Selenium Discrepancies in Fetal Bovine Serum: Impact on Cellular Selenoprotein Expression
Abstract
:1. Introduction
2. Results
2.1. Selenium Levels in FBS Greatly Vary from Suppliers and Lot Number
2.2. Impact of FBS Selenium Levels on the Expression of GPX1, GPX4, and TXNRD1 in HEK293 Cells
2.3. Impact of FBS Selenium Levels on the GPX and TXNRD Enzymatic Activities in HEK293 Cells
2.4. Impact of FBS Selenium Levels on the UGA Recoding Activities as Measured by Luciferase Reporter Assay
3. Discussion
3.1. Selenium Levels in FBS Greatly Vary from Suppliers and Lot Numbers
3.2. Selenium Levels in FBS Correlates with GPX1 and GPX4 Expression in Cells
3.3. Selenium Bioavailability from FBS Is Mostly Supported by SELENOP
4. Materials and Methods
4.1. Materials
4.2. Triple Quadrupole ICP-MS Analysis
4.3. Cell Culture
4.4. Protein Extraction and Analysis by Western Blot
4.5. GPX and TXNRD Enzymatic Assays
4.6. Luciferase Assays
4.7. Graphical Illustrations and Data Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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FBS | Supplier (Distributor) | Supplier Location (City, State, Country) | Reference | Batch | Origin |
---|---|---|---|---|---|
#1 | Gibco (Thermo Fisher Scientific) | Waltham, MA, USA | 10-437-028 | 2230743RP | Mexico |
#2 | Gibco (Thermo Fisher Scientific) | Waltham, MA, USA | 10-437-028 | 2286113RP | Mexico |
#3 | Eurobio Scientific | Les Ulis, France | CVFSVF00-01 | S73136 | South America |
#4 | Avantor (VWR) | Rosny-sous-Bois cedex, France | 97068-085 | 141K20 | USA |
#5 | Biosera | Cholet, France | FB-1280/500 | S00AV10004 | EU |
#6 | Biowest | Nuaillé, France | S1400-500 | S00F610003 | French |
#7 | Biowest | Nuaillé, France | S1810-500 | S00H810001 | South America |
#8 | Merck | Darmstadt, Germany | K7524 | BCBV8085 | Non-USA origin |
#9 | Eurobio Scientific | Les Ulis, France | CVFSVF00-0U | S72121-1218 | South America |
#10 | Cytiva Hyclone (Thermo Fisher Scientific) | Waltham, MA, USA | SV30160.02 | RF20200006 | South America |
#11 | Seradigm (VWR) | Rosny-sous-Bois cedex, France | 3100-050 | 48B16 | USA |
#12 | Gibco (Thermo Fisher Scientific) | Waltham, MA, USA | 10270-106 | 41Q5484K | Brazil |
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Parant, F.; Mure, F.; Maurin, J.; Beauvilliers, L.; Chorfa, C.; El Jamali, C.; Ohlmann, T.; Chavatte, L. Selenium Discrepancies in Fetal Bovine Serum: Impact on Cellular Selenoprotein Expression. Int. J. Mol. Sci. 2024, 25, 7261. https://doi.org/10.3390/ijms25137261
Parant F, Mure F, Maurin J, Beauvilliers L, Chorfa C, El Jamali C, Ohlmann T, Chavatte L. Selenium Discrepancies in Fetal Bovine Serum: Impact on Cellular Selenoprotein Expression. International Journal of Molecular Sciences. 2024; 25(13):7261. https://doi.org/10.3390/ijms25137261
Chicago/Turabian StyleParant, François, Fabrice Mure, Julien Maurin, Léana Beauvilliers, Chaïma Chorfa, Chaymae El Jamali, Théophile Ohlmann, and Laurent Chavatte. 2024. "Selenium Discrepancies in Fetal Bovine Serum: Impact on Cellular Selenoprotein Expression" International Journal of Molecular Sciences 25, no. 13: 7261. https://doi.org/10.3390/ijms25137261