Osteogenic Differentiation Effect of Human Periodontal Ligament Stem-Cell Initial Cell Density on Autologous Cells and Human Bone Marrow Stromal Cells
Abstract
:1. Introduction
2. Results
2.1. hPDLSC Proliferation as a Function of Culture Density
2.2. Effect of Cell Density on the Expression of OPG, RUNX2, and OPG/RANKL during Osteogenesis of hPDLSCs
2.3. hPDLSCs with Different Initial Culture Densities Enable High-Yield Production of Exosomes
2.4. Osteogenic Effects of hPDLSC Exosomes Derived from Cultures with Different Initial Cell Densities on hBMSCs
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Identification of hPDLSCs and hBMSCs
4.2. Cell Proliferation Test
4.3. Isolation of Exosomes
4.4. Characteristics of Exosomes
4.5. Alizarin Red Staining Test
4.6. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR)
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene (Human) | Forward Primer | Reverse Primer |
---|---|---|
OPG | 5′-GAGTCCGATCCAGCCAAGA-3′ | 5′-GTACGGCGGAAACTCACAG-3′ |
OCN | 5′-TAGTGAAGAGACCCAGGCGCT-3′ | 5′-ATAGGCCTCCTGAAAGCCGA-3′ |
RUNX2 | 5′-GGAATGCCTCTGCTGTTATGAA-3′ | 5′-GCTTCTGTCTGTGCCTTCTG-3′ |
Osterix | 5′-CTCCTGCGACTGCCCTAAT-3′ | 5′-CTCATCCGAACGAGTGAACCT-3′ |
RANKL | 5′-TCGCTGGGAAACAACACTG-3′ | 5′-GGGAAGGGAAAGGTAGATGC-3′ |
GAPDH | 5′-CTGGGCTACACTGAGCACC-3′ | 5′-AAGTGGTCGTTGAGGGCAATG-3′ |
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Wang, J.; Qiao, Q.; Sun, Y.; Yu, W.; Wang, J.; Zhu, M.; Yang, K.; Huang, X.; Bai, Y. Osteogenic Differentiation Effect of Human Periodontal Ligament Stem-Cell Initial Cell Density on Autologous Cells and Human Bone Marrow Stromal Cells. Int. J. Mol. Sci. 2023, 24, 7133. https://doi.org/10.3390/ijms24087133
Wang J, Qiao Q, Sun Y, Yu W, Wang J, Zhu M, Yang K, Huang X, Bai Y. Osteogenic Differentiation Effect of Human Periodontal Ligament Stem-Cell Initial Cell Density on Autologous Cells and Human Bone Marrow Stromal Cells. International Journal of Molecular Sciences. 2023; 24(8):7133. https://doi.org/10.3390/ijms24087133
Chicago/Turabian StyleWang, Jing, Qingchen Qiao, Yaxi Sun, Wenting Yu, Jiran Wang, Minjia Zhu, Kai Yang, Xiaofeng Huang, and Yuxing Bai. 2023. "Osteogenic Differentiation Effect of Human Periodontal Ligament Stem-Cell Initial Cell Density on Autologous Cells and Human Bone Marrow Stromal Cells" International Journal of Molecular Sciences 24, no. 8: 7133. https://doi.org/10.3390/ijms24087133