Antarctic Krill Oil from Euphausia superba Ameliorates Carrageenan-Induced Thrombosis in a Mouse Model
Abstract
:1. Introduction
2. Results
2.1. FJH-KO Attenuates Carrageenan-Induced Thrombosis in Mouse Tail
2.2. Effects of FJH-KO on Thrombosis Parameters
2.3. Effects of FJH-KO on Proinflammatory Cytokine Levels in Mouse Plasma
2.4. Effects of FJH-KO on Vascular Endothelial Dysfunction and Inflammation in Mouse Blood Vessels
2.5. Effects of FJH-KO on Carrageenan-Induced Tissue Thrombosis in Mice
2.6. Effects of FJH-KO on NO Production and eNOS Phosphorylation in Endothelial Cells
2.7. Effects of FJH-KO on TNF-α-Induced Monocyte Adhesion and Expression of Adhesion Molecules in Endothelial Cells
3. Discussion
4. Materials and Methods
4.1. Preparation of the Extract
4.2. Chemicals and Reagents
4.3. Carrageenan-Induced Thrombosis Mouse Model
4.4. Tail Bleeding Assay
4.5. Thrombus Length Measurement
4.6. Cell Culture and Treatment
4.7. Cell Viability and Cytotoxicity Assay
4.8. Real-Time PCR
4.9. Western Blotting
4.10. Hematoxylin and Eosin (H&E) Staining
4.11. ELISA
4.12. Measurement of NO Production
4.13. Monocyte Adhesion
4.14. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Sequences | |
---|---|---|
Mouse β-actin | F | AGCCATGTACGTAGCCATCC |
R | CTCTCAGCTGTGGTGGTGAA | |
Mouse eNOS | F | CGCAAGAGGAAGGAGTCTAGCA |
R | TCGAGCAAAGGCACAGAAGTGG | |
Mouse ICAM-1 | F | AAACCAGACCCTGGAACTGCAC |
R | GCCTGGCATTTCAGAGTCTGCT | |
Mouse VCAM-1 | F | GCTATGAGGATGGAAGACTCTGG |
R | ACTTGTGCAGCCACCTGAGATC | |
Mouse P-Selectin | F | AAGATGCCTGGCTACTGGACAC |
R | CAAGAGGCTGAACGCAGGTCAT | |
Mouse E-Selectin | F | GGACACCACAAATCCCAGTCTG |
R | TCGCAGGAGAACTCACAACTGG | |
Mouse MCP1 | F | GCTACAAGAGGATCACCAGCAG |
R | GTCTGGACCCATTCCTTCTTGG | |
Mouse TNF-α | F | GGTGCCTATGTCTCAGCCTCTT |
R | GCCATAGAACTGATGAGAGGGAG | |
Mouse IL-1β | F | TGGACCTTCCAGGATGAGGACA |
R | GTTCATCTCGGAGCCTGTAGTG | |
Mouse IL-6 | F | TACCACTTCACAAGTCGGAGGC |
R | CTGCAAGTGCATCATCGTTGTTC | |
Human GAPDH | F | GGGGAGCCAAAAGGGTCATC |
R | TGGTTCACACCCATGACGAA | |
Human ICAM-1 | F | GGCAGCGTAGGGTAAGGTT |
R | CCGGAAGGTGTATGAACTGA | |
Human VCAM-1 | F | CAGGCTGTGACTCCCCATTT |
R | CCCTCATTCGTCACCTTCCC |
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Lee, G.H.; Lee, S.Y.; Chae, J.Y.; Kim, J.W.; Kim, J.-H.; Jeong, H.G. Antarctic Krill Oil from Euphausia superba Ameliorates Carrageenan-Induced Thrombosis in a Mouse Model. Int. J. Mol. Sci. 2023, 24, 17440. https://doi.org/10.3390/ijms242417440
Lee GH, Lee SY, Chae JY, Kim JW, Kim J-H, Jeong HG. Antarctic Krill Oil from Euphausia superba Ameliorates Carrageenan-Induced Thrombosis in a Mouse Model. International Journal of Molecular Sciences. 2023; 24(24):17440. https://doi.org/10.3390/ijms242417440
Chicago/Turabian StyleLee, Gi Ho, Seung Yeon Lee, Ju Yeon Chae, Jae Won Kim, Jin-Hee Kim, and Hye Gwang Jeong. 2023. "Antarctic Krill Oil from Euphausia superba Ameliorates Carrageenan-Induced Thrombosis in a Mouse Model" International Journal of Molecular Sciences 24, no. 24: 17440. https://doi.org/10.3390/ijms242417440