Rapid, Easy, and Reliable Identification of Nocardia sp. by MALDI-TOF Mass Spectrometry, VITEK®-MS IVD V3.2 Database, Using Direct Deposit
Abstract
:1. Introduction
2. Results
2.1. Nocardia Collection
2.2. Performance of VITEK®-MS for Nocardia Species Identification Using Direct Deposit
2.3. Discrepancy Analysis
3. Discussion
4. Materials and Methods
4.1. Nocardia Isolates
4.2. 16S rRNA/hsp65 Sequencing-Based Nocardia Identification
4.3. VITEK®-MS IVD v3.2 Database Nocardia Identification
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Reference Identification | Number (%) of Isolates | |||||||
---|---|---|---|---|---|---|---|---|
Correct to Species Level | Correct to Complex Level | Incorrect | No. Identification | |||||
2 Wells/2 after the First Run | 1 Wells/2 after the First Run | >First Run (New Deposit) | Total | 1 Wells/2 after the First Run | Total | |||
N. asiatica (n = 4) | 4 (100.0) | |||||||
N. barduliensis N (n = 1) | 1 (100.0) | |||||||
N. brasilliensis (n = 9) | 4 (44.4) | 5 (55.6) | 9 (100.0) | |||||
N. cyriacigeorgica (n = 32) | 14 (43.7) | 10 (31.3) | 6 (18.7) | 30 (93.7) | 2 (6.3) | |||
N. elegans N (n = 1) | 1 (100.0) | 1 (100.0) | ||||||
N. farcinica (n = 31) | 16 (51.6) | 12 (38.7) | 2 (6.5) | 30 (96.8) | 1 (3.2) | |||
N. flavorosea N (n = 1) | 1 (100.0) | |||||||
N. gamkensis N (n = 8) | 1 (12.5) | 7 (87.5) | ||||||
N. gipuzkoensis N (n = 4) | 2 (50.0) | 2 (50.0) | ||||||
N. nova (n = 25) | 15 (60.0) | 7 (28.0) | 3 (12.0) | 25 (100.0) | ||||
N. otitidiscavarium (n = 1) | 1 (100.0) | 1 (100.0) | ||||||
N. pseudobrasiliensis (n = 1) | 1 (100.0) | 1 (100.0) | ||||||
N. puris N (n = 2) | 2 (100.0) | |||||||
N. shimofusensis N (n = 1) | 1 (100.0) | |||||||
N. spp N* (n = 3) | 3 (100.0) | |||||||
N. terpenica N (n = 1) | 1 (100.0) | |||||||
N. thailandica N (n = 1) | 1 (100.0) | |||||||
N. veterana (n = 2) | 1 (50.0) | 1 (50.0) | 2 (100.0) | |||||
N. wallacei (n = 6) | 1 (16.7) | 3 (50.0) | 2 (33.3) | 6 (100.0) | ||||
Total (n = 134) | 53 (39.6) | 37 (27.6) | 14 (10.4) | 104 (77.6) | 1 (0.7) | 1 (0.7) | 4 (3.0) | 25 (18.7) |
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Hodille, E.; Prudhomme, C.; Dumitrescu, O.; Benito, Y.; Dauwalder, O.; Lina, G. Rapid, Easy, and Reliable Identification of Nocardia sp. by MALDI-TOF Mass Spectrometry, VITEK®-MS IVD V3.2 Database, Using Direct Deposit. Int. J. Mol. Sci. 2023, 24, 5469. https://doi.org/10.3390/ijms24065469
Hodille E, Prudhomme C, Dumitrescu O, Benito Y, Dauwalder O, Lina G. Rapid, Easy, and Reliable Identification of Nocardia sp. by MALDI-TOF Mass Spectrometry, VITEK®-MS IVD V3.2 Database, Using Direct Deposit. International Journal of Molecular Sciences. 2023; 24(6):5469. https://doi.org/10.3390/ijms24065469
Chicago/Turabian StyleHodille, Elisabeth, Clémence Prudhomme, Oana Dumitrescu, Yvonne Benito, Olivier Dauwalder, and Gérard Lina. 2023. "Rapid, Easy, and Reliable Identification of Nocardia sp. by MALDI-TOF Mass Spectrometry, VITEK®-MS IVD V3.2 Database, Using Direct Deposit" International Journal of Molecular Sciences 24, no. 6: 5469. https://doi.org/10.3390/ijms24065469