The RING-Finger Protein NbRFP1 Contributes to Regulating the Host Hypersensitive Response Induced by Oat Dwarf Virus RepA
Abstract
:1. Introduction
2. Results
2.1. The Isolation and Sequence Analysis of NbRFP1
2.2. Expression Pattern and Subcellular Localization of NbRFP1
2.3. Silencing of NbRFP1 Delays ODV RepA-Induced HR
2.4. The Overexpression of NbRFP1 Enhances ODV RepA-Induced HR
2.5. ODV RepA Interacts with NbRFP1
2.6. The RING-Finger Domain in NbRFP1 Was Involved in the Interaction with RepA and Positively Regulated RepA-Induced HR
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. RNA Extraction, RT-PCR, and qPCR Analyses
4.3. Subcellular Localization
4.4. BiFC Assay
4.5. Protein Extraction and Western Blotting Analysis
4.6. CoIP Assay
4.7. Silencing of Endogenous NbRFP1
4.8. Transient Co-Expression of RepA and NbRFP1
4.9. Phylogenetic Analysis
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liang, Y.; Wang, Z.; Wang, Q.; Zhou, X.; Qian, Y. The RING-Finger Protein NbRFP1 Contributes to Regulating the Host Hypersensitive Response Induced by Oat Dwarf Virus RepA. Int. J. Mol. Sci. 2023, 24, 7697. https://doi.org/10.3390/ijms24097697
Liang Y, Wang Z, Wang Q, Zhou X, Qian Y. The RING-Finger Protein NbRFP1 Contributes to Regulating the Host Hypersensitive Response Induced by Oat Dwarf Virus RepA. International Journal of Molecular Sciences. 2023; 24(9):7697. https://doi.org/10.3390/ijms24097697
Chicago/Turabian StyleLiang, Yanqing, Zhanqi Wang, Qian Wang, Xueping Zhou, and Yajuan Qian. 2023. "The RING-Finger Protein NbRFP1 Contributes to Regulating the Host Hypersensitive Response Induced by Oat Dwarf Virus RepA" International Journal of Molecular Sciences 24, no. 9: 7697. https://doi.org/10.3390/ijms24097697