First Insight into the Degradome of Aspergillus ochraceus: Novel Secreted Peptidases and Their Inhibitors

Shestakova, Anna; Fatkulin, Artem; Surkova, Daria; Osmolovskiy, Alexander; Popova, Elizaveta

doi:10.3390/ijms25137121

Open AccessArticle

First Insight into the Degradome of Aspergillus ochraceus: Novel Secreted Peptidases and Their Inhibitors

by

Anna Shestakova

¹

,

Artem Fatkulin

²

,

Daria Surkova

¹,

Alexander Osmolovskiy

¹

and

Elizaveta Popova

^1,*

¹

Department of Microbiology, Lomonosov MSU, Moscow 119234, Russia

²

Laboratory of Molecular Physiology, HSE University, Moscow 101000, Russia

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2024, 25(13), 7121; https://doi.org/10.3390/ijms25137121

Submission received: 9 May 2024 / Revised: 7 June 2024 / Accepted: 14 June 2024 / Published: 28 June 2024

(This article belongs to the Special Issue Advances in Proteolysis and Proteolytic Enzymes)

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Abstract

Aspergillus fungi constitute a pivotal element within ecosystems, serving as both contributors of biologically active compounds and harboring the potential to cause various diseases across living organisms. The organism’s proteolytic enzyme complex, termed the degradome, acts as an intermediary in its dynamic interaction with the surrounding environment. Using techniques such as genome and transcriptome sequencing, alongside protein prediction methodologies, we identified putative extracellular peptidases within Aspergillus ochraceus VKM-F4104D. Following manual annotation procedures, a total of 11 aspartic, 2 cysteine, 2 glutamic, 21 serine, 1 threonine, and 21 metallopeptidases were attributed to the extracellular degradome of A. ochraceus VKM-F4104D. Among them are enzymes with promising applications in biotechnology, potential targets and agents for antifungal therapy, and microbial antagonism factors. Thus, additional functionalities of the extracellular degradome, extending beyond mere protein substrate digestion for nutritional purposes, were demonstrated.

Keywords: peptidases; proteases; degradome; MEROPS; HMMER; Aspergillus ochraceus; microscopic fungi; pathogenic fungi; antifungal therapy; substrate specificity

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MDPI and ACS Style

Shestakova, A.; Fatkulin, A.; Surkova, D.; Osmolovskiy, A.; Popova, E. First Insight into the Degradome of Aspergillus ochraceus: Novel Secreted Peptidases and Their Inhibitors. Int. J. Mol. Sci. 2024, 25, 7121. https://doi.org/10.3390/ijms25137121

AMA Style

Shestakova A, Fatkulin A, Surkova D, Osmolovskiy A, Popova E. First Insight into the Degradome of Aspergillus ochraceus: Novel Secreted Peptidases and Their Inhibitors. International Journal of Molecular Sciences. 2024; 25(13):7121. https://doi.org/10.3390/ijms25137121

Chicago/Turabian Style

Shestakova, Anna, Artem Fatkulin, Daria Surkova, Alexander Osmolovskiy, and Elizaveta Popova. 2024. "First Insight into the Degradome of Aspergillus ochraceus: Novel Secreted Peptidases and Their Inhibitors" International Journal of Molecular Sciences 25, no. 13: 7121. https://doi.org/10.3390/ijms25137121

APA Style

Shestakova, A., Fatkulin, A., Surkova, D., Osmolovskiy, A., & Popova, E. (2024). First Insight into the Degradome of Aspergillus ochraceus: Novel Secreted Peptidases and Their Inhibitors. International Journal of Molecular Sciences, 25(13), 7121. https://doi.org/10.3390/ijms25137121

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First Insight into the Degradome of Aspergillus ochraceus: Novel Secreted Peptidases and Their Inhibitors

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