Antimicrobial Activity of Sertraline on Listeria monocytogenes
Abstract
:1. Introduction
2. Results
2.1. Antimicrobial Activity of Sertraline
2.2. LIVE/DEAD Assay
2.3. Effect of Sertraline on the Bacterial Cell Membrane
2.4. Biofilm Formation Testing
2.5. FESEM Observation
2.6. Virulence Gene Expression
3. Discussion
4. Materials and Methods
4.1. Reagents and Bacteria
4.2. MIC and MBC
4.3. Growth and Viability Assays
4.4. Assessment of Membrane Integrity
4.5. Measurement of Intracellular ATP Concentrations
4.6. PHin Measurements
4.7. Biofilm Formation
4.8. Field Emission Scanning Electron Microscope (FESEM) Analysis
4.9. Virulence Gene Expression
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Strain | Source | MIC (μg/mL) | MBC (μg/mL) |
---|---|---|---|
L. monocytogenes ATCC 11915 | Standard strains | 32 | 64 |
L. monocytogenes 001 | Raw meat | 32 | 64 |
L. monocytogenes 69 | Frozen rice and noodle product | 16 | 64 |
L. monocytogenes 60 | Frozen rice and noodle product | 16 | 64 |
L. monocytogenes 01 | Frozen rice and noodle product | 16 | 64 |
L. monocytogenes 82 | Frozen rice and noodle product | 32 | 64 |
L. monocytogenes 44 | Frozen rice and noodle product | 32 | 64 |
L. monocytogenes 46 | Frozen rice and noodle product | 16 | 64 |
L. monocytogenes EGDe | Frozen rice and noodle product | 16 | 64 |
L. monocytogenes 10403s | Frozen rice and noodle product | 16 | 64 |
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Wang, Y.; Li, L.; Cai, P.; Olsen, R.H.; Peng, S.; Meng, H. Antimicrobial Activity of Sertraline on Listeria monocytogenes. Int. J. Mol. Sci. 2023, 24, 4678. https://doi.org/10.3390/ijms24054678
Wang Y, Li L, Cai P, Olsen RH, Peng S, Meng H. Antimicrobial Activity of Sertraline on Listeria monocytogenes. International Journal of Molecular Sciences. 2023; 24(5):4678. https://doi.org/10.3390/ijms24054678
Chicago/Turabian StyleWang, Yidi, Lili Li, Pingyao Cai, Rikke Heidemann Olsen, Shuai Peng, and Hecheng Meng. 2023. "Antimicrobial Activity of Sertraline on Listeria monocytogenes" International Journal of Molecular Sciences 24, no. 5: 4678. https://doi.org/10.3390/ijms24054678