The Anti-Atopic Dermatitis Effects of Mentha arvensis Essential Oil Are Involved in the Inhibition of the NLRP3 Inflammasome in DNCB-Challenged Atopic Dermatitis BALB/c Mice
Abstract
:1. Introduction
2. Results
2.1. Chemical Analysis of MAEO
2.2. The Effects of MAEO on NLRP3 Inflammasome Formation in BMDMs
2.3. The Effects of MAEO on DNCB-Induced AD-like Clinical Symptoms in BALB/c Mice
2.4. The Effects of MAEO on NLRP3 Inflammasome Formation in Macrophages of DNCB-Induced AD-like Murine Dorsal Skin
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Mentha arvensis Essential Oil
4.3. Gas Chromatography-Mass Spectrometry
4.4. Animals
4.5. Isolation and Differentiation of Mouse BMDMs
4.6. Enzyme-Linked Immunosorbent Assay (ELISA)
4.7. Western Blot Analysis
4.8. DNCB-Induced AD Mice
4.9. Histological Observation
4.10. Double-Immunofluorescence
4.11. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Compound Name | Retention Time | Area (%) | |
---|---|---|---|
1 | α-Pinene | 8.288 | 0.43 |
2 | β-Phellandrene | 9.731 | 0.28 |
3 | β-Pinene | 9.898 | 1.12 |
4 | 3-Octanol | 10.659 | 0.36 |
5 | Limonene | 11.751 | 3.05 |
6 | Eucalyptol (Cineole) | 11.848 | 0.06 |
7 | β-Ocimene | 11.988 | 0.32 |
8 | trans-β-Ocimene | 12.336 | 0.23 |
9 | Linalool | 14.147 | 0.26 |
10 | Isopulegol | 15.772 | 0.22 |
11 | Menthone | 16.113 | 25.98 |
12 | Neoisomenthol acetate | 16.424 | 2.46 |
13 | Isopulegone | 16.585 | 0.23 |
14 | Menthol | 16.897 | 35.67 |
15 | 2,3-Dimethyl-2-cyclopenten-1-one | 18.193 | 0.11 |
16 | Hexen-3-yl Valerate | 18.295 | 0.21 |
17 | Menthone | 18.455 | 0.21 |
18 | Piperitone oxide | 18.830 | 0.44 |
19 | Piperitone | 18.964 | 9.44 |
20 | γ-Diosphenol | 19.199 | 0.02 |
21 | Neoisomenthol acetate | 19.922 | 8.74 |
22 | Diosphenol | 20.147 | 0.10 |
23 | β-Bourbonene | 22.404 | 0.15 |
24 | β-Elemene | 22.535 | 0.05 |
25 | Jasmone | 22.674 | 0.04 |
26 | p-Menthane-1,2,3-triol | 23.069 | 1.93 |
27 | Caryophyllene | 23.356 | 0.89 |
28 | p-Menthane-1,2,3-triol | 23.519 | 0.12 |
29 | Dihydroumbellulone | 23.605 | 0.45 |
30 | Piperitone | 24.075 | 3.85 |
31 | α-Caryophyllene | 24.267 | 0.12 |
32 | Bicyclosesquiphellandrene | 24.427 | 0.31 |
33 | β-Cubebene | 24.905 | 0.84 |
34 | Rose butanoate | 24.992 | 0.03 |
35 | Elixene | 25.262 | 0.05 |
36 | Butylated Hydroxytoluene (BHT) | 25.384 | 0.09 |
37 | γ-Cadinene | 25.679 | 0.04 |
38 | β-Cadinene | 25.783 | 0.07 |
39 | Calamenene | 25.860 | 0.09 |
40 | α-Muuroladiene | 26.241 | 0.05 |
41 | Germacrene-D-4-ol | 27.211 | 0.08 |
42 | Cubenol | 28.114 | 0.11 |
43 | α-Cadinol | 29.026 | 0.08 |
44 | Phytone | 32.858 | 0.04 |
Total | 99.42 |
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Kim, S.-Y.; Sapkota, A.; Bae, Y.J.; Choi, S.-H.; Bae, H.J.; Kim, H.-J.; Cho, Y.E.; Choi, Y.-Y.; An, J.-Y.; Cho, S.-Y.; et al. The Anti-Atopic Dermatitis Effects of Mentha arvensis Essential Oil Are Involved in the Inhibition of the NLRP3 Inflammasome in DNCB-Challenged Atopic Dermatitis BALB/c Mice. Int. J. Mol. Sci. 2023, 24, 7720. https://doi.org/10.3390/ijms24097720
Kim S-Y, Sapkota A, Bae YJ, Choi S-H, Bae HJ, Kim H-J, Cho YE, Choi Y-Y, An J-Y, Cho S-Y, et al. The Anti-Atopic Dermatitis Effects of Mentha arvensis Essential Oil Are Involved in the Inhibition of the NLRP3 Inflammasome in DNCB-Challenged Atopic Dermatitis BALB/c Mice. International Journal of Molecular Sciences. 2023; 24(9):7720. https://doi.org/10.3390/ijms24097720
Chicago/Turabian StyleKim, So-Yeon, Arjun Sapkota, Young Joo Bae, Seung-Hyuk Choi, Ho Jung Bae, Hyun-Jeong Kim, Ye Eun Cho, Yu-Yeong Choi, Ju-Yeon An, So-Young Cho, and et al. 2023. "The Anti-Atopic Dermatitis Effects of Mentha arvensis Essential Oil Are Involved in the Inhibition of the NLRP3 Inflammasome in DNCB-Challenged Atopic Dermatitis BALB/c Mice" International Journal of Molecular Sciences 24, no. 9: 7720. https://doi.org/10.3390/ijms24097720