2025; 26(1):132. https://doi.org/10.3390/ijms26010132

Chicago/Turabian Style

Huang, Bin, Wenyin Huang, Zhenyu Liu, Yixuan Peng, Yanshu Qu, Wencai Zhou, Jianjian Huang, Huili Shu, and Qiang Wen. 2025. "Cytological, Physiological, and Transcriptome Analysis of Leaf-Yellowing Mutant in Camellia chekiangoleosa" International Journal of Molecular Sciences 26, no. 1: 132. https://doi.org/10.3390/ijms26010132

APA Style

Huang, B., Huang, W., Liu, Z., Peng, Y., Qu, Y., Zhou, W., Huang, J., Shu, H., & Wen, Q. (2025). Cytological, Physiological, and Transcriptome Analysis of Leaf-Yellowing Mutant in Camellia chekiangoleosa. International Journal of Molecular Sciences, 26(1), 132. https://doi.org/10.3390/ijms26010132

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop