MiR-221-3p/222-3p Cluster Expression in Human Adipose Tissue Is Related to Obesity and Type 2 Diabetes
Abstract
:1. Introduction
2. Results
2.1. Patient Characteristics
2.2. Human miR-221-3p and miR-222-3p Expression Profiles and Their Association with Obesity, T2D, and AT Depot
2.3. Human miR-221-3p and miR-222-3p Expression Profiles Were Analyzed in Relation to Obesity, T2D, and AT Depot, with Consideration of Sex-Based Differences
2.4. Mouse miR-221-3p and miR-222-3p Expression Profiles and Their Association with Obesity, T2D, and AT Depot
2.5. In Silico Analysis of miRNA-221-3p/222-3p Target Genes
2.6. Annotation Enrichment Analysis of miR-221-3p/222-3p Target Genes
2.7. Identification of miRNA Binding Sites within Target Genes
2.8. Gene Expression of ETS1, DVL2, and IL1RAP in Human VAT and SAT: Impact of Obesity, Glycemic Status, and Sex
3. Discussion
4. Materials and Methods
4.1. Patients, Study Design, and AT Collection
4.2. Laboratory Analysis
4.3. Generation of Diet-Induced Obese and Diabetic Mice
4.4. MiRNA Extraction and Real-Time Quantitative PCR (qPCR)
4.5. mRNA Isolation and qPCR
4.6. Bioinformatics Analysis
4.7. Statistical Analysis
5. Conclusions
6. Study Limitation
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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NG-NW (n = 11) | NG-OB (n = 11) | D-OB (n = 11) | |
---|---|---|---|
Age (years) | 41.32 ± 4.11 | 46.91 ± 3.55 | 43.91 ± 4.13 |
Sex (Man/Woman) | 5/6 | 5/6 | 5/6 |
BMI (kg/m2) | 22.48 ± 0.46 | 37.48 ± 1.39 * | 47.07 ± 2.52 *# |
Glucose (mg/dL) | 95.73 ± 4.05 | 89.91 ± 3.11 | 172.86 ± 22.73 *# |
HOMA-IR | 1.18 ± 1.56 | 2.28 ± 0.3 * | 7.90 ± 0.75 *# |
Triglycerides (mg/dL) | 72.23 ± 6.29 | 144.45 ± 27.39 * | 166.86 ± 20.45 *# |
Cholesterol (mg/dL) | 183.77 ± 5.15 | 202.05 ± 11.90 | 186.41 ± 14.99 |
HDL-c (mg/dL) | 62.5 ± 3.83 | 47.82 ± 5.39 * | 41.91 ± 2.65 *# |
LDL-c (mg/dL) | 111.36 ± 5.53 | 126.7 ± 9.54 | 115 ± 14.56 |
Validated Genes | Non-Validated Genes | Total | |
---|---|---|---|
miRNA | miRTarBase | miRWalk | |
miR-221 | 467 | 16 | 483 |
miR-222 | 444 | 13 | 457 |
TOTAL | 911 | 29 | 940 |
COMMON | 53 | 14 | 67 |
ENRICHMENT ANALYSIS | 7 | 17 | 24 |
SELECTED | 1 | 2 | 3 |
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Gentile, A.-M.; Lhamyani, S.; Mengual-Mesa, M.; García-Fuentes, E.; Bermúdez-Silva, F.-J.; Rojo-Martínez, G.; Clemente-Postigo, M.; Rodriguez-Cañete, A.; Olveira, G.; El Bekay, R. MiR-221-3p/222-3p Cluster Expression in Human Adipose Tissue Is Related to Obesity and Type 2 Diabetes. Int. J. Mol. Sci. 2023, 24, 17449. https://doi.org/10.3390/ijms242417449
Gentile A-M, Lhamyani S, Mengual-Mesa M, García-Fuentes E, Bermúdez-Silva F-J, Rojo-Martínez G, Clemente-Postigo M, Rodriguez-Cañete A, Olveira G, El Bekay R. MiR-221-3p/222-3p Cluster Expression in Human Adipose Tissue Is Related to Obesity and Type 2 Diabetes. International Journal of Molecular Sciences. 2023; 24(24):17449. https://doi.org/10.3390/ijms242417449
Chicago/Turabian StyleGentile, Adriana-Mariel, Said Lhamyani, María Mengual-Mesa, Eduardo García-Fuentes, Francisco-Javier Bermúdez-Silva, Gemma Rojo-Martínez, Mercedes Clemente-Postigo, Alberto Rodriguez-Cañete, Gabriel Olveira, and Rajaa El Bekay. 2023. "MiR-221-3p/222-3p Cluster Expression in Human Adipose Tissue Is Related to Obesity and Type 2 Diabetes" International Journal of Molecular Sciences 24, no. 24: 17449. https://doi.org/10.3390/ijms242417449