Simple Detection and Culture of Circulating Tumor Cells from Colorectal Cancer Patients Using Poly(2-Methoxyethyl Acrylate)-Coated Plates
Abstract
:1. Introduction
2. Results
2.1. Adhesion of CRC Cell Lines to PMEA-Coated and Non-Coated Plates
2.2. Spike Test
2.3. Immunocytochemistry for EpCAM and Cytological Examination
2.4. Cell Culture
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Preparation of PMEA-Coated Chamber Slides
4.3. Adhesion Test
4.4. Spike Test
4.5. Immunostaining
4.6. Patient Background Data
4.7. Enrichment of CTCs in Blood Samples
4.8. Culture of CTCs
4.9. Definition of CTC
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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N = 41 | |
---|---|
Age: years; median (range) | 67 (2–80) |
Sex: Male/Female | 26/15 |
CEA ng/mL: median (range) | 30 (2–2739) |
CA19-9 U/mL: median (range) | 26.2 (0.4–4349.5) |
Location: Right/Left | 8/33 |
Stage II/III/IV | 1/2/38 |
Primary tumor Max diameter mm; median (range) | 45 (20–140) |
* Differentiation: tub/muc, por | 31/3 |
Liver metastasis: yes/no | 31/10 |
Lung metastasis: yes/no | 12/29 |
Peritoneal dissemination: yes/no | 7/34 |
Primary tumor resection: yes/no | 30/11 |
Treatment experience of chemotherapy or radiation: yes/no | 11/30 |
N = 41 | CTC (+) and/or Cell Growth (+), N = 23 | CTC (-) and Cell Growth (-) N = 18 | p Value | |
---|---|---|---|---|
Age: years; median (range) | 67 (25–80) | 67 (25–80) | 68 (43–80) | 0.64 |
Sex: Male/Female | 26/15 | 15/8 | 11/7 | 0.79 |
CEA ng/mL: median (range) | 30 (2–2739) | 21 (2–2739) | 33.5 (2–1956) | 0.89 |
CA19-9 U/mL: median (range) | 26.2 (0.4–4349.5) | 26.2 (0.4–2870.2) | 26.55 (0.4–4339.5) | 0.82 |
Location: Right/Left | 8/33 | 5/18 | 3/15 | 0.68 |
* Differentiation: tub/muc, por | 31/3 | 17/3 | 14/0 | 0.07 |
Primary tumor resection: yes/no | 30/11 | 17/6 | 13/5 | 0.90 |
Chemotherapy or radiation: yes/no | 11/30 | 3/20 | 8/10 | 0.02 |
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Nomura, M.; Yokoyama, Y.; Yoshimura, D.; Minagawa, Y.; Yamamoto, A.; Tanaka, Y.; Sekiguchi, N.; Marukawa, D.; Ichihara, M.; Itakura, H.; et al. Simple Detection and Culture of Circulating Tumor Cells from Colorectal Cancer Patients Using Poly(2-Methoxyethyl Acrylate)-Coated Plates. Int. J. Mol. Sci. 2023, 24, 3949. https://doi.org/10.3390/ijms24043949
Nomura M, Yokoyama Y, Yoshimura D, Minagawa Y, Yamamoto A, Tanaka Y, Sekiguchi N, Marukawa D, Ichihara M, Itakura H, et al. Simple Detection and Culture of Circulating Tumor Cells from Colorectal Cancer Patients Using Poly(2-Methoxyethyl Acrylate)-Coated Plates. International Journal of Molecular Sciences. 2023; 24(4):3949. https://doi.org/10.3390/ijms24043949
Chicago/Turabian StyleNomura, Masatoshi, Yuhki Yokoyama, Daishi Yoshimura, Yasuhisa Minagawa, Aki Yamamoto, Yukiko Tanaka, Naoko Sekiguchi, Daiki Marukawa, Momoko Ichihara, Hiroaki Itakura, and et al. 2023. "Simple Detection and Culture of Circulating Tumor Cells from Colorectal Cancer Patients Using Poly(2-Methoxyethyl Acrylate)-Coated Plates" International Journal of Molecular Sciences 24, no. 4: 3949. https://doi.org/10.3390/ijms24043949