Phenotypic Investigation and RNA-seq of KN1 Involved in Leaf Angle Formation in Maize (Zea mays L.)
Abstract
:1. Introduction
2. Results
2.1. Identification and Basic Bioinformatics Analysis of KN1
2.2. Identification of Phenotype and Grain Characteristics of Mutant kn1
2.3. Spraying of Exogenous Phytohormones and Determination of Optimal Concentration
2.4. Transcriptome Sequencing (RNA-seq) Statistics and Transcriptomic Profile of B73 and kn1
2.5. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Enrichment Analysis of LA Mutant kn1
2.6. GO and KEGG Enrichment Analysis of kn1 and B73 Treated with Exogenous ABA and IAA
2.7. qRT-PCR Verification of Differentially Expressed Genes
2.8. Identification of Potential Hub Genes Involved in Regulation of LA via Weighted Gene Co-Expression Network Analysis (WGCNA)
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Planting Circumstances
4.2. Analysis of kn1 Mutant Phenotype
4.3. Bioinformatics-Related Analysis of KN1
4.4. Identification of Homozygous Mutant kn1
4.5. Expression Pattern Analysis of KN1
4.6. Determination of the Optimal Concentration of Exogenous Phytohormones
4.7. Transcriptomic Profiles and Data Analysis
4.8. Reliability Verification of RNA-seq via qRT-PCR
4.9. Weighted Gene Co-Expression Network Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Name of Cis-Acting Element | Nucleotide Sequence | Function Annotation |
---|---|---|---|
KN1 | TCA-element | TCAGAAGAGG | Salicylic acid-responsive |
CGTCA-motif | CGTCA | Methyl jasmonate-responsive | |
TGACG-motif | TGACG | Methyl jasmonate-responsive | |
CAAT-box | TGCCAAC/CAAAT | Cis-acting element shared by promoters and enhancers | |
MBS | CAACTG | Drought response-related MYB binding sites | |
ABRE | AACCCGG | Abscisic acid-responsive | |
TATA-box | TATA | Core promoter element | |
CCAAT-box | CAACGG | MYBHv1 binding site | |
O2-site | GATGATGTGG | Zein metabolism-related cis-acting element | |
A-box | CCGTCC | Cis-acting regulatory element | |
CAT-box | GCCACT | Meristem-related cis-acting element | |
TGA-element | AACGAC | Auxin-responsive | |
GARE-motif | TCTGTTG | Gibberellins-responsive | |
P-box | CCTTTTG | Gibberellins-responsive | |
TCCC-motif | TCTCCCT | Light-responsive | |
GATA-motif | AAGGATAAGG | Light-responsive | |
Sp1 | GGGCGG | Light-responsive |
Field Phenotype and Grain Traits | B73 | kn1 |
---|---|---|
Plant height (cm) | 250.39 ± 0.87 a | 203.00 ± 1.15 b |
Ear height (cm) | 108.78 ± 1.95 a | 93.67 ± 0.73 b |
Stem diameter (mm) | 22.29 ± 0.38 a | 21.3 ± 0.66 a |
Chlorophyll content | 50.14 ± 1.64 a | 52.5 ± 0.45 a |
Leaf length (cm) | 78.54 ± 2.08 a | 79.06 ± 0.97 a |
Leaf width (cm) | 9.23 ± 0.18 a | 8.30 ± 0.17 b |
Leaf area size (cm2) | 545.56 ± 23.84 a | 492.74 ± 14.17 a |
Leaf direction value | 5.35 ± 0.28 a | 6.10 ± 0.38 a |
Ear weight (g) | 81.52 ± 6.98 a | 64.82 ± 0.95 b |
Ear length (cm) | 10.87 ± 0.49 a | 10.73 ± 0.23 a |
Ear diameter (cm) | 3.97 ± 0.32 a | 4.13 ± 0.09 a |
100-kernel weight (g) | 16.61 ± 1.42 b | 21.65 ± 0.37 a |
Quantity of rows | 26.67 ± 1.15 a | 16.67 ± 0.67 b |
Ear rows | 16.67 ± 1.15 a | 15.33 ± 0.67 a |
Cob weight (g) | 14.86 ± 1.80 a | 17.20 ± 0.49 a |
Cob diameter (cm) | 2.77 ± 0.21 a | 3.10 ± 0.03 a |
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Wu, Y.; Zhang, Y.; Zhuang, Z.; Ji, X.; Bian, J.; Xian, J.; Wang, Y.; Peng, Y. Phenotypic Investigation and RNA-seq of KN1 Involved in Leaf Angle Formation in Maize (Zea mays L.). Int. J. Mol. Sci. 2024, 25, 3180. https://doi.org/10.3390/ijms25063180
Wu Y, Zhang Y, Zhuang Z, Ji X, Bian J, Xian J, Wang Y, Peng Y. Phenotypic Investigation and RNA-seq of KN1 Involved in Leaf Angle Formation in Maize (Zea mays L.). International Journal of Molecular Sciences. 2024; 25(6):3180. https://doi.org/10.3390/ijms25063180
Chicago/Turabian StyleWu, Yuanming, Yunfang Zhang, Zelong Zhuang, Xiangzhuo Ji, Jianwen Bian, Jinhong Xian, Yinxia Wang, and Yunling Peng. 2024. "Phenotypic Investigation and RNA-seq of KN1 Involved in Leaf Angle Formation in Maize (Zea mays L.)" International Journal of Molecular Sciences 25, no. 6: 3180. https://doi.org/10.3390/ijms25063180