Wnt/β-Catenin Signaling Regulates Yap/Taz Activity during Embryonic Development in Zebrafish
Abstract
1. Introduction
2. Results
2.1. Wnt/β-Catenin and Hippo-Yap/Taz Pathways Are Characterized by an Overlapping Spatial Expression during Zebrafish Development
2.2. Pharmacological Inhibition of the Wnt/β-Catenin Pathway Reduces Yap/Taz Activity during Zebrafish Embryonic Development
2.3. Genetic Inhibition of the Wnt/β-Catenin Pathway Reduces Yap/Taz Activity during Zebrafish Embryonic Development
2.4. Pharmacological Inhibition of β-Catenin Kinase GSK3 during Zebrafish Embryonic Development Increases Yap/Taz Activity
2.5. Activation of the Wnt/β-Catenin Pathway through Apc Depletion Increases Yap/Taz Activity during Zebrafish Development
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Chemical Treatments
4.3. Fluorescence Image Acquisition and Analysis
4.4. Whole-Mount In Situ Hybridization (WISH) and Quantification
4.5. Alcian Blue Staining
4.6. Heat-Shock Treatments
4.7. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Astone, M.; Tesoriero, C.; Schiavone, M.; Facchinello, N.; Tiso, N.; Argenton, F.; Vettori, A. Wnt/β-Catenin Signaling Regulates Yap/Taz Activity during Embryonic Development in Zebrafish. Int. J. Mol. Sci. 2024, 25, 10005. https://doi.org/10.3390/ijms251810005
Astone M, Tesoriero C, Schiavone M, Facchinello N, Tiso N, Argenton F, Vettori A. Wnt/β-Catenin Signaling Regulates Yap/Taz Activity during Embryonic Development in Zebrafish. International Journal of Molecular Sciences. 2024; 25(18):10005. https://doi.org/10.3390/ijms251810005
Chicago/Turabian StyleAstone, Matteo, Chiara Tesoriero, Marco Schiavone, Nicola Facchinello, Natascia Tiso, Francesco Argenton, and Andrea Vettori. 2024. "Wnt/β-Catenin Signaling Regulates Yap/Taz Activity during Embryonic Development in Zebrafish" International Journal of Molecular Sciences 25, no. 18: 10005. https://doi.org/10.3390/ijms251810005
APA StyleAstone, M., Tesoriero, C., Schiavone, M., Facchinello, N., Tiso, N., Argenton, F., & Vettori, A. (2024). Wnt/β-Catenin Signaling Regulates Yap/Taz Activity during Embryonic Development in Zebrafish. International Journal of Molecular Sciences, 25(18), 10005. https://doi.org/10.3390/ijms251810005