Identification and Expression Patterns of WOX Transcription Factors under Abiotic Stresses in Pinus massoniana
Abstract
:1. Introduction
2. Results
2.1. Identification and Cloning of WOX Genes in P. massoniana
2.2. Phylogenetic Analysis of PmWOX
2.3. Protein Domain Analysis of PmWOX Proteins
2.4. Analysis of the Transcriptional Profiles of PmWOX Genes under Drought Stress
2.5. Expression Patterns of PmWOX Genes in Different Tissues
2.6. Expression Patterns of PmWOX Genes under Different Treatments
2.7. Transcriptional Activation Activity
3. Discussion
4. Materials and Methods
4.1. Identification and Cloning of WOX Genes in P. massoniana
4.2. Bioinformatics and Phylogenetic Analysis of PmWOX Proteins
4.3. RNA-Seq Data Analysis under Drought Stress
4.4. Plant Materials and Abiotic Stress Treatments
4.5. RNA Extraction and qRT-PCR Analysis
4.6. Transcriptional-Activation Activity Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Protein ID | Prediction of Subcellular Localization | Amino Acid Number (aa) | Protein Molecular Weight (KD) | Isoelectric Point | Instability Index | Grand Average of Hydropathicity |
---|---|---|---|---|---|---|
PmWOX1 | nucleus | 354 | 40.03 | 6.21 | 47.92 | −0.857 |
PmWOX2 | nucleus | 366 | 40.68 | 5.92 | 43.82 | −0.783 |
PmWOX3 | nucleus | 482 | 53.15 | 7.11 | 57.57 | −0.654 |
PmWOX4 | nucleus | 294 | 33.23 | 6.61 | 52.45 | −0.657 |
PmWOX5 | nucleus | 171 | 19.64 | 8.80 | 38.32 | −0.753 |
PmWOX6 | nucleus | 117 | 13.64 | 10.26 | 58.31 | −0.997 |
PmWOX7 | nucleus | 80 | 9.71 | 9.35 | 52.38 | −1.290 |
PmWOX8 | nucleus | 110 | 12.75 | 6.47 | 55.07 | −1.286 |
PmWOX9 | nucleus | 189 | 21.95 | 8.19 | 63.33 | −0.695 |
PmWUS | nucleus | 215 | 24.88 | 8.05 | 63.64 | −0.926 |
PmWOXX | nucleus | 168 | 18.83 | 5.45 | 65.06 | −0.656 |
Motif | Sequence | Number of Amino Acids in the Motif |
---|---|---|
1 | ILEAJYDQGIRTPSKEQIKEITAELSQYGKIEGTNVFYWFQNRKARERRK | 50 |
2 | QVMTEEQLETLRRQICVYSTICSQLVEMHRAMSQQ | 35 |
3 | FRPSARTRWNPTPEQL | 16 |
4 | DGKQWZVPVGVVDVRRMFGENAVLLDSRGHMVPTNDMGMSFHPLQGSEGY | 50 |
5 | GESEVDTDLESPKEKKVKMDH | 21 |
6 | TLZLFPVHPE | 10 |
7 | CCWGGC | 10 |
8 | RMLYDL | 6 |
9 | CEEAFCC | 7 |
10 | CVEEYDC | 7 |
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Wang, D.; Qiu, Z.; Xu, T.; Yao, S.; Zhang, M.; Cheng, X.; Zhao, Y.; Ji, K. Identification and Expression Patterns of WOX Transcription Factors under Abiotic Stresses in Pinus massoniana. Int. J. Mol. Sci. 2024, 25, 1627. https://doi.org/10.3390/ijms25031627
Wang D, Qiu Z, Xu T, Yao S, Zhang M, Cheng X, Zhao Y, Ji K. Identification and Expression Patterns of WOX Transcription Factors under Abiotic Stresses in Pinus massoniana. International Journal of Molecular Sciences. 2024; 25(3):1627. https://doi.org/10.3390/ijms25031627
Chicago/Turabian StyleWang, Dengbao, Zimo Qiu, Tao Xu, Sheng Yao, Mengyang Zhang, Xiang Cheng, Yulu Zhao, and Kongshu Ji. 2024. "Identification and Expression Patterns of WOX Transcription Factors under Abiotic Stresses in Pinus massoniana" International Journal of Molecular Sciences 25, no. 3: 1627. https://doi.org/10.3390/ijms25031627