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Article

Analysis of Genetic Relatedness between Gastric and Oral Helicobacter pylori in Patients with Early Gastric Cancer Using Multilocus Sequence Typing

by
Ryoko Nagata
1,
Hiroki Sato
2,
Shoji Takenaka
1,*,
Junji Yokoyama
2,
Shuji Terai
2,
Hitomi Mimuro
3 and
Yuichiro Noiri
1
1
Division of Cariology, Operative Dentistry and Endodontics, Faculty of Dentistry, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8514, Japan
2
Division of Gastroenterology and Hepatology, Faculty of Dentistry, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8510, Japan
3
Division of Genome-Wide Infectious Diseases, Research Center for GLOBAL and LOCAL Infectious Disease, Oita University, Oita 870-1192, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(3), 2211; https://doi.org/10.3390/ijms24032211
Submission received: 23 December 2022 / Revised: 13 January 2023 / Accepted: 19 January 2023 / Published: 22 January 2023
(This article belongs to the Special Issue Helicobacter pylori Cell Biology and Pathogenesis)
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Abstract

The oral cavity is the second most colonized site of Helicobacter pylori after the stomach. This study aimed to compare the genetic relatedness between gastric and oral H. pylori in Japanese patients with early gastric cancer through multilocus sequence typing (MLST) analysis using eight housekeeping genes. Gastric biopsy specimens and oral samples were collected from 21 patients with a fecal antigen test positive for H. pylori. The number of H. pylori allelic profiles ranged from zero to eight since the yield of DNA was small even when the nested PCR was performed. MLST analysis revealed that only one patient had a matching oral and gastric H. pylori genotype, suggesting that different genotypes of H. pylori inhabit the oral cavity and gastric mucosa. The phylogenetic analysis showed that oral H. pylori in six patients was similar to gastric H. pylori, implying that the two strains are related but not of the same origin, and those strains may be infected on separate occasions. It is necessary to establish a culture method for oral H. pylori to elucidate whether the oral cavity acts as the source of gastric infection, as our analysis was based on a limited number of allele sequences.
Keywords: Helicobacter pylori; genotypes; allele sequences; gastric mucosa; multilocus sequence typing Helicobacter pylori; genotypes; allele sequences; gastric mucosa; multilocus sequence typing

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MDPI and ACS Style

Nagata, R.; Sato, H.; Takenaka, S.; Yokoyama, J.; Terai, S.; Mimuro, H.; Noiri, Y. Analysis of Genetic Relatedness between Gastric and Oral Helicobacter pylori in Patients with Early Gastric Cancer Using Multilocus Sequence Typing. Int. J. Mol. Sci. 2023, 24, 2211. https://doi.org/10.3390/ijms24032211

AMA Style

Nagata R, Sato H, Takenaka S, Yokoyama J, Terai S, Mimuro H, Noiri Y. Analysis of Genetic Relatedness between Gastric and Oral Helicobacter pylori in Patients with Early Gastric Cancer Using Multilocus Sequence Typing. International Journal of Molecular Sciences. 2023; 24(3):2211. https://doi.org/10.3390/ijms24032211

Chicago/Turabian Style

Nagata, Ryoko, Hiroki Sato, Shoji Takenaka, Junji Yokoyama, Shuji Terai, Hitomi Mimuro, and Yuichiro Noiri. 2023. "Analysis of Genetic Relatedness between Gastric and Oral Helicobacter pylori in Patients with Early Gastric Cancer Using Multilocus Sequence Typing" International Journal of Molecular Sciences 24, no. 3: 2211. https://doi.org/10.3390/ijms24032211

APA Style

Nagata, R., Sato, H., Takenaka, S., Yokoyama, J., Terai, S., Mimuro, H., & Noiri, Y. (2023). Analysis of Genetic Relatedness between Gastric and Oral Helicobacter pylori in Patients with Early Gastric Cancer Using Multilocus Sequence Typing. International Journal of Molecular Sciences, 24(3), 2211. https://doi.org/10.3390/ijms24032211

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