Nordalbergin Exerts Anti-Neuroinflammatory Effects by Attenuating MAPK Signaling Pathway, NLRP3 Inflammasome Activation and ROS Production in LPS-Stimulated BV2 Microglia
Abstract
:1. Introduction
2. Results
2.1. Nordalbergin Decreases the Secretion of NO and the Expression of iNOS and COX-2 by LPS-Stimulated BV2 Cells
2.2. Nordalbergin Suppresses LPS-Induced Pro-Inflammatory Cytokine Production by Murine Microglial BV2 Cells
2.3. Nordalbergin Decreases the Phosphorylation of ERK, JNK and p38 MAPK by LPS-Stimulated Microglial BV2 Cells
2.4. Nordalbergin Inhibits the Secretion of IL-1β and Represses the Activation of NLRP3 Inflammasome in LPS-Stimulated BV2 Cells
2.5. Nordalbergin Decreases Both Intracellular and Mitochondrial ROS Production by LPS-Stimulated BV2 Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. NO Assay
4.3. MTT Assay for Cell Viability
4.4. ELISA
4.5. Western Blot Analysis
4.6. Flow Cytometry
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lo, J.; Wu, H.-E.; Liu, C.-C.; Chang, K.-C.; Lee, P.-Y.; Liu, P.-L.; Huang, S.-P.; Wu, P.-C.; Lin, T.-C.; Lai, Y.-H.; et al. Nordalbergin Exerts Anti-Neuroinflammatory Effects by Attenuating MAPK Signaling Pathway, NLRP3 Inflammasome Activation and ROS Production in LPS-Stimulated BV2 Microglia. Int. J. Mol. Sci. 2023, 24, 7300. https://doi.org/10.3390/ijms24087300
Lo J, Wu H-E, Liu C-C, Chang K-C, Lee P-Y, Liu P-L, Huang S-P, Wu P-C, Lin T-C, Lai Y-H, et al. Nordalbergin Exerts Anti-Neuroinflammatory Effects by Attenuating MAPK Signaling Pathway, NLRP3 Inflammasome Activation and ROS Production in LPS-Stimulated BV2 Microglia. International Journal of Molecular Sciences. 2023; 24(8):7300. https://doi.org/10.3390/ijms24087300
Chicago/Turabian StyleLo, Jung, Hsin-En Wu, Ching-Chih Liu, Kun-Che Chang, Po-Yen Lee, Po-Len Liu, Shu-Pin Huang, Pei-Chang Wu, Tzu-Chieh Lin, Yu-Hung Lai, and et al. 2023. "Nordalbergin Exerts Anti-Neuroinflammatory Effects by Attenuating MAPK Signaling Pathway, NLRP3 Inflammasome Activation and ROS Production in LPS-Stimulated BV2 Microglia" International Journal of Molecular Sciences 24, no. 8: 7300. https://doi.org/10.3390/ijms24087300