In Vitro Cross-Linking MS Reveals SMG1–UPF2–SMG7 Assembly as Molecular Partners within the NMD Surveillance
Abstract
:1. Introduction
2. Results and Discussions
2.1. Inter- and Intramolecular Interactions between UPF2, SMG1, and SMG7
2.2. Effects of Cancer-Derived Mutations on the Identified Protein Cross-Links
3. Materials and Methods
3.1. Identification of Protein–Protein Networks within the NMD Machinery
3.2. Homology Modelling and Mutational Landscape of Cross-Linked Genes
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Padariya, M.; Vojtesek, B.; Hupp, T.; Kalathiya, U. In Vitro Cross-Linking MS Reveals SMG1–UPF2–SMG7 Assembly as Molecular Partners within the NMD Surveillance. Int. J. Mol. Sci. 2024, 25, 3182. https://doi.org/10.3390/ijms25063182
Padariya M, Vojtesek B, Hupp T, Kalathiya U. In Vitro Cross-Linking MS Reveals SMG1–UPF2–SMG7 Assembly as Molecular Partners within the NMD Surveillance. International Journal of Molecular Sciences. 2024; 25(6):3182. https://doi.org/10.3390/ijms25063182
Chicago/Turabian StylePadariya, Monikaben, Borivoj Vojtesek, Ted Hupp, and Umesh Kalathiya. 2024. "In Vitro Cross-Linking MS Reveals SMG1–UPF2–SMG7 Assembly as Molecular Partners within the NMD Surveillance" International Journal of Molecular Sciences 25, no. 6: 3182. https://doi.org/10.3390/ijms25063182